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51.
Manitha B. Nair H. K. Varma Annie John 《Journal of materials science. Materials in medicine》2009,20(1):251
Current treatment strategies for the repair or replacement of bone use synthetic implants with stem cells and their progeny––a new approach to address unmet medical needs. This study has evaluated the effect of a silica-coated bioactive ceramic, namely HASi in comparison to hydroxyapatite (HA) on the adhesion, proliferation and osteogenic differentiation of goat bone marrow-derived mesenchymal stem cells in vitro in a prolonged culture of 28 days. The cellular activities were significantly enhanced on HASi signifying the role of silica to stimulate osteoblast cells. The fabrication of such a ‘cell-ceramic construct using autologous MSCs’ is aimed for the transplantation to a large bone defect site in the goat femur model which still remains a formidable challenge in Orthopedic surgery. 相似文献
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Batch sorbitol to sorbose bioconversion by Acetobacter suboxydans using initial sorbitol concentration (S0 = 100 g/L) yielded a productivity of 10.11 g/L‐h and 98.6% conversion in 10 h time. The batch kinetics was then used to develop an unstructured mathematical model. Model parameters were identified using a nonlinear regression technique assisted by a computer program which minimized the deviation between the model predictions and actual batch experimental data. F test indicated 99% confidence on the prediction of model using optimized parameters. The batch model was eventually extrapolated to identify nutrient feeding strategies to maintain constant noninhibitory sorbitol supply and eliminate substrate limitation for fed‐batch fermentation in order to improve the sorbose productivity. The adequacy of the fed‐batch model was established by excellent agreement between experimental data and model simulation (except towards the end of fermentation). 相似文献
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We have investigated laser action in a binary mixture of dyes, Rh-6G and DCM, resulting in tunable laser emission over an extended frequency region. The two dyes absorb the same pump radiation but fluoresce over frequency ranges that are shifted with respect to each other, thereby resulting in extended tunability. Following a time-dependent analysis of a rate-equation model that describe the operation of such a laser, theoretical estimates for optimum dye concentrations and the corresponding extension of the laser tuning range have been obtained. 相似文献
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Balaji T Acharya RN Nair AG Reddy AV Rao KS Naidu GR Manohar SB 《The Science of the total environment》2000,253(1-3):75-79
The concentrations of some elements in a few varieties of cereals and pulses are determined by Instrumental Neutron Activation Analysis using a single comparator method (k0-standardised NAA method). A total of 15 elements are measured. The method was validated by analysing the Standard Reference Material (SRM-1571) of NIST; the results are within +/-10% of the reported values for the majority of the elements. The measured concentrations of major and minor elements are analysed in terms of the average intake of mineral content and the role of these elements in terms of the nutritional value. 相似文献
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BACKGROUND: Synthetic homopyrimidine peptide nucleic acids (PNAs) can bind complementary targets in double-stranded DNA, generating strand-displacement complexes, and so offering an opportunity to modulate specific gene expression. Several issues remain to be addressed before these attributes can be exploited in vivo, however. RESULTS: The kinetics of the interaction between a homopyrimidine PNA and a complementary homopurine target on double-stranded DNA were analyzed in the presence or absence of a preformed strand-displacement complex proximal to the target. The complex was established under low salt conditions by the binding of a different homopyrimidine PNA to a target situated adjacent to the first PNA target. These two targets were placed next to each other on opposite strands at distances of 0, 2, 4 and 8 base pairs apart. The presence of a preformed strand-displacement complex near the target accelerates the binding of PNA to double-stranded DNA in a salt-dependent manner. The influence of salt on the binding rates was also examined. The binding rate is increased by a factor of 1 x exp(70[NaCl]), that is, 16-fold at 40 mM NaCl and more than 10(4)-fold if extrapolated to 140 mM NaCl. This effect is significantly reduced if the two targets are 2 base pairs apart and completely absent if the distance is 4 base pairs or more. CONCLUSIONS: The perturbation of the DNA helix imposed by a PNA strand-displacement complex only propagates a few base pairs. It is therefore possible to target sites in the immediate vicinity of strand invasion complexes specifically. The results presented have implications for the mechanism of strand displacement and for the application of PNA in a genomic context. 相似文献
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N. Radhakrishnan Nair N. M. Claramma N. M. Mathew Sabu Thomas S. Someswara Rao 《应用聚合物科学杂志》1995,55(5):723-731
The flow behavior of liquid natural rubber (LNR) with reference to the effect of molecular weight, shear rate, and temperature has been studied. LNR samples were prepared by thermal depolymerization of rubber (NR) through the combined effect of mechanical, chemical, and thermal energies. High molecular weight samples, especially at lower temperatures, showed pseudoplastic behavior, whereas the low molecular weight samples were Newtonian at all temperatures. The viscosity of LNR considerably decreases with increase in temperature. The activation energy of flow was also calculated. © 1995 John Wiley & Sons, Inc. 相似文献
60.
A complex perovskite oxide Ba2HoNbO6 has been developed as a non-reacting substrate for YBa2Cu3O7– superconducting film with lattice constant a = 8.3905 Å. The dielectric constant (30) and loss factor value (5 × 10–3) of the material are in the range suitable for its use as substrate for microwave applications. A YBa2Cu3O7–delta; superconducting thick film dip coated on Ba2HoNbO6 substrate gave a T
c(0) of 92 K and current density of 1.2 × 104 A cm–2. 相似文献