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41.
A novel method for three-dimensional observation of the vascular networks in the whole mouse brain 总被引:1,自引:0,他引:1
A novel method for acquiring serial images suitable for three-dimensional reconstruction of vascular networks in the whole brain of mouse was developed. The brain infused with a White India ink-gelatin solution was fixed and embedded in paraffin containing Sudan Black B through xylene also containing Sudan Black B. Each sliced surface of the paraffin block was coated with liquid paraffin and its image was serially acquired. Coating with liquid paraffin extremely improved the quality of the image. The series of serial images was free of distortion and a three-dimensional image was reconstructed without the problem of the alignment and registration of adjacent images. The volume-rendered image indicated three-dimensional distribution of blood vessels in a whole brain. No ghost or shadow was observed on a volume-rendered image of the White India ink-gelatin infused brain. The z-axial resolution examined on the orthogonal sections reconstituted from serial images obtained at an interval of 5 mum showed no cross talk, indicating that the z-axial resolution was no larger than 5 mum. A proper understanding of the vascular system in a whole brain is indispensable to reveal the development of the vascular system in the brain of normal and genetically manipulated mouse and vascular alterations in pathological situation, such as stroke and neurodegenerative disease. Although simple and inexpensive, this method will provide fundamental information on the vascular system in a whole brain. 相似文献
42.
Composite bodies in the system Al–Zr–C, with about 95% relative density, were obtained by heating the compact body of powder mixture consisting of Al and ZrC (5 : 1 mol %) in Ar at 1100–1500°C for various lengths of time. Components of the material heated at more than 1200°C were Al, Al3Zr, ZrC and AlZrC2. The Al3Zr exhibited plate-like aggregation, and its size increased with increasing temperature. In the material heated at 1500°C for 1 h, the largest plate-like Al3Zr aggregation was 2000 m long and 133 m thick. Then the AlZrC2 was present as well-proportioned hexagonal platelet particles with a 8–9 m diameter and a 1–2 m thickness in the interior of the plate-like Al3Zr aggregation and Al matrix phase. The average three-point bending strength of the bodies was 140–190 MPa, and the maximum strength was 203 MPa in the body heated at 1300°C for 1 h. The body heated at 1500°C for 1 h showed high oxidation resistivity to air up to 1000°C. 相似文献
43.
Conceptually, there should be a brief interval in time when newly secreted proteins "pile up" at secretory sites just outside the membrane of ameloblasts. Indeed, previous cytochemical studies have suggested that glycosylated and/or sulfated glycoproteins accumulate at enamel growth sites. Colloidal gold lectin cytochemistry and immunocytochemistry with antibodies to enamel proteins and phosphoserine, combined with cycloheximide and brefeldin A to inhibit protein synthesis and secretion, were applied to characterize the distribution of newly formed proteins at enamel interrod and rod growth sites. Although enamel growth sites show a "rarefied" appearance, the results indicate that one or more subclasses of enamel proteins accumulate near the cell surface at sites where elongation of enamel crystallites contributes to thickening of the enamel layer. These proteins are glycosylated and/or phosphorylated and, at least in the case of the glycosylated ones, are rapidly processed after they are released extracellularly. In contrast, immunolabeling for amelogenins is generally weaker near the cell surface and more intense at a short distance away from the site where crystallites elongate. The data suggest that the enamel proteins accumulating at growth sites likely belong to the non-amelogenin category and play a transient role in promoting the lengthening of crystallites. It is concluded that areas near the ameloblast membrane where certain enamel proteins accumulate in fact constitute the equivalent of a mineralization front. 相似文献
44.
Vasileios Belagiannis Xinchao Wang Horesh Beny Ben Shitrit Kiyoshi Hashimoto Ralf Stauder Yoshimitsu Aoki Michael Kranzfelder Armin Schneider Pascal Fua Slobodan Ilic Hubertus Feussner Nassir Navab 《Machine Vision and Applications》2016,27(7):1035-1046
Multiple human pose estimation is an important yet challenging problem. In an operating room (OR) environment, the 3D body poses of surgeons and medical staff can provide important clues for surgical workflow analysis. For that purpose, we propose an algorithm for localizing and recovering body poses of multiple human in an OR environment under a multi-camera setup. Our model builds on 3D Pictorial Structures and 2D body part localization across all camera views, using convolutional neural networks (ConvNets). To evaluate our algorithm, we introduce a dataset captured in a real OR environment. Our dataset is unique, challenging and publicly available with annotated ground truths. Our proposed algorithm yields to promising pose estimation results on this dataset. 相似文献
45.
Yoshimitsu Aoki Shuji Hashimoto Masahiko Terajima Akihiko Nakasima 《The Visual computer》2001,17(2):121-131
We propose a prototype of a facial surgery simulation system for surgical planning and the prediction of facial deformation.
We use a physics-based human head model. Our head model has a 3D hierarchical structure that consists of soft tissue and the
skull, constructed from the exact 3D CT patient data. Anatomic points measured on X-ray images from both frontal and side
views are used to fire the model to the patient's head.
The purposes of this research is to analyze the relationship between changes of mandibular position and facial morphology
after orthognathic surgery, and to simulate the exact postoperative 3D facial shape. In the experiment, we used our model
to predict the facial shape after surgery for patients with mandibular prognathism. Comparing the simulation results and the
actual facial images after the surgery shows that the proposed method is practical. 相似文献
46.
N. Ishikawa Y. Chimi O. Michikami T. Hashimoto T. Kambara R. Neumann A. Iwase 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》2005,230(1-4):136-141
Thin films of EuBa2Cu3Oy oxide superconductor have been irradiated with high energy heavy ions (80 MeV I, 125 MeV Br, 1.1 GeV Mo and 3.5 GeV Xe) having same electronic stopping power, Se, in order to investigate the ion-velocity dependence of the electronic excitation effects under the constant electronic energy deposition. Although Se is constant, a strong reduction in the irradiation effect on lattice parameter with increasing ion-velocity is observed in the low ion-velocity region around E 1 MeV/nucleon, while the ion-velocity dependence is hardly observed in the high ion-velocity region of E > 10 MeV/nucleon. If the observed velocity-dependence is assumed to be due to the change in the fraction of Se contributing to defect creation, the fraction in the low velocity region (E 0.6 MeV/nucleon) is estimated to be about two times larger than that in the high velocity region (E > 10 MeV/nucleon). 相似文献
47.
This paper proposes a transformation method that serves the trade‐off between the modelling complexity and accuracy of multi‐variable Takagi‐Sugeno fuzzy inference operator‐based modelling (TS fuzzy modelling). The relation between the number of fuzzy rules and the modelling accuracy is defined in the paper. The proposed transformation method is capable of finding the minimal number of fuzzy rules for a given accuracy of a given TS fuzzy model. A case study, focusing on a benchmark problem of fault diagnosis, developed in the framework of EC‐founded Research Training Network DAMADICS, of an actuator in a sugar factory, is presented to provide feasibility of the proposed method. 相似文献
48.
Tomohiro Fujii Jun Nishikawa Soichiro Fukuda Naoto Kubota Junzo Nojima Koichi Fujisawa Ryo Ogawa Atsushi Goto Koichi Hamabe Shinichi Hashimoto Aung Phyo Wai Hisashi Iizasa Hironori Yoshiyama Kohei Sakai Yutaka Suehiro Takahiro Yamasaki Taro Takami 《International journal of molecular sciences》2022,23(18)
DNA methylation of both viral and host DNA is one of the major mechanisms involved in the development of Epstein–Barr virus-associated gastric carcinoma (EBVaGC); thus, epigenetic treatment using demethylating agents would seem to be promising. We have verified the effect of , which was discovered by screening for demethylating agents. MC180295 inhibited cell growth of the EBVaGC cell lines YCCEL1 and SNU719 in a dose-dependent manner. In a cell cycle analysis, growth arrest and apoptosis were observed in both YCCEL1 and SNU719 cells treated with MC180295. MKN28 cells infected with EBV were sensitive to MC180295 and showed more significant inhibition of cell growth compared to controls without EBV infection. Serial analysis of gene expression analysis showed the expression of genes belonging to the role of BRCA1 in DNA damage response and cell cycle control chromosomal replication to be significantly reduced after MC180295 treatment. We confirmed with quantitative PCR that the expression levels of BRCA2, FANCM, RAD51, TOP2A, and CDC45 were significantly decreased by MC180295. LMP1 and BZLF1 are EBV genes with expression that is epigenetically regulated, and MC180295 could up-regulate their expression. In conclusion, MC180295 inhibited the growth of EBVaGC cells by suppressing DNA repair and the cell cycle. MC180295相似文献
49.
Elisabete Yurie Sataque Ono Marcelo da Silva Elisabete Hiromi Hashimoto Édio Vizoni Osamu Kawamura Yoshitsugu Sugiura 《Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment》2013,30(11):1392-1399
Based on fungal and fumonisin contamination of 870 freshly harvested samples, the quality of corn used by processing industries in the Northern region of Paraná State, Brazil (2003 and 2004 crop-year) was evaluated. Sampling was carried out for each crop at two points in the production chain, i.e. at reception by the processors and at the pre-drying step. Corn samples were more frequently contaminated with Fusarium sp. (100%) and Penicillium sp. (84.1–95.3%) than Aspergillus sp. (5.6–19.8%). Fumonisin B1 (FB1) was detected in all samples from the two points in both crop-years. FB1 levels ranged 0.02–11.83 µg g?1 in the reception and 0.02–10.98 µg g?1 in the pre-drying samples of the 2003 crop. Samples from the 2004 crop showed FB1 levels ranging 0.03–12.04 µg g?1 in the reception and 0.06–7.74 µg g?1 in the pre-drying samples. FB2 levels ranged 0.02–5.25 µg g?1 in the reception and 0.01–7.89 µg g?1 in the pre-drying samples (2003 crop-year). In samples from the 2004 crop, FB2 levels ranged 0.02–6.12 µg g?1 in the reception and 0.05–3.47 µg g?1 in the pre-drying samples. Low fumonisin levels were detected in most corn samples used by processors in the Northern region of Paraná State, showing a decreasing trend in fumonisin contamination over the years. 相似文献
50.