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RY Lin DC Saha CJ Bernstein ME Astiz EC Rackow 《Canadian Metallurgical Quarterly》1996,27(5-6):303-317
Sepsis and trauma have similarities in their immunopathologic profiles. Both conditions can result in multi-system organ failure which is sometimes associated with cytokine generation and inflammatory cell activation. Furthermore, decreases in peripheral blood monocyte expression of HLA-DR have been noted in both human sepsis and trauma. However, the magnitude, onset, and time course of such stimuli are often difficult to ascertain in human studies. Thus, to study a more detailed in vivo immunologic profile in these conditions, rat models were employed. Our aim was to describe and analyze cytokine and peripheral blood immunophenotype patterns in bacterially induced rat sepsis and to compare this to rat ischemia-reperfusion injury. Sprague-Dawley rats underwent either bacterial injection with enterotoxin producing Staphylococcus aureus or hind limb ischemia/ reperfusion. Two bacterial doses which were either lethal or sublethal at 24-48 hours were utilized. Peripheral blood neutrophils and B-lymphocytes were studied for expression of beta-integrins (CD11b and CD11b/c) and I-A, respectively, using flow cytometry. Corresponding plasma levels of TNF alpha and interferon gamma were measured by ELISA. At 24 hr, a lethal bacterial lethal bacterial dose injection resulted in significantly higher levels of neutrophil CD11b/c expression (p < 0.005) compared with ischemia-reperfusion treatment. B-cell I-A expression was also higher in lethal sepsis. Gamma interferon levels were significantly higher in lethal sepsis compared with ischemia-reperfusion (p = 0.005). Studies over time showed that CD11b expression and interferon gamma were both more marked at 6 hr than at 24 hr in lethal sepsis. This pattern was not observed in sublethal sepsis or in ischemia-reperfusion. CD11b/c expression on the other hand remained elevated at comparable levels at 6 and 24 hr in lethal sepsis. B-cell I-A expression in ischemia-reperfusion and sublethal sepsis decreased at 24 hr compared with baseline. Lethal sepsis in rats injected with enterotoxin producing staphylococcus results in phasic alterations in neutrophil CD11b and plasma interferon levels prior to death. In analogy to the findings of monocyte decreases in DR expression observed in human trauma and sepsis, rat B-cell I-A expression showed decreases in sublethal sepsis as well as in ischemia-reperfusion injury. However, this was not observed in lethal sepsis. These findings have implications in understanding the immunologic/inflammatory changes observed in human sepsis and trauma. 相似文献
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本文报道采用流延法制备的热释电厚膜陶瓷材料直接装配的单元红外探测器,该方法大大简化了目前探测器的制备工艺,极大地提高了材料的利用率,器件的性能也达到了目前探测器的水平。 相似文献
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激光扫描三角法是近十年发展起来的三维传感新技术,其实现扫描测量的方法可分为两类:一是采用阵列器件,用相应的同步扫描机构实现;另一则采用单通道器件,由扫描装置进行步进扫描实现。虽然这种技术在陆地及空气系统中已得到广泛应用,但在水下却存在着许多问题。本文提出了一种新型空间差频扫描测量方式,并对扫描测量原理、测量周期、分辨率及速率进行了深入讨论,最后的应用结果显示了这种新型方式用于水下三维传感的特殊优越性。 相似文献
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Four ferritic spheroidal graphite (SG) cast irons of different graphite nodule interparticle spacings were prepared for a
tensile experiment. The results indicate a decreasing ductility with increasing nodule spacing. Transition from pure dimple
to a mixed mode of dimple and cleavage occurs when the nodule spacing reaches a critical value. The data of the S.G. cast
iron possessing the largest nodule spacing reveal that the ductility is increased by raising the superposed hydrostatic pressure.
The mixed mode of dimple and cleavage fractures of this SG cast iron, which occurs under normal pressure, is replaced by pure
dimple fracture under all the hydrostatic pressure conditions. These results are analyzed in this article in terms of the
triaxiality ratio at the ferrite center region. The higher the local triaxiality ratio, the lower the ductility and the larger
the chance for cleavage fracture to occur. 相似文献