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21.
H Iida Y Narita H Kado A Kashikura S Sugawara Y Shoji T Kinoshita T Ogawa S Eberl 《Canadian Metallurgical Quarterly》1998,39(1):181-189
The pyruvate dehydrogenase complex (PDC) plays a key role in the anaerobic mitochondrial metabolism of the parasitic nematode Ascaris suum. A cDNA coding for an A. suum pyruvate dehydrogenase kinase (APDK) has been cloned and sequenced from poly(A)+ RNA isolated from adult A. suum muscle.2 APDK exhibited significant sequence identity to mammalian PDKs. Nucleotide sequence analysis of the APDK cDNA revealed a 22-nucleotide spliced leader, characteristic of many nematode mRNAs, a 5'-UTR of 6 nucleotides, an open reading frame of 1197 nucleotides, and a 3'-UTR of 101 nucleotides that included a putative polyadenylation signal. The open reading frame predicted a protein of 399 amino acids with a molecular weight of 45,402 that included a putative 18-aminoacid leader peptide. Recombinant APDK (rAPDK) was functionally expressed in Escherichia coli with a his tag at its N-terminus and purified to apparent homogeneity on Ni-NTA-agarose. Recombinant APDK was a dimer and was not autophosphorylated and its activity was stimulated in the presence of APDK-deficient adult A. suum muscle PDC presumably by the binding of APDK to the dihydrolipoyl transacetylase (E2) core of the complex. After binding to the core, rAPDK activity was stimulated by elevated NADH/NAD+ and acetyl CoA/CoA ratios within the same ranges as observed for the native APDK. Immunoblotting suggested that native APDK focused as a series of 43-kDa spots (pI 6.1-6.8) on two-dimensional gels of the purified adult A. suum muscle PDC. 相似文献
22.
T Shiina G Tamiya A Oka T Yamagata N Yamagata E Kikkawa K Goto N Mizuki K Watanabe Y Fukuzumi S Taguchi C Sugawara A Ono L Chen M Yamazaki H Tashiro A Ando T Ikemura M Kimura H Inoko 《Canadian Metallurgical Quarterly》1998,47(3):372-382
The biomechanics of medialization laryngoplasty are not well understood. An excised canine larynx model was used to test the effects of various sized silicon implants. The vocal fold length, position, and tension were measured. Medialization laryngoplasty did not affect vocal fold length. At the mid-membranous vocal fold, larger shims resulted in greater medialization and tension. Medialization laryngoplasty neither medialized nor stiffened the vocal process to resist lateralizing forces. We conclude that medialization laryngoplasty provides bulk and support for defects of the membranous region of the vocal fold, but does not appear to close a posterior glottal gap. The selection of a surgical procedure to treat glottal incompetence should take into account the unique biomechanical properties of the anterior (membranous vocal folds) and posterior (cartilaginous portion) glottis. 相似文献
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24.
This paper reports the fabrication process of β-FeSi2 droplets on silicon substrates at room temperature by ArF excimer pulsed laser deposition (PLD). The chemical treatment of substrate could compensate the thermal treatment of the deposited droplets. Observations with the transmission electron microscopy revealed that the crystallization of droplet began from the surface of droplet rather than from the interface between the melt and the substrate. 相似文献
25.
Tsunehiko Sugawara Takahiro Murakami Yuichi Kuroki Mikio Ueki Takeshi Naraki Yasumasa Kato Satoshi Kanasugi 《Journal of the Society for Information Display》2007,15(1):39-47
Abstract— A new design for an FED envelope, composed of box‐shaped front glass, sheet‐like rear glass, and metal members, has been devised. This design structure is effective in reducing tensile stress induced by vacuum at the sealing points. Also, a new glass composition, a new physical tempering method, and a lead‐free hermetic sealing material have been developed. As a result, a novel lightweight spacer‐free panel structure for FEDs has been developed by integrating these new technologies. It will assist in maximizing the essential advantages of FED such as high image quality, high reliability, and low cost. 相似文献
26.
Masao Sugawara Masamitsu Kataoka Kazunori Odashima Yoshio Umezawa 《Thin solid films》1989,180(1-2):129-133
Biomimetic “ion-channel sensors” based on host-guest molecular recognition were constructed by incorporating several kinds of receptor molecules in Langmuir-Blodgett (LB) molecular assemblies deposited directly on glassy carbon electrodes. The receptors used were valinomycin, bis(crown ether)s, macrocyclic polyamine and cyclodextrin polyamine. The binding of charged stimulants (metal ions, inorganic and organic anions) to these receptors induced a marked increase (or decrease) in the ion permeability of the membranes, as detected by cyclic voltammetry using [Fe(CN)6]4- or [Ru(bpy)3]2+ as marker ion. Such a mode of response, corresponding to model “channel opening (or closing)”, can be explained on the basis of the charge-charge interaction involving the stimulant, marker ion and lipid. 相似文献
27.
This study was performed to investigate the heat extraction characteristics from shallow geothermal resources using a coaxial heat exchanger. First, a computer simulation program for a coaxial heat exchanger was checked and verified by laboratory experiments. After inspecting the effectiveness of the computer program described herein, a numerical simulation for a real scale model was conducted under the condition that the heat transfer mechanism in the stratum was heat conduction. Unsteady heat extraction characteristics are presented herein, and the effects of the tube material, inner diameter, and circular modes on the heat extraction rate are discussed. From the computer results it was found that the heat extraction performance using a coaxial heat exchanger greatly depended on the factors mentioned above in the range of the parameters covered in this study. © 2005 Wiley Periodicals, Inc. Heat Trans Asian Res, 34(7): 496–513, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/htj.20083 相似文献
28.
Takanori Sugawara Massimo Sarotto Alexey Stankovskiy Gert Van den Eynde 《Annals of Nuclear Energy》2011
The XT-ADS, an accelerator-driven system for an experimental demonstration, has been investigated in the framework of IP EUROTRANS FP6 project. In this study, the sensitivity and uncertainty analyses were performed to comprehend the reliability of the XT-ADS neutronic design. 相似文献
29.
30.
We perform a theoretical analysis of frictional-force microscopy(FFM) images of a cleaved graphite surface. The Tomlinson
model with a single-atom tip is powerful for reproducing experimental FFM images. A mapping relation between the tip atom
position and the cantilever basal position obtained by a stable equilibrium condition, gives a clear explanation of the physical
meaning of FFM image patterns.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献