Deterioration of protein fraction by Maillard reaction in dietetic milks.

The development of the Maillard reaction in pasteurized, UHT and in-bottle sterilized dietetic milks was studied. In these products damage caused by heat treatments could increase as a result either of the addition of various ingredients or of manufacturing processes that alter their content of reducing carbohydrates. Protein damage was evaluated by measuring furosine by reversed-phase ion-paired HPLC. The levels of furosine detected made it possible to assess the amounts of biologically unavailable lysine. In all milks analysed blocked lysine values were < 340-350 mg/g total lysine, the level at which lysine becomes the limiting amino acid in milk. Pasteurized dietetic milks had levels of blocked lysine similar to that in ordinary pasteurized cows' milk. In some UHT and in-bottle sterilized dietetic milks their different composition resulted in an increase in the blocked lysine content. In some in-bottle sterilized milks, protein damage greatly reduces the beneficial effects of milk as a dietary supplement. Lactose-free milks, which are more susceptible to protein deterioration because of their higher content of reducing carbohydrates, were also analysed after storage at 20 degrees C and at < or = 4 degrees C. At the end of their recommended storage times, they contained limited amounts of blocked lysine only if they had been stored at < or = 4 degrees C.     

Measurement of mechanical loads in large wind turbines: Problems on calibration of strain gage bridges and analysis of uncertainty

The paper discusses the complexity of calibration of strain gage full bridges applied to measure mechanical loads in large wind turbines, when direct application of calibration loads is not feasible. In particular, at first, it presents a generalized static‐dynamic mechanical model which allows to calibrate the strain gage full bridges using its own unbalanced masses to generate known reference inputs. Then, the paper discusses the uncertainty associated to such a calibration, according to the ISO/IEC Guide 98‐3:2008 “Guide to the Expression of Uncertainty in Measurement”. The uncertainty of the reference input and the following calibration is discussed, which is often larger than the target set by the standard IEC‐61400‐13 used for wind turbine type certification. The paper comments on the attainable range of calibration which is rather limited with respect to expected load range in operation. Even if calibration should take place in isothermal effects, this is not always the case in real world practice. Therefore, the thermal effects on strain gage bridges are also discussed, putting into evidence its influence on calibration uncertainty both for full bridges in T configuration and in parallel configuration.