New negative-resistance device by a chirp superlattice

A new type of supperlattice named CHIRP (coherent heterointerfaces for reflection and penetration) superlattice, in which the periodicity is gradually changed, is proposed. The electron transmissivity through the superlattice is modulated by the applied electric field. A possible application to a negative-resistance device is discussed.     

Design and synthesis of Mg2+-selective fluoroionophores based on a coumarin derivative and application for Mg2+ measurement in a living cell

Novel Mg2+ fluorescent molecular probes (KMG-20-AM and KMG-27-AM; where AM is an acetoxymethyl group) based on a coumarin possessing a charged beta-diketone structure were designed and synthesized. These fluorescent probes produced a red shift from 425 to 445 nm in the absorption spectra after formation of a complex with Mg2+. The fluorescence spectra of these probes also showed a red shift from 485 to 495 nm and an increasing fluorescence intensity after formation of a complex with Mg2+. The optimum experimental conditions were excitation wavelength of 445 nm and a monitored wavelength of 500 nm, where these probes functioned as an indicator showing an image of increasing fluorescence in the presence of Mg2+. These probes showed a "seesaw-type" fluorescent spectral change with the isosbestic point at 480 nm due to the light excitation at 445 nm, which indicates that ratiometry can be used for the measurement. The molecular probes formed a 1:1 complex with Mg2+ and the dissociation constant (Kd) was 10.0 mM for KMG-20. The association constants of the probes with Mg2- were approximately 3 times higher than that with Ca2+, which showed that the selectivity of Mg2+ versus Ca2+ for these probes was over 200 times higher than that for commercially available Mg2+ fluorescent molecular probes such as mag-fura-2, Magnesium Green. As an application of these probes, intracellular fluorescent imaging of Mg2+ was demonstrated using a fluorescent microscope. After the addition of KMG-20-AM and KMG-27-AM into PC12 cells, a strong fluorescence was observed in the cytoplasm and a weak fluorescence in the nuclei region. After treatment with a high-K+ medium, the fluorescence intensity increased due to increasing intracellular Mg2+. The real image of Mg2+ release from the magnesium store was successfully observed with these Mg2+ fluorescent probes.     

Optical circulator for an in-line-type compact lidar

The application of an optical circulator is demonstrated for an in-line-type lidar. The lidar's transmitter and receiver are installed in a telescope. The optical circulator of interest here can separate the transmitting laser beam and the echo lights on the same optical axis. It can also divide the echo lights simultaneously into orthogonally polarized components. An insertion loss of 2.2 dB and isolation of >60 dB for the developed optical circulator are obtained in a laser-transmitting situation. This optical circulator makes it possible to measure the polarization ratio caused by cloud phases with a narrow field of view in an in-line-type lidar operation.     

Low voltage FE-STEM for characterization of state-of-the-art silicon SRAM

Low voltage (30 kV) field emission scanning transmission electron microscopy (FE-STEM) has been employed in the characterization of state-of-the-art semiconductor static random access memory (SRAM) using specimens prepared at several different thicknesses (70-180 nm). A focused ion beam (FIB) system, a FIB-SEM compatible specimen holder and an in-lens FE-SEM have been employed for alternating between FIB milling and SEM/ STEM imaging. As a result, ion implanted atom damage during manufacturing, grains in aluminium interconnects, poly silicon gates, thin metal barriers and a thin gate oxide layer were observed by low voltage FE-STEM. STEM, in-lens FESEM, FIB     

Discovery and Evaluation of Aggregate Usage Profiles for Web Personalization

Web usage mining, possibly used in conjunction with standard approaches to personalization such as collaborative filtering, can help address some of the shortcomings of these techniques, including reliance on subjective user ratings, lack of scalability, and poor performance in the face of high-dimensional and sparse data. However, the discovery of patterns from usage data by itself is not sufficient for performing the personalization tasks. The critical step is the effective derivation of good quality and useful (i.e., actionable) aggregate usage profiles from these patterns. In this paper we present and experimentally evaluate two techniques, based on clustering of user transactions and clustering of pageviews, in order to discover overlapping aggregate profiles that can be effectively used by recommender systems for real-time Web personalization. We evaluate these techniques both in terms of the quality of the individual profiles generated, as well as in the context of providing recommendations as an integrated part of a personalization engine. In particular, our results indicate that using the generated aggregate profiles, we can achieve effective personalization at early stages of users' visits to a site, based only on anonymous clickstream data and without the benefit of explicit input by these users or deeper knowledge about them.     

Basic subsite theory assumptions may not be applicable to hydrolysis of cellooligosaccharides by almond beta-glucosidase

Steady-state kinetic parameters for the hydrolysis of cellooligosaccharides by almond beta-glucosidase were evaluated at pH 5.0 and 25 degrees C in relation to the subsite theory (K. Hiromi, Biochem. Biophys. Res. Commun., 40, 1-6, 1970). The value of k0/Km decreased monotonously with increasing degree of polymerization (DP) of the substrates (DP = 2-6). Also, the Km and k0 values for cellotriose were smaller than those for cellobiose. These DP dependencies differ from those of most amylases and glucosidases studied so far, to which the subsite theory has been successfully applied. The subsite parameters could not be consistently obtained, which suggests that one or both of the two basic assumptions of the subsite theory might not be applicable to the hydrolysis of cellooligosaccharides by the enzyme. That is, the intrinsic rate of the hydrolysis may depend on the DP and/or there may be interaction between subsites for binding the glucose residues of a substrate.