Performance model of interactive video-on-demand systems

An interactive video-on-demand (VoD) system allows users to access video services, such as movies, electronic encyclopedia, interactive games, and educational videos from video servers on a broadband network. This paper develops a performance evaluation tool for the system design. In particular, a user activity model is developed to describe the usage of system resources, i.e., network bandwidth and video server usage, by a user as it interacts with the service. In addition, we allow batching of user requests, and the effect of such batching is captured in a batching model. Our proposed queueing model integrates both the user activity and the batching model. This model can be used to determine the requirements of network bandwidth and video server and, hence, the trade-off in communication and storage costs for different system resource configurations     

A genetic algorithm for multiple molecular sequence alignment

MOTIVATION: Multiple molecular sequence alignment is among the most important and most challenging tasks in computational biology. The currently used alignment techniques are characterized by great computational complexity, which prevents their wider use. This research is aimed at developing a new technique for efficient multiple sequence alignment. APPROACH: The new method is based on genetic algorithms. Genetic algorithms are stochastic approaches for efficient and robust searching. By converting biomolecular sequence alignment into a problem of searching for optimal or near-optimal points in an 'alignment space', a genetic algorithm can be used to find good alignments very efficiently. RESULTS: Experiments on real data sets have shown that the average computing time of this technique may be two or three orders lower than that of a technique based on pairwise dynamic programming, while the alignment qualities are very similar. AVAILABILITY: A C program on UNIX has been written to implement the technique. It is available on request from the authors.     

Development of a monoclonal antibody to 6 beta-hydroxycortisol and its application in an enzyme-linked immunosorbent assay (ELISA) for 6 beta-hydroxycortisol in urine

A novel monoclonal antibody to 6 beta-hydroxycortisol (6 beta-OHC) was generated and incorporated into an antigen-coated indirect enzyme-linked immunosorbent assay (ELISA) using 6 beta-OHC-protein conjugate as the steroid-coating antigen. The monoclonal antibody is specific to 6 beta-OHC and 6 beta-OHC-3-carboxymethyloxime. Cross-reactivity with other structurally related steroids such as cortisol, cortisone, and 6 beta-hydroxycortisone was less than 10%. Two different clones (clone 5C1 and 19F) of the monoclonal anti-6 beta-OHC antibody have been developed, each with slightly different sensitivity and specificity. The sensitivity of the MAb clones was not significantly improved when compared to the rabbit polyclonal antibodies in this study, but still within the accepted detection limit for 6 beta-OHC in both human and laboratory animals. The assay had a detection limit of 200 ng/ml, an intraassay variation of 6.4% and an interassay variation of 7.3%. The application of the anti-6 beta-OHC-MAb-based-ELISA was tested by measuring the urinary output of 6 beta-OHC in human before and after enzyme induction by rifampicin treatment. The mean 24-h urine output of 6 beta-OHC in human subjects was 485 +/- 100 micrograms and 1478 +/- 281 micrograms before and after rifampicin administration, respectively. In conclusion, the monoclonal anti-6 beta-OHC antibody developed in this study has the required specificity and sensitivity as an alternative method for measuring urinary 6 beta-OHC in the detection of enzyme induction or enzyme inhibition of CYP3A in humans and laboratory animals.     

The MiniWiD-Coater. III. Effect of Application Temperature on the Dissolution Profile of Sustained-Release Theophylline Pellets Coated with Eudragit Rs 30 D

Theophylline pellets were coated with Eudragit RS 30 D in a miniature fluid-bed pan coater called MiniWiD developed recently. The dispersions were plasticized with varying amounts of triethyl citrate (TEC), dibutyl phthalate (DBP), and polyethylene glycol 6000 (PEG) and applied at different temperatures ranging from 25 to 45 °C. Theophylline release was tested by dissolution using the USP Apparatus 2 (paddle) in 0.1 N hydrochloric acid under sink conditions over 6 hours.

At a coating level of 4 % (0.7 mg/cm²) sustained-release profiles were obtained from dispersions plasticized with TEC or DBP. By reducing the amount of plasticizer from 20 to 10%, films with higher permeabilities were obtained. This effect was compensated by tempering the pellets at 50 deg;C for 24 hours. The coating temperature had little effect on the dissolution profiles of TEC-plasticized films and no effect on films with DBP.

Coatings plasticized with 20% PEG were applied at temperatures ranging from 25 to 45 °C. These films required a coating level of about 18 % (3.3 mg/cm²) to provide comparable sustained-release properties. In contrast to DBP and TEC, a strong influence of the coating temperature on the release rates was observed in which higher temperatures led to slower release rates. This behavior can be explained by the minimum film-forming temperature (MFT). Since PEG does not lower the MFT of Eudragit RS 30 D, the application of these films below the MFT of 45 °C is associated with a lower degree of film formation.     

On the computation of conjugacy classes of Chevalley groups

In this paper an algorithm is presented to determine the number of semisimple conjugacy classes for a given centralizertype for the Chevalley groupsSL _n(q) andSU _n(q). Moreover we give the exact number of regular semisimple classes of this groups and a summary of results on the generic classnumber of exceptional Chevalley groups of adjoint type.The first author acknowledges financial support by a grant of the Deutsche Forschungsgemeinschaft     

Compile-time copy elimination

Single-assignment and functional languages have value semantics that do not permit side-effects. This lack of side-effects makes automatic detection of parallelism and optimization for data locality in programs much easier. However, the same property poses a challenge in implementing these languages efficiently. This paper describes an optimizing compiler system that solves the key problem of aggregate copy elimination. The methods developed rely exclusively on compile-time algorithms, including interprocedural analysis, that are applied to an intermediate data flow representation. By dividing the problem into update-in-place and build-in-place analysis, a small set of relatively simple techniques—edge substitution, graph pattern matching, substructure sharing and substructure targeting—was found to be very powerful. If combined properly and implemented carefully, the algorithms eliminate unnecessary copy operations to a very high degree. No run-time overhead is imposed on the compiled programs.     

Irrigation Modelling in the Context of Basin Water Resources

Management practices for irrigation schemes can often be improved by modelling the behaviour of a scheme and by evaluating its efficiency statistics. However, looking at an irrigation scheme without regard for other water uses within the river basin may not be effective. Efficiencies computed in this way are local and, since water may be used many times within the same basin, may not be realistic. Changes in water uses (e.g. irrigation, industrial, power production, urban water supply, navigation, environmental, recreational) will often have effects on other uses; and changes in irrigation schemes (management,structural, crop pattern) may affect other users within the basin. In addition, all water users will be affected by external changes such as changes in land cover or in climate. As demand for water increases, these links become more important until, at some stage, there is insufficient water for all users and hard choices must be made. Hydrological modelling is a tool that can be used to relate irrigation schemes to the other water uses within a river basin and can help in assessing real productivities and in evaluating alternative patterns of water usage. This paper discusses the techniques available to simulate irrigation schemes within overall basin water resources using, as an example, an intensively utilized basin in western Turkey.