Formation of environmentally relevant brominated dioxins from 2,4,6,-tribromophenol via bromoperoxidase-catalyzed dimerization

Polybrominated dibenzo-p-dioxins (PBDD) are emerging environmental pollutants with structural similarities to the highly characterized toxicants polychlorinated dibenzo-p-dioxins. The geographical and temporal variations of PBDD in biota samples from the Baltic Sea do not display features that are normally related to anthropogenic sources such as incineration, and therefore the natural formation of PBDDs has been suggested. This study of the bromoperoxidase mediated oxidative coupling of 2,4,6-tribromophenol (an abundant substance that is naturally formed in marine systems) identified the formation of ppb-level yields of 1,3,6,8-tetrabromodibenzo-p-dioxin (1,3,6,8-TeBDD) through direct condensation. Additional TeBDDs (1,3,7,9-TeBDD, 1,2,4,7-TeBDD, and/or 1,2,4,8-TeBDD) and tri-BDDs (1,3,7-TrBDD and 1,3,8-TrBDD) were frequently formed but at lower yields. The formation of these TeBDDs probably proceeds via bromine shifts or Smiles rearrangements, whereas the TrBDDs may result from subsequent debromination processes. Because all of the congeners formed by oxidative coupling and subsequent reactions are also found in Baltic Sea biota, the results support the theory that PBDDs are formed from natural precursors.     

Synergistic control of mesenchymal stem cell differentiation by nanoscale surface geometry and immobilized growth factors on TiO2 nanotubes

The aim of this study is to elucidate whether combined environmental signals provided by nanoscale topography and by growth factors control cell behavior of mesenchymal stem cells (MSCs) in a synergistic or simply additive manner. Chondrogenic and osteogenic differentiation of MSCs is studied on vertically aligned TiO(2) nanotubes of size 15 and 100 nm with and without immobilized bone morphogenetic protein-2 (BMP-2). Although BMP-2 coating stimulates both chondrogenic and osteogenic differentiation of MSCs, the response strongly depends on the surface nanoscale geometry of the BMP-2-coated nanotubes. Chondrogenic differentiation is strongly supported on 100 nm BMP-2-coated nanotubes, but not on 15 nm nanotubes, which induce spreading and de-differentiation of chondrocytes. A similar response is observed with primary chondrocytes, which maintain their chondrogenic phenotype on BMP-2-coated 100 nm nanotubes, but de-differentiate on 15 nm nanotubes. In contrast, osteogenic differentiation is greatly enhanced on 15 nm but not on 100 nm BMP-2-coated nanotubes as shown previously. Furthermore, covalent immobilization of BMP-2 rescues MSCs from apoptosis occurring on uncoated 100 nm TiO(2) nanotube surfaces. Thus, combined signals provided by BMP-2 immobilized to a defined lateral nanoscale spacing geometry seem to contain environmental cues that are able to modulate a lineage-specific decision of MSC differentiation and cell survival in a synergistic manner.     

Are Changes in the Percentage of Specific Leukocyte Subpopulations Associated with Endogenous DNA Damage Levels in Testicular Cancer Patients?

Chemoresistance of germ cell tumors (GCTs) represents an intensively studied property of GCTs that is the result of a complicated multifactorial process. One of the driving factors in this process is the tumor microenvironment (TME). Intensive crosstalk between the DNA damage/DNA repair pathways and the TME has already been reported. This study aimed at evaluating the interplay between the immune TME and endogenous DNA damage levels in GCT patients. A cocultivation system consisting of peripheral blood mononuclear cells (PBMCs) from healthy donors and GCT cell lines was used in an in vitro study. The patient cohort included 74 chemotherapy-naïve GCT patients. Endogenous DNA damage levels were measured by comet assay. Immunophenotyping of leukocyte subpopulations was performed using flow cytometry. Statistical analysis included data assessing immunophenotypes, DNA damage levels and clinicopathological characteristics of enrolled patients. The DNA damage level in PBMCs cocultivated with cisplatin (CDDP)-resistant GCT cell lines was significantly higher than in PBMCs cocultivated with their sensitive counterparts. In GCT patients, endogenous DNA damage levels above the cutoff value were independently associated with increased percentages of natural killer cells, CD16-positive dendritic cells and regulatory T cells. The crosstalk between the endogenous DNA damage level and specific changes in the immune TME reflected in the blood of GCT patients was revealed. The obtained data contribute to a deeper understanding of ongoing interactions in the TME of GCTs.     

Experimental investigation of mineral diesel fuel, GTL fuel, RME and neat soybean and rapeseed oil combustion in a heavy duty on-road engine with exhaust gas aftertreatment

The effects of mineral diesel fuel, gas-to-liquid fuel, rapeseed methyl ester, neat soybean and neat rapeseed oil on injection, combustion, efficiency and pollutant emissions have been studied on a compression ignition heavy duty engine operated near full load and equipped with a combined exhaust gas aftertreatment system (oxidation catalyst, particle filter, selective catalytic NO_x reduction). In a first step, the engine calibration was kept constant for all fuels which led to differences in engine torque for the different fuels. In a second step, the injection duration was modified so that all fuels led to the same engine torque. In a third step, the engine was recalibrated in order to keep the NO_x emissions at an equal level for all fuels (injection pressure, injection timing, EGR rate). The experiments show that the critical NO_x emissions were higher (even behind the exhaust gas aftertreatment systems) for oxygenated fuels in case of the engine not being recalibrated for the fuel. GTL and the oxygenated fuels show lower emissions for some pollutants and higher efficiency after recalibration to equal NO_x levels.     

Reevaluating encoding-capacity limitations as a cause of the attentional blink.

A number of researchers have emphasized the role of distractors intervening between successive targets as the primary determinant of the attentional blink (AB) phenomenon. They argued that the AB is abolished when 3 or more targets are displayed as temporally contiguous items in rapidly presented serial sequences. In 3 experiments, the authors embedded 1-, 2-, or 3-digit targets among letter distractors in rapidly presented visual sequences. Across the experiments, both the number of targets and the lag between them were manipulated, producing different proportion of trials in which 3 temporally contiguous targets were presented in the test session. Evidence of an AB affecting the targets that followed the first target in these sequences was found in each experiment when the probability of a given target report was conditionalized on a correct response to the preceding targets, thus reinforcing the notion that some form of capacity limitation in the encoding of targets plays a central role in the elicitation and modulation of the AB effect. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

SATU86 — Simulationsrahmen zur dynamischen Prozeßsimulation für den industriellen Einsatz

Simulation framework for dynamic process simulation for industrial purposes. After a brief treatment of the general requirements of simulation software, the article presents the simulation program SATU86 developed at Hoechst AG. SATU86 permits modular model construction, has a clear user surface and allows a wide range of simulations and identifications, with facilities of tabular and graphic output both during and after simulation. An example of a project serves to illustrate the essential characteristics of SATU86. Apart from development of calculational models in SATU86 the main uses are identification, simulation, and presentation of results.     

What types of word alignment improve statistical machine translation?

In most statistical machine translation (SMT) systems, bilingual segments are extracted via word alignment. However, there is a need for systematic study as to what alignment characteristics can benefit MT under specific experimental settings such as the type of MT system, the language pair or the type or size of the corpus. In this paper we perform, in each of these experimental settings, a statistical analysis of the data and study the sample correlation coefficients between a number of alignment or phrase table characteristics and variables such as the phrase table size, the number of untranslated words or the BLEU score. We report results for two different SMT systems (a phrase-based and an n-gram-based system) on Chinese-to-English FBIS and BTEC data, and Spanish-to-English European Parliament data. We find that the alignment characteristics which help in translation greatly depend on the MT system and on the corpus size. We give alignment hints to improve BLEU score, depending on the SMT system used and the type of corpus. For example, for phrase-based SMT, dense alignments are required with larger corpora, especially on the target side, while with smaller corpora, more precise, sparser alignments are better, especially on the source side. Avoiding some long-distance crossing links may also improve BLEU score with small corpora. We take these conclusions into account to modify two types of alignment systems, and get 1 to 1.6 % relative improvements in BLEU score on two held-out corpora, although the improved system is different in each corpus.     

Ab initio studies of complexation of anions to neutral species

The complexation of simple anions (F⁻ and Cl⁻) to different neutral species, anion-coordinating agents, has been studied using electronic structure calculations. The obtained changes in the equilibrium constants for salt dissolution reactions in different typical electrolyte systems are reported. In addition the lithium ion affinities of the obtained anionic complexes have been calculated. Using the present results we discuss strategies for future usage of anion complexing agents and make recommendations of salt and agent combinations for better lithium battery electrolyte performance.     

Evaluating two ways of calculating priorities in requirements hierarchies - An experiment on hierarchical cumulative voting

When developing large-scale software systems, there is often a large amount of requirements present, and they often reside on several hierarchical levels. In most cases, not all stated requirements can be implemented into the product due to different constraints, and the requirements must hence be prioritized. As requirements on different abstraction levels shall not be compared, prioritization techniques that are able to handle multi-level prioritization are needed. Different such techniques exist, but they seem to result in unfair comparisons when a hierarchy is unbalanced. In this paper, an empirical experiment is presented where an approach that compensate for this challenge is evaluated. The results indicate that some form of compensation is preferred, and that the subjects’ preference is not influenced by the amount of information given.