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61.
T Jiang G Sweeney MT Rudolf A Klip A Traynor-Kaplan RY Tsien 《Canadian Metallurgical Quarterly》1998,273(18):11017-11024
Phosphoinositide 3-OH kinases and their products, D-3 phosphorylated phosphoinositides, are increasingly recognized as crucial elements in many signaling cascades. A reliable means to introduce these lipids into intact cells would be of great value for showing the physiological roles of this pathway and for testing the specificity of pharmacological inhibitors of the kinases. We have stereospecifically synthesized di-C8-PIP3/AM and di-C12-PIP3/AM, the heptakis(acetoxymethyl) esters of dioctanoyl- and dilauroylphosphatidylinositol 3,4,5-trisphosphate, in 14 steps from myo-inositol. The ability of these uncharged lipophilic derivatives to deliver phosphatidylinositol 3,4,5-trisphosphate across cell membranes was demonstrated on 3T3-L1 adipocytes and T84 colon carcinoma monolayers. Insulin stimulation of hexose uptake into adipocytes was inhibited by the kinase inhibitor wortmannin and was largely restored by di-C8-PIP3/AM, which had no effect in the absence of insulin. Thus phosphatidylinositol 3,4,5-trisphosphate or a metabolite was necessary but not sufficient for stimulation of hexose transport. In T84 epithelial monolayers, di-C12-PIP3/AM mimicked epidermal growth factor in inhibiting chloride secretion and potassium efflux, suggesting that phosphatidylinositol 3,4, 5-trisphosphate was sufficient to modulate these fluxes and mediate epidermal growth factor's action. 相似文献
62.
63.
Dipl.-Ing.Dr. techn. Franz G. Rammerstorfer Dipl.-Ing. Dr. techn. Rudolf Beer 《Forschung im Ingenieurwesen》1976,42(5):168-172
Zusammenfassung Eine M?glichkeit, ohne Mehraufwand an Material oder Verwendung teurerer Werkstoffe das dynamische Verhalten oder die Tragf?higkeit
von Bauteilen zu verbessern, wird am Beispiel der Grundfrequenz- und Beullaststeigerung von Kreisplatten durch das Einpr?gen
von geeigneten Eigenspannungszust?nden dargelegt. Die Ergebnisse einer Optimierung solcher Eigenspannungsfelder werden dargestellt,
und die M?glichkeit der Erzeugung geeigneter Eigenspannungszust?nde wird an Hand eines Experiments nachgewiesen. 相似文献
64.
The chlorine and oxygen overpotential in dependence on the current density i (A cm?2) and on the temperature in the range of 15–75°C was measured at γ-MnO2 and β-PbO2 electrodes in concentrated water solutions of sodium chloride and perchlorate. From the measured values the experimental activation energy in dependence on overpotential was calculated and, for the temperature of 25°C, the constants of Tafel's equation (a,b) (α, io respectively) were evaluated. 相似文献
65.
Numbers of Lucilia cuprina (Australian sheep blowfly), Chrysomya spp., and Calliphora spp. blowflies caught on sticky traps baited with various synthetic attractants or a standard liver/sodium sulfide attractant in western Queensland were recorded. Numbers of each genus collected were influenced by the composition of the chemical attractants. Attractant mixtures based on 2-mercaptoethanol, indole, butanoic/pentanoic acid, and a sodium sulfide solution gave 5- to 20-fold higher L. cuprina catches than the liver standard. These blends attracted similar numbers of Chrysomya spp. (0.85-2.7x) and fewer Calliphora spp. (0.02-0.2x) compared to the liver standard. These synthetic attractants were more effective and selective for L. cuprina than the standard liver/sodium sulfide attractant, and they can be packaged in controlled-release dispensers to generate constant, prolonged release of the attractant. 相似文献
66.
In current networks, packet losses can occur if routers do not
provide sufficiently large buffers. This paper studies how many
buffers should be provided in a router to eliminate packet losses.
We assume a network router has m incoming queues, each
corresponding to a single traffic stream, and must schedule
at any time on-line from which queue to take the next packet to
send out. To exclude packet losses with a small amount of
buffers, the maximum queue length must be kept low over the entire
scheduling period. We call this new on-line problem the balanced
scheduling problem (BSP). By competitive analysis, we measure the
power of on-line scheduling algorithms to prevent packet losses.
We show that a simple greedy algorithm is
Θ(log m)-competitive which is asymptotically optimal,
while Round-Robin scheduling is not better than
m-competitive, as actually is any deterministic on-line
algorithm for BSP.
We also give a polynomial time algorithm for
solving off-line BSP optimally.
We also study another on-line balancing problem that tries to
balance the delay among the m traffic streams. 相似文献
67.
Hypoxia occurs over large areas in aquatic systems worldwide, and there is growing concern that hypoxia may affect aquatic animals, leading to population decline and changes in community by elimination of sensitive species. For the first time, we report that sublethal levels of hypoxia can significantly increase (+77.4%) malformation in fish embryonic development. Disruption of apoptotic pattern was clearly evident at 24 h post-fertilization, which may be a major cause of malformation. Furthermore, embryonic development was delayed, and balance of sex hormones (testosterone and estradiol) was disturbed during embryonic stages, implicating that subsequent sexual development may also be affected. Overall, our results imply that hypoxia may have a teratogenic effect on fish and delay fish embryonic development, which may subsequently impair species fitness leading to natural population decline. 相似文献
68.
69.
3 ward climate scales were derived from 2 independent studies linking treatment environments as perceived by patients and staff to treatment outcome as assessed by ward dropout, release, and community tenure rates. Patients and staff from 7 large and 8 small Veterans Administration hospital wards completed the Ward Atmosphere Scales. Patients and staff agreed in perceiving wards with high dropout rates as low in involvement, support, order and organization, and program clarity, whereas wards with high release rates were perceived as strongly emphasizing practical orientation, but as relatively "unexpressive." Wards that kept patients out of the hospital longest were seen as emphasizing autonomy and independence, practical orientation, order and organization, and the open expression of feelings, particularly angry feelings. It is concluded that some relatively consistent findings are emerging from studies in this area. (19 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
70.
We propose image processing algorithms for measuring two-dimensional distributions of linear birefringence using a pair of variable retarders. Several algorithms that use between two and five recorded frames allow us to optimize measurements for speed, sensitivity, and accuracy. We show images of asters, which consist of radial arrays of microtubule polymers recorded with a polarized light microscope equipped with a universal compensator. Our experimental results confirm our theoretical expectations. The lowest noise level of 0.036 nm was obtained when we used the five-frame technique and four-frame algorithm without extinction setting. The two-frame technique allows us to increase the speed of measurement with acceptable image quality. 相似文献