Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.     

An adaptive learning algorithm for a wavelet neural network

Abstract: An optimal online learning algorithm of a wavelet neural network is proposed. The algorithm provides not only the tuning of synaptic weights in real time, but also the tuning of dilation and translation factors of daughter wavelets. The algorithm has both tracking and smoothing properties, so the wavelet networks trained with this algorithm can be efficiently used for prediction, filtering, compression and classification of various non-stationary noisy signals.     

Comparison of some characteristics of thin‐film‐electroluminescent ZnS: Er,F planar and edge emitters

Abstract— The following main differences have been revealed in the characteristics of an edge thin‐film‐electroluminescent ZnS: Er,F emitter compared to those of a similar planar emitter: (1) the 1.535‐μm band more highly dominates over other bands in the EL spectrum; (2) the voltage (V) dependence of the intensity of this band is the strongest; (3) the 1.535‐μm band narrows with increasing voltage and its frequency. The above differences are explained, firstly, by smaller optical losses in the ZnS: Er,F film for the near‐infrared emission than for the visible one and, secondly, by an optical amplification over the 1.535‐μm band in the edge emitter.     

Flow through regular polygonal channels

A general method for analyzing viscous flow through regular polygonal channels is presented, based upon the assumption that the flow rate through regular polygonal sections equals that through circular sections of the same area. In addition we offer alternatives which apply specifically to equilaterial triangles and to squares.     

Ambiguity Resolution in Systems Using Omega for Position Location

The lane ambiguity problem prevents the utilization of the Omega system for many applications such as locating buoys and balloons. The method of multiple lines of position introduced herein uses signals from four or more Omega stations for ambiguity resolution. The coordinates of the candidate points are determined first through the use of the Newton iterative procedure. Subsequently, a likelihood function is generated for each point, and the ambiguity is resolved by selecting the most likely point. The method was tested through simulation.