Genomic studies of milk-related traits in water buffalo (Bubalus bubalis) based on single-step genomic best linear unbiased prediction and random regression models

Genomic selection has been widely implemented in many livestock breeding programs, but it remains incipient in buffalo. Therefore, this study aimed to (1) estimate variance components incorporating genomic information in Murrah buffalo; (2) evaluate the performance of genomic prediction for milk-related traits using single- and multitrait random regression models (RRM) and the single-step genomic best linear unbiased prediction approach; and (3) estimate longitudinal SNP effects and candidate genes potentially associated with time-dependent variation in milk, fat, and protein yields, as well as somatic cell score (SCS) in multiple parities. The data used to estimate the genetic parameters consisted of a total of 323,140 test-day records. The average daily heritability estimates were moderate (0.35 ± 0.02 for milk yield, 0.22 ± 0.03 for fat yield, 0.42 ± 0.03 for protein yield, and 0.16 ± 0.03 for SCS). The highest heritability estimates, considering all traits studied, were observed between 20 and 280 d in milk (DIM). The genetic correlation estimates at different DIM among the evaluated traits ranged from ?0.10 (156 to 185 DIM for SCS) to 0.61 (36 to 65 DIM for fat yield). In general, direct selection for any of the traits evaluated is expected to result in indirect genetic gains for milk yield, fat yield, and protein yield but also increase SCS at certain lactation stages, which is undesirable. The predicted RRM coefficients were used to derive the genomic estimated breeding values (GEBV) for each time point (from 5 to 305 DIM). In general, the tuning parameters evaluated when constructing the hybrid genomic relationship matrices had a small effect on the GEBV accuracy and a greater effect on the bias estimates. The SNP solutions were back-solved from the GEBV predicted from the Legendre random regression coefficients, which were then used to estimate the longitudinal SNP effects (from 5 to 305 DIM). The daily SNP effect for 3 different lactation stages were performed considering 3 different lactation stages for each trait and parity: from 5 to 70, from 71 to 150, and from 151 to 305 DIM. Important genomic regions related to the analyzed traits and parities that explain more than 0.50% of the total additive genetic variance were selected for further analyses of candidate genes. In general, similar potential candidate genes were found between traits, but our results suggest evidence of differential sets of candidate genes underlying the phenotypic expression of the traits across parities. These results contribute to a better understanding of the genetic architecture of milk production traits in dairy buffalo and reinforce the relevance of incorporating genomic information to genetically evaluate longitudinal traits in dairy buffalo. Furthermore, the candidate genes identified can be used as target genes in future functional genomics studies.     

Sensors and observers in distributed parameter systems

Luenberger observer theory is extended to distributed parameter systems. This extension is based on the consideration of sensors. For systems with infinite dimensional state spaces, it is possible to construct the state vector asymptotically (or a part of the state vector) by a ‘good’ choice of sensors. We show that the link between detectability and sensor structure may be of some interest in the construction of observers.     

Production of fungal lipases using wheat bran and soybean bran and incorporation of sugarcane bagasse as a co-substrate in solid-state fermentation

Fungal strains were screened for lipase producing activities and 10 strains were classified as good producers. Aspergillus sp., Fusarium sp., and Penicillium sp. exhibited the highest activities when fermented in wheat bran (WB) and soybean bran (SB). No fungal growth was observed using sugarcane bagasse (CB). An experimental design was applied to incorporate CB into the fermentation process for lipase production by Aspergillus sp. and Penicillium sp., and to evaluate the best moisture content for the substrate. Strains studied achieved maximum lipase activities with 25% CB combined with 75% WB or SB at 40% moisture content. The highest lipase activities were observed for WB and SB, and for SB combined with CB using Aspergillus sp. Fermentation of 96 h was the optimum period for enzyme production.     

ANTIBACTERIAL EFFECT, HISTOLOGICAL IMPACT AND OXIDATIVE STRESS STUDIES FROM LAURUS NOBILIS EXTRACT

Laurus nobilis is one of the most broadly used spices in Moroccan gastronomy. Its antimicrobial activity against both gram-positive and gram-negative bacterial species was demonstrated. Likewise, its toxicity was investigated on Swiss albinos' mice . Daily, mice were treated orally with 0.003 and 0.3 mg during 7 days. Plasmatic markers and enzymatic systems were assessed and histological alterations were evaluated. A significant increase in creatine phosphokinase level was observed. The microscopic evaluation showed that L. nobilis induces morphological perturbation in mice's liver. The results also showed an inhibitory effect of glyceraldehydes 3-phosphate dehydrogenase and an important increase in the level of thiobarbituric acid reactive substances and succinate dehydrogenase and no change in catalase activities. The results showed that L. nobilis induces toxicity at 0.3 mg/g mouse and affects energy metabolism and oxidative stress.

PRACTICAL APPLICATIONS

The aim of the present study is to determine the antimicrobial effect of Laurus nobilis on pathogens for potential application as herbal medicine in infusion or oral preparation. The effect of the spice extract was investigated on the metabolic markers, stress biomarkers and clinical parameters. This study was completed with histological coupes. To our knowledge, this is the first report to demonstrate the laurel effect on energy metabolism and oxidative stress.     

Computation of finite and infinite zeros of linear multivariable systems: a state space approach

An iterative algorithm, developed by the authors to compute the finite zeros of a linear multivariable system (Sebakhy et al. 1983), is extended to compute the infinite zeros of the system as well. The method is simple, efficient and suitable for programming on a digital computer     

Stabilization of controlled positive discrete‐time T‐S fuzzy systems by state feedback control

This paper deals with sufficient conditions of asymptotic stability and stabilization for nonlinear discrete‐time systems represented by a Takagi–Sugeno‐type fuzzy model whose state variables take only nonnegative values at all times t for any nonnegative initial state. This class of systems is called positive systems. The conditions of stabilizability are obtained with state feedback control. This work is based on multiple Lyapunov functions. The results are presented in linear matrix inequalities form. A real plant is studied to illustrate this technique. Copyright © 2010 John Wiley & Sons, Ltd.     

Impact of diesel policy banning on PM levels in urban areas

Increased use of diesel engine in on‐road vehicles presents a serious health concern, particularly in traffic‐congested urban areas. Diesel exhaust contains various gaseous and particulate pollutants, which, at high concentrations, pose adverse health effects. In this respect, various policy measures are being adopted worldwide to curtail emissions from diesel engines. This paper presents an assessment of diesel engine policy banning in the Greater Beirut Area. For this purpose, particulate matter levels in the air were measured after the ban and compared with concentrations reported prior to the ban. Health‐based socio‐economic benefits associated with improvement in air quality were then estimated using the long‐term decrease of particulate matter as an indicator.     

From application service provision to service-oriented computing: A study of the IT outsourcing evolution

IT outsourcing is not a new concept. Originating from the early 60’s in the form of time sharing, IT outsourcing has evolved to the application service provision (ASP) model, in the late 90’s to pure services in the beginning of the century. Services, and their major representative web services, are actually the next phase of the movement toward Internet-based componentized software, known in the not so distant past as ASP. As the continuous expansion of the Internet and its relating technologies creates new marketing opportunities, traditional monolithic architectures are giving way to service-oriented computing (SOC), the architecture that enables service provision. SOC permits the utilization of large systems which are comprised of self-containing building blocks: services. Services may be made public, searched, reused and combined to form complex business processes while in the same time retaining a significant level of flexibility. Services and SOC have emerged as a response to a fundamental shift in enterprise business culture that started at the late 90’s. Although they are promising as an IT outsourcing enabler, many issues need to be dealt with before they can be considered suitable for wide adoption. These issues are of technological, business, economic and cultural nature and they were also faced by ASPs in the past. It is worth revisiting the successes and failures of the ASP model in order to get a better understanding of the evolving IT outsourcing industry. In this work, we describe the evolution of service provision from its initial form as software application, through the application service provision era and towards the new trend of web services.