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U Russ C Balser W Scholz U Albus HJ Lang A Weichert BA Sch?lkens H G?gelein 《Canadian Metallurgical Quarterly》1996,433(1-2):26-34
The inhibitors of the Na+/H+-exchange (NHE1) system Hoe 694 and Hoe 642 possess cardioprotective effects in ischaemia/reperfusion. It is assumed that these effects are due to the prevention of intracellular sodium (Nai) and calcium (Cai) overload. The purpose of the present study was to investigate the effects of Hoe 642 on intracellular pH, Na+ and Ca2+ (pHi, Nai and Cai) in isolated rat ventricular myocytes under anoxic conditions or in cells in which oxidative phosphorylation had been inhibited by 1.5 mmol/l cyanide. In cells which were dually loaded with the fluorescent dyes 2, 7-biscarboxyethyl-5,6-carboxyfluorescein (BCECF) and Fura-2, anoxia caused acidification of the cells (from pHi 7.2 to pHi 6.8) and an increase in Cai from about 50 nmol/l to about 1 micromol/l. The decrease in pHi began before the cells underwent hypoxic (rigor) contracture, whereas Cai only began to rise after rigor shortening had taken place. After reoxygenation, pHi returned to its control value and Cai oscillated and then declined to resting levels. It was during this phase that the cells rounded up (hypercontracture). When 10 micromol/l Hoe 642 was present from the beginning of the experiment, pHi and Cai were not significantly different from control experiments. At reoxygenation, pHi did not recover, but Cai oscillated and returned to its resting level. To monitor Nai, the cells were loaded with the dye SBFI. After adding 1.5 mmol/l cyanide or 100 micromol/l ouabain, Nai increased from the initial 8 mmol/l to approximately 16 mmol/l. Hoe 642 or Hoe 694 (10 micromol/l) did not prevent the increase in Nai. In contrast, the blocker of the persistent Na+ current R56865 (10 micromol/l) attenuated the CN--induced rise in Nai. The substance ethylisopropylamiloride was not used because it augmented considerably the intensity of the 380 nm wavelength of the cell's autofluorescence. In conclusion, the specific NHE1 inhibitor Hoe 642 did not attenuate anoxia-induced Cai overload, nor CN--induced Nai and Cai overload. Hoe 642 prevented the recovery of pHi from anoxic acidification. This low pHi maintained after reoxygenation may be cardioprotective. Other possible mechanisms of NHE1 inhibitors, such as prevention of Ca2+ overload in mitochondria, cannot be ruled out. The increase in Nai during anoxia is possibly due to an influx of Na+ via persistent Na+ channels. 相似文献
45.
Poly(diaryl diazosulfide)s and model diaryl diazosulfides of the general structure aryl-N?N? S-aryl' are tested for their thermostability. Thermolysis in solution is followed by means of UV spectroscopy. At the beginning of the decay most model diazosulfides follow first-order kinetics, but show an acceleration of the decay rate in the further course of the thermolysis. In contrast to the models the decomposition of the corresponding polymers cannot be described by first-order kinetics, except one example ( P 1 ). Thermogravimetry studies show weight losses of 8–12% in the range between 80 and 150°C depending on the structure of the models and polymeric diazosulfides. These values correspond with the content of azo function in these compounds, therefore it is assumed that nitrogen is split off under these reaction conditions. 相似文献
46.
本文用ASAP2000吸附仪测定了吸附性能优异的沥青基高比表面积新型活性碳(PHAC)的BET比表面积、用BJH和DRS法分析了其孔隙结构参数,用XPS测定了PHAC的表面元素组成及含氧官能团,对PHAC的表面形态结构进行了SEM和TEM观察。研究表明:PHAC具有高度发达且均匀的微孔结构,其表面含有一定量的C-O-C、C-OH、C=O、O=C-O等多种类型的含氧官能团。 相似文献
47.
Mandrell D Truong L Jephson C Sarker MR Moore A Lang C Simonich MT Tanguay RL 《Journal of laboratory automation》2012,17(1):66-74
The potential of the developing zebrafish model for toxicology and drug discovery is limited by inefficient approaches to manipulating and chemically exposing zebrafish embryos-namely, manual placement of embryos into 96- or 384-well plates and exposure of embryos while still in the chorion, a barrier of poorly characterized permeability enclosing the developing embryo. We report the automated dechorionation of 1600 embryos at once at 4 h postfertilization (hpf) and placement of the dechorionated embryos into 96-well plates for exposure by 6 hpf. The process removed ≥95% of the embryos from their chorions with 2% embryo mortality by 24 hpf, and 2% of the embryos malformed at 120 hpf. The robotic embryo placement allocated 6-hpf embryos to 94.7% ± 4.2% of the wells in multiple 96-well trials. The rate of embryo mortality was 2.8% (43 of 1536) from robotic handling, the rate of missed wells was 1.2% (18 of 1536), and the frequency of multipicks was <0.1%. Embryo malformations observed at 24 hpf occurred nearly twice as frequently from robotic handling (16 of 864; 1.9%) as from manual pipetting (9 of 864; 1%). There was no statistical difference between the success of performing the embryo placement robotically or manually. 相似文献
48.
阐述了基于B/S模式开发湖南高速铁路职业技术学院财务管理信息系统,并详细介绍了系统的功能需求和数据库设计,以及主要功能模块的设计与实现代码。该系统将Web技术和数据库技术很好的融合在一起,使数据库成为Web的重要组成部分,实现了数据库技术和网络技术的有机结合。 相似文献
49.
任意波形发生器是现代电子测量领域应用最为广泛的一种通用信号源;为了更好地满足人机交互,介绍了一套基于μC/GUI和SOPC技术的任意波形发生器人机交互系统设计方案;系统采用Avalon突发模式设计一个通用的LCD控制器,实现触摸屏为人机交互输入接口,分析并完成μC/GUI的移植,最后完成仪器的图形界面设计;整个系统采用SOPC技术集成到单片FPGA芯片内部,既降低开发成本,又提高系统的稳定性;实验结果表明基于μC/GUI设计的界面简洁友好,触摸屏操作方便直观,整个系统运行稳定,任务能够实时处理;该系统占用资源少,成本低,集成度高,稳定性强,具有一定的实用价值。 相似文献
50.
This paper presents an indirect adaptive controller combined with hysteresis compensation to achieve high accuracy positioning control of piezoceramic actuators and illustrates the results with an atomic force microscope (AFM) application. A dynamic model of a piezoceramic actuator system in AFM is derived and analyzed. A feedforward controller based on the Preisach model is proposed to compensate for the nonlinear hysteresis effects. Then an indirect adaptive controller is designed to achieve desired tracking performance as well as deal with the uncompensated nonlinearity from hysteresis and the system parameter variation due to creep. Experimental results indicate that the proposed controller can significantly improve the positioning control accuracy of the piezoceramic actuator as well as achieve high image quality of the AFM system. The maximum scanning error was reduced from 2µm to 0.3µm in comparison with a proportional‐integral‐derivative (PID) controller. Copyright © 2011 John Wiley and Sons Asia Pte Ltd and Chinese Automatic Control Society 相似文献