全文获取类型
| 收费全文 | 131篇 |
| 免费 | 2篇 |
专业分类
| 化学工业 | 49篇 |
| 机械仪表 | 4篇 |
| 矿业工程 | 1篇 |
| 能源动力 | 2篇 |
| 轻工业 | 24篇 |
| 无线电 | 3篇 |
| 一般工业技术 | 30篇 |
| 冶金工业 | 6篇 |
| 自动化技术 | 14篇 |
出版年
| 2023年 | 7篇 |
| 2022年 | 17篇 |
| 2021年 | 14篇 |
| 2020年 | 6篇 |
| 2019年 | 6篇 |
| 2018年 | 3篇 |
| 2017年 | 5篇 |
| 2016年 | 8篇 |
| 2015年 | 4篇 |
| 2014年 | 5篇 |
| 2013年 | 7篇 |
| 2012年 | 4篇 |
| 2011年 | 8篇 |
| 2010年 | 3篇 |
| 2009年 | 4篇 |
| 2008年 | 4篇 |
| 2007年 | 8篇 |
| 2006年 | 3篇 |
| 2005年 | 1篇 |
| 2004年 | 2篇 |
| 2003年 | 1篇 |
| 2002年 | 1篇 |
| 2001年 | 2篇 |
| 2000年 | 1篇 |
| 1998年 | 2篇 |
| 1995年 | 1篇 |
| 1994年 | 1篇 |
| 1986年 | 1篇 |
| 1985年 | 1篇 |
| 1981年 | 1篇 |
| 1968年 | 1篇 |
| 1956年 | 1篇 |
排序方式： 共有133条查询结果，搜索用时 0 毫秒
91.
M. Cristina Tanese Bruno Pignataro Donato Colangiuli Livia Giotta Francesco Marinelli Francesco Babudri Francesco Naso Luigia Sabbatini P. Giorgio Zambonin Luisa Torsi 《Thin solid films》2008,516(10):3263-3269
The correlations between morphological features and field-effect properties of poly(alkoxyphenylene-thiophene) thin Langmuir-Schäfer film deposited on differently terminated gate dielectric surfaces, namely bare and methyl functionalized thermal silicon dioxide (t-SiO2), have been systematically studied. The film morphology has been investigated at different film thickness by Scanning Force Microscopy. Films thicker than a few layers show comparable morphology on both dielectric surfaces while differences are seen for the ultra-thin polymer deposit in close proximity to the substrate. Such deposit is notably more heterogeneous on bare t-SiO2, while a more compact and uniform nanogranular structure is observed on the silylated t-SiO2. As to the field-effect properties, the methyl-terminated gate dielectric surface leads to a two order of magnitude mobility enhancement along with a field-effect thickness independent conductance. 相似文献
92.
Carolina Reis Guimarães Ilson Paranhos Pasqualino Fabio Faria da Mota Mateus Gomes de Godoy Lucy Seldin Livia Vieira Araujo de Castilho Denise Maria Guimarães Freire 《Journal of surfactants and detergents》2019,22(3):451-462
The use of surfactin, a powerful biosurfactant, is generally hampered by poor production yield. Consequently, identification of new producers and the study of operational parameters are essential. We identify Bacillus sp. H2O-1 as Bacillus velezensis, a species previously not investigated for its biosurfactant production. Among the nitrogen sources we tested, (NH4)2SO4 and NH4NO3 were the most appropriate for surfactin production, reaching 608.5 and 659.5 mg L−1, respectively. Only temperature affected the production, whereas rotation and the C/N ratio did not. Biosurfactants can be used in enhanced oil recovery (EOR) in reservoirs located in the presalt and postsalt layers, where conditions of temperature, pressure, and salinity are quite varied, requiring a study of the stability of these molecules under these conditions. We found the surfactin produced by B. velezensis to be stable at different temperatures, pH, and ionic strengths. We evaluated the concurrent effects of different salinity, temperature, and pressure conditions on surface and interfacial activities of this surfactin. Overall, we found the surfactin produced by B. velezensis H2O-1 to have considerable potential for industrial applications, mainly due to the stability of its physical and chemical characteristics when subjected to different temperatures, pressures, and salinities, in addition to its low toxicity. 相似文献
93.
94.
Chiara Urbinati Livia Cosentino Elena Angela Pia Germinario Daniela Valenti Daniele Vigli Laura Ricceri Giovanni Laviola Carla Fiorentini Rosa Anna Vacca Alessia Fabbri Bianca De Filippis 《International journal of molecular sciences》2021,22(13)
Rett syndrome (RTT) is a rare neurological disorder caused by mutations in the X-linked MECP2 gene and a major cause of intellectual disability in females. No cure exists for RTT. We previously reported that the behavioural phenotype and brain mitochondria dysfunction are widely rescued by a single intracerebroventricular injection of the bacterial toxin CNF1 in a RTT mouse model carrying a truncating mutation of the MeCP2 gene (MeCP2-308 mice). Given the heterogeneity of MECP2 mutations in RTT patients, we tested the CNF1 therapeutic efficacy in a mouse model carrying a null mutation (MeCP2-Bird mice). CNF1 selectively rescued cognitive defects, without improving other RTT-related behavioural alterations, and restored brain mitochondrial respiratory chain complex activity in MeCP2-Bird mice. To shed light on the molecular mechanisms underlying the differential CNF1 effects on the behavioural phenotype, we compared treatment effects on relevant signalling cascades in the brain of the two RTT models. CNF1 provided a significant boost of the mTOR activation in MeCP2-308 hippocampus, which was not observed in the MeCP2-Bird model, possibly explaining the differential effects of CNF1. These results demonstrate that CNF1 efficacy depends on the mutation beared by MeCP2-mutated mice, stressing the need of testing potential therapeutic approaches across RTT models. 相似文献
95.
Stefania Stella Michele Massimino Livia Manzella Maria Stella Pennisi Elena Tirr Chiara Romano Silvia Rita Vitale Adriana Puma Cristina Tomarchio Sandra Di Gregorio Giuseppe Alberto Palumbo Paolo Vigneri 《International journal of molecular sciences》2021,22(2)
Hypereosinophilia (HE) is a heterogeneous condition with a persistent elevated eosinophil count of >350/mm3, which is reported in various (inflammatory, allergic, infectious, or neoplastic) diseases with distinct pathophysiological pathways. HE may be associated with tissue or organ damage and, in this case, the disorder is classified as hypereosinophilic syndrome (HES). Different studies have allowed for the discovery of two major pathogenetic variants known as myeloid or lymphocytic HES. With the advent of molecular genetic analyses, such as T-cell receptor gene rearrangement assays and Next Generation Sequencing, it is possible to better characterize these syndromes and establish which patients will benefit from pharmacological targeted therapy. In this review, we highlight the molecular alterations that are involved in the pathogenesis of eosinophil disorders and revise possible therapeutic approaches, either implemented in clinical practice or currently under investigation in clinical trials. 相似文献
96.
Chiara Romano Federica Martorana Maria Stella Pennisi Stefania Stella Michele Massimino Elena Tirr Silvia Rita Vitale Sandra Di Gregorio Adriana Puma Cristina Tomarchio Livia Manzella 《International journal of molecular sciences》2021,22(14)
Thyroid cancer is the most common malignancy of the endocrine system, encompassing different entities with distinct histological features and clinical behavior. The diagnostic definition, therapeutic approach, and follow-up of thyroid cancers display some controversial aspects that represent unmet medical needs. Liquid biopsy is a non-invasive approach that detects and analyzes biological samples released from the tumor into the bloodstream. With the use of different technologies, tumor cells, free nucleic acids, and extracellular vesicles can be retrieved in the serum of cancer patients and valuable molecular information can be obtained. Recently, a growing body of evidence is accumulating concerning the use of liquid biopsy in thyroid cancer, as it can be exploited to define a patient’s diagnosis, estimate their prognosis, and monitor tumor recurrence or treatment response. Indeed, liquid biopsy can be a valuable tool to overcome the limits of conventional management of thyroid malignancies. In this review, we summarize currently available data about liquid biopsy in differentiated, poorly differentiated/anaplastic, and medullary thyroid cancer, focusing on circulating tumor cells, circulating free nucleic acids, and extracellular vesicles. 相似文献
97.
Xiaotian Jiang Rabab Rashwan Valentina Voigt Jeanne Nerbonne David M. Hunt Livia S. Carvalho 《International journal of molecular sciences》2021,22(9)
Cone Dystrophy with Supernormal Rod Response (CDSRR) is a rare autosomal recessive disorder leading to severe visual impairment in humans, but little is known about its unique pathophysiology. We have previously shown that CDSRR is caused by mutations in the KCNV2 (Potassium Voltage-Gated Channel Modifier Subfamily V Member 2) gene encoding the Kv8.2 subunit, a modulatory subunit of voltage-gated potassium (Kv) channels. In a recent study, we validated a novel mouse model of Kv8.2 deficiency at a late stage of the disease and showed that it replicates the human electroretinogram (ERG) phenotype. In this current study, we focused our investigation on young adult retinas to look for early markers of disease and evaluate their effect on retinal morphology, electrophysiology and immune response in both the Kv8.2 knockout (KO) mouse and in the Kv2.1 KO mouse, the obligate partner of Kv8.2 in functional retinal Kv channels. By evaluating the severity of retinal dystrophy in these KO models, we demonstrated that retinas of Kv KO mice have significantly higher apoptotic cells, a thinner outer nuclear cell layer and increased activated microglia cells in the subretinal space. Our results indicate that in the murine retina, the loss of Kv8.2 subunits contributes to early cellular and physiological changes leading to retinal dysfunction. These results could have potential implications in the early management of CDSRR despite its relatively nonprogressive nature in humans. 相似文献
98.
99.
Marco Lo Presti Maria Michela Giangregorio Roberta Ragni Livia Giotta Maria Rachele Guascito Roberto Comparelli Elisabetta Fanizza Roberto R. Tangorra Angela Agostiano Maria Losurdo Gianluca M. Farinola Francesco Milano Massimo Trotta 《Advanced Electronic Materials》2020,6(7)
A fabrication strategy of photoactive biohybrid electrodes based on the immobilization of the bacterial reaction center (RC) onto indium tin oxide (ITO) is proposed. The RC is an integral photoenzyme that converts photons into stable charge‐separated states with a quantum yield close to one. The photogenerated electron–hole pair can be eventually exploited, with suitable redox mediators, to produce photocurrents. To this purpose, RC must be effectively anchored on the electrode surface and simple strategies for its stable immobilization ensuring prolonged enzyme photoactivity are strongly desired. In this work, polydopamine (PDA), a polymer reminiscent of the natural melanin, is used to anchor the RC on the electrode surface. PDA is easily prepared in situ by spontaneous polymerization of dopamine in slightly alkaline aerated buffered RC solution. This reaction, carried out in the presence of an ITO substrate dipped into the solution, directly leads to a stable RC‐PDA/ITO photoelectrode with 20 nm film thickness and 50% of fully functional RC occupancy. Photocurrents densities recorded using this photoelectrode are comparable to those obtained with far more sophisticated immobilization techniques. The RC‐PDA films are fully characterized by visible–near‐infrared absorption spectroscopy, ellipsometry, atomic force, and scanning electron microscopies. 相似文献
100.