Concentration of Bacillus spores by using silica magnetic particles

Silica particles are mainly used for the concentration of nucleic acid for diagnostic purposes. This is usually done under acidic or chaotropic conditions that will demolish most of the living organisms and prevent the application of other diagnostic tests. Here we describe the development of a method for the capturing and concentration of Bacillus spores using silica magnetic particles to enable fast and sensitive detection. We have shown that capturing various Bacilli spores via silica magnetic particles is limited, with large differences between spore batches (42 +/- 25%). The hydrophobic exosporium layer of spore limits the capture by the hydrophilic silica beads. Partial removal of Bacillus exosporium increases capture efficiency. To increase capturing efficiency without harming the spores' viability, a cationic lipid, didecyldimethylammonium bromide (DDAB), was used as a coat for the negatively charged silica particles. DDAB treatment increased capture efficiency from 42% to more than 90%. Using this method, we were able to capture as few as 100 Bacillus anthracis spores/mL with 90% efficacy. Release of captured spores was achieved by the addition of albumin. The capture and release processes were verified by plating and by flow cytometry using light scatter analysis. The method is simple, efficient, easy to operate, and fast.     

Emotional dilution of the Stroop effect: A new tool for assessing attention under emotion.

In order to gauge in a precise fashion the capture of attention by emotional stimuli, we developed a new tool that imports the classic Stroop effect into the realm of emotion. Strooping the typical emotion tasks enabled the derivation of a pure intraitem measure of attention under emotion. The results of two experiments showed that the classic Stroop effects were smaller with emotion than with neutral words, demonstrating the power of emotion to bias attention. This emotional dilution of the Stroop effect can serve as a general-purpose tool for assessing attention under emotion. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Affirmation, acknowledgment of in-group responsibility, group-based guilt, and support for reparative measures.

Three studies, 2 conducted in Israel and 1 conducted in Bosnia and Herzegovina, demonstrated that affirming a positive aspect of the self can increase one's willingness to acknowledge in-group responsibility for wrongdoing against others, express feelings of group-based guilt, and consequently provide greater support for reparation policies. By contrast, affirming one's group, although similarly boosting feelings of pride, failed to increase willingness to acknowledge and redress in-group wrongdoing. Studies 2 and 3 demonstrated the mediating role of group-based guilt. That is, increased acknowledgment of in-group responsibility for out-group victimization produced increased feelings of guilt, which in turn increased support for reparation policies to the victimized group. Theoretical and applied implications are discussed. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

The feasibility of boron removal from water by capacitive deionization

We report on the possibility of removing boron (in the form of boric acid) from water by electrochemical means. We explore capacitive de-ionization (CDI) processes in which local changes in pH near the surface of high-surface-area activated carbon fiber (ACF) electrodes during charging are utilized, in order to dissociate boric acid into borate ions which can be electro-adsorbed onto the positive electrode in the CDI cells. For this purpose, a special flow-through CDI cell was constructed in which the feed solution flows through the electrodes. Local pH changes near the carbon electrode surface were investigated using a MgCl₂ solution probe in three- (with reference) and two-electrode cells, and described qualitatively. We show that, to a certain extent, boron can indeed be removed from water by CDI.     

Selective attention improves under stress: Implications for theories of social cognition.

Three influential perspectives of social cognition entail conflicting predictions regarding the selectivity of performance under stress. According to the attention view, selectivity to the task-relevant attribute improves under stress because of reduced utilization of task-irrelevant attributes. According to the capacity-resource approach, stress depletes attentional resources wherefore selectivity fails for all but chronically accessible information. A third perspective, ironic process theory, similarly holds that selective attention fails under stress but adds that task-irrelevant information is rendered hyperaccessible. The theoretical derivations were tested in a series of experiments using 2 classes of selectivity measures, with special care taken to control for hitherto neglected factors of context. The results showed that the selectivity of attention improved under stress, consistent with the prediction of the attention view. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Counting networks with arbitrary fan-out

Summary It is shown that an acyclic smoothing network (and hence counting network) with fan-outn cannot be constructed from balancers of fan-outb ₁,...,b _k , if there exists a prime factorp ofn, such thatp does not divideb _i , for alli, 1ik. This holds regardless of the depth, fan-in or size of the network, as long as they are finite. On the positive side, a simple construction ofcyclic counting networks with fan-outn, for arbitraryn, is presented. An acyclic counting network with fan-in and fan-outp2 ^k , for any integerk0, is constructed out of 2-balancers andp-balancers. Eran Aharonson received the B.A. and M.Sc. degrees in Computer Science from the Technion, Israel Institute of Technology (Haifa, Israel) in 1989 and 1992, respectively. He is currently vice president for research and development at ART-Advanced Recognition Technolgies Ltd., a company dedicated to handwriting and voice recognition. His general research interests are distributed computation, theoretical computer science and pattern recognition. Hagit Attiya received the B.Sc. degree in Mathematics and Computer Science from the Hebrew University of Jerusalem, in 1981, the M.Sc. and Ph.D. degrees in Computer Science from the Hebrew University of Jerusalem, in 1983 and 1987, respectively. She is presently a senior lecturer at the department of Computer Science at the Technion, Israel Institute of Technology. Prior to this, she has been a post-doctoral research associate at the Laboratory for Computer Science at M.I.T. Her general research interests are distributed computation and theoretical computer science. More specific interests include fault-tolerance, timing-based and asynchronous algorithms.A preliminary version of this paper appears in proceedings of the3rd Annual ACM-SIAM Symposium on Discrete Algorithms, January 1992, pp. 104–113. This research was supported by Technion V.P.R.-B. and G. Greenberg Research Fund (Ottawa)Supported by Rashi Enterprise graduate fellowship     

Priming Effects and Strategic Influences in Social Tagging

In many social tagging systems, users can see the tags already created by others. Prior research has shown that this exposure leads users to create tags that are semantically related to the previous ones. We investigate two possible mechanisms through which this occurs, semantic priming and strategic choice. Semantic priming occurs when an existing tag subconsciously primes the user’s mind to suggest semantically related tags. In an experiment, no such effect is found, in contrast to prior research. A follow-up study shows that whether semantic priming occurs depends on whether the person uses others’ previously created tags or is just passively exposed to them. The second type of influence, strategic choice, occurs in ESP-type settings. It refers to behavior in which a user chooses words that are semantically related to an existing tag in order to increase the chances of matching one’s partner. Experimental results provide clear evidence of this strategic influence. In a follow-up study, we demonstrate that there is a meaningful difference in the tag sets that are created under the influence of strategic choice. Our work sheds light on the conditions and mechanisms through which existing tags influence subsequent tagging behavior.