Failure of soybean trypsin inhibitor to exert deleterious effects in calves

To evaluate nutritional effects induced in calves by feeding soybean trypsin inhibitor, 16 calves were fed 1) raw soybeans, 2) heated soybeans, 3) heated soybeans plus soybean trypsin inhibitor, 4) heated (raw soybeans plus soybean trypsin inhibitor). Ration 1 caused depression of growth and reduced digestibility of protein and fat as compared to Ration 2. No differences were significant in calves fed Rations 3 and 4. The weights and enzymatic activities of pancreas were similar in all groups. Soybean trypsin inhibitor plays a minor role, if any, in calf nutrition.     

The Blalock-Taussig operation: the procedure of choice in the hypoxic infant with tetralogy of Fallot

An experimental model is presented enabling an analysis of the healing process of completely cut and re-sutured free segments of rabbit flexor tendons, kept avascular in a synovial milieu and completely isolated from adhesion formation. Under these conditions the cut tendons heal within a few weeks. It can be shown that this healing process is a result of intrinsic tendon cell activity only.     

Chemical absorption and desorption of carbon dioxide from hot carbonate solutions

Absorption and desorption rate data for the system CO₂-hot carbonate solutions are presented. The data are interpreted on the basis of a film-theory model developed following the procedure recently presented by Astarita and Savage [1]. The agreement is very satisfactory. Values of the kinetic constant of the rate-determining step, previously known only up to a temperature of 40°C, have been obtained up to 110°C.     

Reflection in the systemic arterial system: effects of aortic and carotid occlusion

Experiments were performed in seven closed-chest anaesthetized male dogs to determine the role of pulse wave reflection in the pattern of flow and pressure in the ascending aorta. Ten days after implantation of an electromagnetic flow transducer around the ascending aorta a balloon catheter was placed in the descending aorta via the femoral arteries. At the same time a tip manometer was introduced into the ascending aorta. Aortic occlusions at three different sites caused pressure pulses with secondary systolic rises and flow pulses with biphasic deceleration. Secondary rises occurred 45 +/- 9.0 ms after the initial pressure rise for high aortic occlusion; this time was 75 +/- 8.5 ms for occlusion at the level of the diaphragm and 114 +/- 16.5 ms for occlusion near the level of the renal arteries. These times approximate the times in which the pulse travels from the tip manometer to the inflated balloons and back. Forward and reflected pressure and flow waves were calculated from reflection coefficients. Aortic occlusion caused larger reflected waves and the recorded wave forms were caused by the summation of forward and backward waves, the latter contributing the secondary pressure rise and the increased flow deceleration. Occlusion of both carotid arteries showed no specific reflection site but reflected waves were larger. This increased reflection can probably be explained as the result of greater total reflection from distributed sites under increased peripheral resistance.     

Carrageenin-induced arthritis. III. Proteolytic enzymes present in rabbit knee joints after a single intraarticular injection of carrageenin

A single intraarticular injection of carrageenin into the rabbit knee joint initiates an inflammatory reaction in the synovial tissues. the exudate from the joint was able to degrade proteoglycan at pH 5.2 and pH 7.2. Further characterization of proteolytic enzymes in the inflamed synovial tissues showed the presence of cathepsin D, a neutral protease, and cathepsin B1. Maximum activities of two lysosomal enzymes, acid phosphatase and cathepsin D, were observed within 7 days of injection. Most of this activity was found to be associated with cells in the synovial fluid.     

Elimination of Salmonella in stabilization ponds

While the reduction of putrescible organic matter is important in sewage treatment, the microbiological quality of the effluents is of great public health significance, since these are usually discharged into streams or onto land. The performance of two types of stabilization ponds located at Nagpur, India, was studied with respect to the reduction of Salmonella, Coliforms, E. coli and faecal streptococci. The two ponds were operated at different BOD loading and detention time. Furthermore while one had two cells with submerged interconnection the other had three cells interconnected with a surface overflow arrangement. Comparing the results of these two ponds, treating domestic sewage, it is found that Salmonella does not get eliminated in the two celled pond while they are absent in 1 litre aliquots of effluent samples from the three celled pond. The indicator organisms are reduced to a great extent in the latter pond as compared to the former. From this it appears that the number of cells and the interconnecting arrangements are very important besides the loading, detention period and other design features.     

Evaluation of critical orifices made from sections of hypodermic needles

Levamisole, a potentiator of cell-mediated immunity, has been reported to increase survival in patients with resectable carcinoma of the lung. Cell-mediated immunity can be measured in vitro by the leukocyte migration inhibition test. We have previously reported that this test detects cell-mediated immunity to human lung tumor antigens. In the present studies, patients with lung cancer were treated with Levamisole. Their leukocytes were evaluated in the leukocyte migration inhibition assay before, during, and following Levamisole therapy. Small increases in cell-mediated immunity were observed when patients had a high pre-existing tumor immunity. When tumor-associated reactivity was absent prior to therapy, larger increases were measured. Although Levamisole is a nonspecific immunostimulant, these data indicate that in vitro anti-tumor immune responses are enhanced by Levamisole therapy. Augmentation of cell-mediated immunity to tumor antigens may explain the clinical benefits of Levamisole therapy.