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排序方式: 共有497条查询结果,搜索用时 46 毫秒
81.
Masaharu Takeda Hiroyuki Hiraishi Toshikazu Takesako Sumio Tanase Norio Gunge 《Yeast (Chichester, England)》1996,12(3):241-246
The 36K protein attached at the 5′ end of the linear DNA plasmid pGKL2 from the yeast Kluyveromyces lactis was first purified and characterized. The terminal protein was purified from cells (1 kg wet weight) by ammonium sulphate precipitation and two rounds of centrifugation to equilibrium in CsCl gradients. The pGKL2 was present only in the post-microsomal supernatant. Approximately 10 mg of the purified pGKL2 was recovered and digested with DNase I. The terminal protein (final ca. 0·8 mg) was homogeneous by electrophoresis and we determined the N-terminal amino acid sequence up to ten residues, showing that it existed in the cryptic N-terminal domain of pGKL2-ORF2 (DNA polymerase) sequence. 相似文献
82.
Toshikazu Nishiwaki Satoshi Asano Takuji Ohyama 《Journal of Bioscience and Bioengineering》2009,107(6):605-609
Highly active proteolytic enzymes are found in the fruiting bodies of Grifola frondosa. The general properties and substrate specificities of these proteases from G. frondosa (ProGF) were studied. The optimal pH for ProGF activity was pH 3 or 7 using hemoglobin or Hammersten casein as a substrate, respectively. The ProGF exhibited over 70% of maximal activity within the pH range of 4.5–8.5. In terms of temperature, the ProGF were maximally active at 55 °C, while over 80% of maximal activity was observed within the range of 50–75 °C. These proteases were substrate-specific, mainly cleaving at Ala14-Leu15, Tyr16-Leu17, and Pro28-Lys29 bonds, with occasional cleavage of Phe24-Phe25 bonds in the oxidized insulin B-chain. The ProGF also liberated hydrophobic amino acids, such as valine, leucine, and phenylalanine, using the oxidized insulin B-chain as a substrate. When soy protein was used as a substrate, valine, leucine, phenylalanine, and tyrosine were selectively released from the hydrolysate. Thus, over the time course of incubation, the peptide concentration increased as the average peptide chain length decreased. These results indicate that the ProGF include both endopeptidases recognizing leucine, phenylalanine, and lysine at the P1′ position, and aminopeptidases preferentially releasing hydrophobic and aromatic amino acids such as valine, leucine, phenylalanine, and tyrosine. 相似文献
83.
Yamamoto A Kakutani N Yamamoto K Kamiura T Miyakoda H 《Environmental science & technology》2006,40(13):4132-4137
A highly sensitive and uncomplicated method of analyzing steroidal hormones in river and estuarine water samples was developed using a liquid chromatography tandem mass spectrometer equipped with an electrospray ionization (ESI) source and atmospheric pressure photoionization (APPI) source. Steroidal hormones included not only estrogen but also androgen and conjugates of these two. APPI displayed greater sensitivity than ESI for most of the unconjugated steroids examined, with very high sensitivity for testosterone and 4-androstene-3,17-dione in particular. For conjugated hormones, in contrast, ESI was more effective. The method developed was applied to the determination of hormones in the rivers of Osaka City and their estuaries, where the hormones detected were affected by the effluent from municipal wastewater treatment plants (WWTPs), and hormone concentration values were comparable to those reported in previous studies of such effluent. Because of the two-way flow and stagnancy of streams and watercourses, continuous input of steroidal hormones from WWTPs seems to bring about local accumulation. Levels of androgen were 1 order of magnitude lower than those of estrogen. Estrone, estrone 3-sulfate, and 4-androstene-3,17-dione were detected in almost all water samples, with maxima of 51, 5.1, and 6.4 ng L(-1), respectively. 相似文献
84.
85.
Ichiro Yamanaka Toshikazu Hashimoto Ryo Ichihashi Kiyoshi Otsuka 《Electrochimica acta》2008,53(14):4824-4832
Direct synthesis of H2O2 acid solutions was studied using a gas-diffusion cathode prepared from activated carbon (AC), vapor-growing-carbon-fiber (VGCF) and poly-tetra-fluoro-ethylene (PTFE) powders, with a new H2/O2 fuel cell reactor. O2 reduction to H2O2 was remarkably enhanced at the three-phase boundary (O2(g)-electrode(s)-acid(l)) at the [AC + VGCF] cathode. Fast diffusion processes of O2 to the active surface and of H2O2 to the bulk acid solutions were essential for H2O2 accumulation. Synergy of AC and VGCF was observed for the H2O2 formation. RRDE and cyclic voltammetry studies indicated that the surface of AC functioned as the active phase for O2 reduction to HO2, and VGCF functioned as an electron conductor and a promoter to convert HO2 to H2O2. A maximum H2O2 concentration of 353 mM (1.2 wt%) was accomplished under short-circuit conditions (current density 12.7 mA cm−2, current efficiency 40.1%, geometric area of cathode 1.3 cm2, reaction time 6 h). 相似文献
86.
M. Shibata T. Arai A. Fukuda H. Funahashi T. Haseyama S. Ikeda K. Imai Y. Isozumi T. Kato Y. Kido A. Matsubara S. Matsuki T. Mizusaki R. Nakanishi T. Nishimura D. Ohsawa A. Sawada Y. Takahashi M. Tosaki S. Yamada K. Yamamoto 《Journal of Low Temperature Physics》2008,151(3-4):1043-1048
A microwave single-photon detector was developed with highly-excited alkaline Rydberg-atoms in a cooled resonant cavity to
search for dark matter axions. This detector belongs to a microwave single-photon counter, thus being free from the standard
quantum limit (SQL). High sensitivity of the present detector system was demonstrated by measuring the thermal blackbody radiations
in the cavity at temperatures as low as 70 mK where the sensitivity is below the SQL.
The detection sensitivity of the present system is mainly limited by stray electric fields present in the detection region.
Practical design of a new experimental scheme with a guiding electric field through the atomic-beam trajectory is here presented
and discussed to avoid the effect of stray electric field and thus to improve the detection sensitivity.
相似文献
87.
As the density and operating speed of complementary metal oxide semiconductor (CMOS) circuits increases, dynamic power dissipation has become a critical concern in the design and development—of personal information systems and large computers. The reduction of supply voltage, node capacitance, and switching activity are common approaches used in conventional CMOS. In adiabatic switching circuits, the current flow through transistors can be significantly reduced by ensuring uniform charge transfer over the entire available time. This paper presents the simulation of this current in two-phase clocked adiabatic static CMOS logic (2PASCL) and conventional CMOS. From the SPICE simulations, at transition frequencies from 1 to 12 MHz, a 4×4-bit array 2PASCL multiplier shows a maximum reduction in power dissipation of 77% relative to that of a static CMOS. The measurement results of a 4×4-bit array 2PASCL multiplier demonstrate a 57% reduction compared to a 4×4-bit array two-phase clocked adiabatic dynamic CMOS logic (2PADCL). These results indicate that 2PASCL technology can be advantageous when applied to low-power digital devices operated at low frequencies, such as radio-frequency identification (RFID) tags, smart cards, and sensors. 相似文献
88.
Jun-ichi Horinaka Arisa Okamoto Toshikazu Takigawa 《Sensing and Instrumentation for Food Quality and Safety》2016,10(2):220-225
Dynamic viscoelasticity measurements were performed for concentrated solutions of konjac glucomannan in an ionic liquid. The entanglement coupling appeared in the rheological data for each solution was characterized in terms of the molecular weight between entanglements (M e) as an average size of the transient entanglement network. The value of M e for konjac glucomannan in the molten state was estimated to be 1.8 × 103 (in g mol?1), being significantly smaller than that for cellulose, although the molecular weight and linkage of the repeating units were the same between these polysaccharides. This result suggested that the configuration of the repeating monosaccharide unit affected the entanglement network of these polysaccharides reflecting the single chain characteristics. 相似文献
89.
Izumi Satake John H. XinTu Tianming Aran HansuebsaiKenji Ando Tetsuya SatoKanji Kajiwara Seiji Ohsawa 《Progress in Organic Coatings》2011,72(3):528-540
This article compares color assessments of automotive exteriors at four locations in China, Thailand and Japan. This study employed painted panels of twelve colors, thirty sensory words and fifteen pairs of emotion variables. It also used reference figures of nine cars that included four styling categories, namely sedans, compacts, sport utility vehicles and sports cars. The results of like-dislike tests revealed that basic automotive exterior colors, such as black, white and red, were highly preferred across locations. However, geographical differences are apparent in the preference for some non-basic colors. To elucidate these geographical differences, a Chi-square test and principal component analysis were performed on the data on emotion variables. The results revealed both similarities and differences in color preferences among the four locations. Color preferences were similar in Hong Kong and Shanghai when simply evaluated by color samples. However, similar preferences for automotive exterior colors were observed in Hong Kong, Kyoto and Bangkok, but not in Shanghai. These results suggest that there is fluctuation in the emotion evoked by a color when the color is considered in the context of automotive exteriors. Therefore, this study indicates that automotive sales can be secured in the global marketplace by creating a single palette of exterior colors based on similar color preferences across countries. However, these results also indicate that preparing market-specific automotive-color line-ups can be an effective marketing strategy. 相似文献
90.
Toshikazu Okubo Tamie Kodera Kazuo Kondo 《Chemical Engineering Communications》2013,200(12):1503-1513
The authors established a method to ensure uniform patterned copper plating layer thickness in semiconductor packages with high-density electrical circuits or ball grid arrays (BGA). A single BGA pattern unit was divided into small areas and for each, active area density was defined according to the area covered by the copper layer. Current distribution was determined for such an area by numerically solving Laplace and Butler-Voltmer equations, with consideration directed to active area density and plating solution conductivity. With the auxiliary grid electrode placed at a certain distance from the BGA so as to surround the latter by the former electrode, the current was found to be quite effectively prevented from becoming concentrated on sparsely dispersed circuit patterns generally situated near the edge of a unit BGA. Current distribution was noted to vary according to position and cross section size. Error could be made as little as 3.37% when this position was optimal. 相似文献