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21.
Environmental scanning electron microscopy (ESEM) was used to study the microstructural changes that take place during the hydration of tricalcium aluminate (C3A) in the absence and presence of gypsum (CS¯H2; where A = Al2O3, C = CaO, H = H2O, S¯ = SO3). The ESEM proves to be a valuable tool in the observation of cement hydration and no specialised equipment other than the ESEM is required. The hydration process can be observed at any time without the need to halt the hydration process prior to specimen preparation. Subsequently, artefacts associated with specimen preparation, such as water loss and desiccation, are now avoided. In the absence of sulphate, amorphous gel, poorly crystalline hexagonal calcium aluminate hydrate (? C4AH19) and cubic calcium aluminate hydrate (C3AH6) are observed on the surface of C3A grains. When small amounts of sulphate (2% gypsum) are present the same phases are observed. If larger amounts of sulphate (25% gypsum) are added to the system amorphous gel products, crystalline ettringite (C6AS¯3H32) and monosulphate (C4AS¯H12) are observed. The crystalline products grow both within the amorphous gel and, where space allows, in interstices suggesting a through solution mechanism of transport. 相似文献
22.
Joshua Elliott Meredith Franklin Ian Foster Todd Munson Margaret Loudermilk 《Computational Economics》2012,39(3):219-241
While computable general equilibrium (CGE) models are a well-established tool in economic analyses, it is often difficult
to disentangle the effects of policies of interest from that of the assumptions made regarding the underlying calibration
data and model parameters. To characterize the behavior of a CGE model of carbon output with respect to two of these assumptions,
we perform a large-scale Monte Carlo experiment to examine its sensitivity to base year calibration data and elasticity of
substitution parameters in the absence of a policy change. By examining a variety of output variables at different levels
of economic and geographic aggregation, we assess how these forms of uncertainty impact the conclusions that can be drawn
from the model simulations. We find greater sensitivity to uncertainty in the elasticity of substitution parameters than to
uncertainty in the base-year data as the projection period increases. While many model simulations were conducted to generate
large output samples, we find that few are required to capture the mean model response of the variables tested. However, characterizing
standard errors and empirical probability distribution functions is not possible without a large number of simulations. 相似文献
23.
ABSTRACT Concern over increasing water scarcity has led to the introduction of the concept of agricultural water productivity and an emphasis on interventions to achieve ‘more crop per drop’. Yet, a strong debate continues on how the concept is to be defined and used. Drawing largely from the irrigation literature, the origins of the concept and its methodological developments are reviewed, and its use in applied work over two decades is discussed. Based on this analysis of conceptual and applied research, key insights into the concept’s contributions and limitations are presented, as well as opportunities for further refinements. 相似文献
24.
25.
Yiping Cao Laurie C. Van De Werfhorst Elizabeth A. Scott Meredith R. Raith Patricia A. Holden John F. Griffith 《Water research》2013
Terminal restriction fragment length polymorphism (TRFLP) is an attractive community analysis method for microbial source tracking (MST) because it is accessible, relatively inexpensive, and can discern multiple fecal sources simultaneously. A new Bacteroidales TRFLP (Bac-TRFLP) method was developed and its source identification performance was evaluated by itself, in comparison to, and in combination with an existing universal bacterial TRFLP method in two laboratories. Sixty-four blind samples from 12 fecal sources (sewage, septage, human, dog, horse, cow, deer, pig, chicken, goose, pigeon, and gull) were used for evaluation. Bac- and Univ-TRFLP exhibited similarly high overall correct identification (>88% and >89%, respectively), excellent specificity regardless of fecal sources, variable sensitivity depending on the source, and stable performance across two laboratories. Compared to Univ-TRFLP, Bac-TRFLP had better sensitivity and specificity with horse, cow, and pig fecal sources but was not suited for certain avian sources such as goose, gull, and pigeon. Combining the general and more targeted TRFLP methods (Univ&Bac-TRFLP) achieved higher overall correct identification (>92%), higher sensitivity and specificity metrics, and higher reproducibility between laboratories. Our results suggest that the Bac-TRFLP and Univ&Bac-TRFLP methods are promising additions to the MST toolbox and warrant further evaluation and utilization in field MST applications. 相似文献
26.
Christopher D. Sinigalliano Jared S. Ervin Laurie C. Van De Werfhorst Brian D. Badgley Elisenda Ballesté Jakob Bartkowiak Alexandria B. Boehm Muruleedhara Byappanahalli Kelly D. Goodwin Michèle Gourmelon John Griffith Patricia A. Holden Jenny Jay Blythe Layton Cheonghoon Lee Jiyoung Lee Wim G. Meijer Rachel Noble Meredith Raith Hodon Ryu Michael J. Sadowsky Alexander Schriewer Dan Wang David Wanless Richard Whitman Stefan Wuertz Jorge W. Santo Domingo 《Water research》2013
Here we report results from a multi-laboratory (n = 11) evaluation of four different PCR methods targeting the 16S rRNA gene of Catellicoccus marimammalium originally developed to detect gull fecal contamination in coastal environments. The methods included a conventional end-point PCR method, a SYBR® Green qPCR method, and two TaqMan® qPCR methods. Different techniques for data normalization and analysis were tested. Data analysis methods had a pronounced impact on assay sensitivity and specificity calculations. Across-laboratory standardization of metrics including the lower limit of quantification (LLOQ), target detected but not quantifiable (DNQ), and target not detected (ND) significantly improved results compared to results submitted by individual laboratories prior to definition standardization. The unit of measure used for data normalization also had a pronounced effect on measured assay performance. Data normalization to DNA mass improved quantitative method performance as compared to enterococcus normalization. The MST methods tested here were originally designed for gulls but were found in this study to also detect feces from other birds, particularly feces composited from pigeons. Sequencing efforts showed that some pigeon feces from California contained sequences similar to C. marimammalium found in gull feces. These data suggest that the prevalence, geographic scope, and ecology of C. marimammalium in host birds other than gulls require further investigation. This study represents an important first step in the multi-laboratory assessment of these methods and highlights the need to broaden and standardize additional evaluations, including environmentally relevant target concentrations in ambient waters from diverse geographic regions. 相似文献
27.
Julie I. Jay Shetha Shukair Kristofer Langheinrich Melissa C. Hanson Gianguido C. Cianci Todd J. Johnson Meredith R. Clark Thomas J. Hope Patrick F. Kiser 《Advanced functional materials》2009,19(18):2969-2977
Materials that respond to physiological stimuli are important in developing advanced biomaterials for modern therapies. The reversibility of covalent crosslinks formed by phenylboronate (PBA) and salicylhydroxamate (SHA) has been exploited to provide a pH‐responsive gel for application to the vaginal tract. Dynamic rheology reveals that the gel frequency‐dependent viscoelastic properties are modulated by pH. At pH 4.8 the viscous component dominates throughout most of the frequency range. As the pH increases, the characteristic relaxation time continues to increase while the G′Plateau levels off above pH 6. At pH 7.5, the elastic component dominates throughout the frequency sweep and is predominately independent of frequency. Particle tracking assesses the transport of both fluorescently labeled HIV‐1 and 100‐nm latex particles in the PBA–SHA crosslinked gel as a function of pH. At pH 4.8 the ensemble‐averaged mean squared displacement at lag times greater than three seconds reveals that transport of the HIV‐1 and 100‐nm particles becomes significantly impeded by the matrix, exhibiting diffusion coefficients less than 0.0002 µm2 s?1. This pH‐responsive gel thus displays properties that have the potential to significantly reduce the transport of HIV‐1 to susceptible tissues and thus prevent the first stage of male‐to‐female transmission of HIV‐1. 相似文献
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29.
BACKGROUND: ‘Click’ chemistry, or the 1,3‐dipolar cycloaddition of organic azides with alkynes, has been evaluated for many biomedical purposes; however, its utility in crosslinking hydrogels in situ is limited by the toxicity of the requisite copper(I) catalyst. We report the first use of catalyst‐free Huisgen cycloaddition to generate crosslinked hydrogels under physiological conditions using multivalent azide‐functionalized polymers and an electron‐deficient dialkyne crosslinker. RESULTS: Water‐soluble azide‐functionalized polymers were crosslinked with an electron‐deficient dialkyne crosslinker to form hydrogels at physiological temperature without the addition of copper(I) catalyst. Crosslinking was confirmed using scanning electron microscopy, Fourier transform infrared and 1H NMR analyses. Flow by vial inversion and dynamic rheological methodologies were implemented to evaluate gelation kinetics at 37 °C of variable polymer compositions, concentrations and stoichiometric ratios. Kinetic studies revealed gelation in as little as 12 h at 37 °C, although strong gels that withstand inversion were observed by 1–8 days. CONCLUSION: The ability to form hydrogel networks under mild conditions demonstrates the potential viability of the catalyst‐free ‘click’ crosslinking chemistry for in situ gelling and other biological applications. Further chemical modifications in the crosslinking moieties, as well as polymer and crosslinker conformations, are expected to enhance gelation kinetics to a more biomedically practical rate. Copyright © 2009 Society of Chemical Industry 相似文献
30.
Gallo David A.; Shahid Kameron R.; Olson Meredith A.; Solomon Todd M.; Schacter Daniel L.; Budson Andrew E. 《Canadian Metallurgical Quarterly》2006,20(6):625
Alzheimer's disease (AD) reduces associative effects on false recognition in the Deese-Roediger-McDermott task, either due to impaired memory for gist or impaired use of gist in memory decisions. Gist processes were manipulated by blocking or mixing studied words according to their associations and by varying the associative strength between studied and nonstudied words at test. Both associative blocking and associative strength had smaller effects on false recognition in AD patients than in control participants, consistent with gist memory impairments. However, unlike the case with control participants, blocking influenced true and false recognition equally in AD patients, demonstrating an overdependence on gist when making memory decisions. AD also impaired item-specific recollections, relative to control participants, as true recognition of studied words was reduced even when the two groups were equated on gist-based false recognition. We propose that the overdependence on degraded gist memory in AD is caused by even larger impairments in item-specific recollections. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献