Papillary-cystic neoplasm of the pancreas. A sex-steroid dependent tumor

Respiratory papillomatosis is a rare and often severe disease, usually localized in the larynx. It may cause respiratory distress and even life-threatening obstruction of the airways. Treatment is generally based on the evaporation of the lesions with a CO2 laser, but microsurgery, cytotoxic and/or cytostatic drugs, interferons, and vaccines are also used. Cidofovir [(S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine] (HPMPC) was shown to suppress the growth of tumors induced by rabbit papillomavirus as well as human papillomavirus (HPV). The efficacy of cidofovir was assessed in 17 patients with severe respiratory papillomatosis. Cidofovir at a concentration of 2.5 mg/ml was injected directly in the different laryngeal papillomatous lesions during microlaryngoscopy under general anesthesia. Biopsies were taken before the treatment was started both for anatomopathology and viral typing. HPMPC kinetics in serum was monitored in three patients, the drug levels being determined by high-performance liquid chromatography. Complete disappearance of the papillomatosis was observed in 14 patients. Four patients relapsed and were successfully treated again with cidofovir. Of the three remaining patients, one progressed while under treatment with cidofovir, after an initial marked response. One patient had a partial remission and remained stable for more than 1 year after the last injection. He had a very aggressive and extensive disease originally. Finally, one patient was lost to follow-up after four injections. Intratumoral injections of cidofovir for the treatment of severe laryngeal papillomatosis is a powerful new therapeutic approach for this disease. Treatment was well tolerated, and no significant side effects were noted.     

Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum

Chromosome 2 of Plasmodium falciparum was sequenced; this sequence contains 947,103 base pairs and encodes 210 predicted genes. In comparison with the Saccharomyces cerevisiae genome, chromosome 2 has a lower gene density, introns are more frequent, and proteins are markedly enriched in nonglobular domains. A family of surface proteins, rifins, that may play a role in antigenic variation was identified. The complete sequencing of chromosome 2 has shown that sequencing of the A+T-rich P. falciparum genome is technically feasible.     

Expression and functional role of tumor necrosis factor receptors on leukemic cells from patients with type B chronic lymphoproliferative disorders

Two receptors for tumor necrosis factor (TNF) with different molecular weight (75-Kd and 55-Kd) and binding affinity have been recently discovered. To investigate the distribution and the functional role of these receptors on leukemic B cells from hairy cell leukemia (HCL) and B-cell chronic lymphocytic leukemia (B-CLL) patients, we evaluated: (1) the cytofluorimetric pattern of uncultured and cultured leukemic B cells incubated with utr-1 and htr-9 monoclonal antibodies (MoAbs), which specifically recognize the 75-Kd and 55-Kd TNF receptors (TNFR), respectively; (2) the effect of TNF-alpha and TNF-beta on leukemic B cells in an in vitro proliferation assay; (3) the role of anti-TNFR MoAbs on TNF-alpha and TNF-beta-driven B-cell growth; and (4) the proliferative effect of utr-1 and htr-9 MoAbs on in vitro cultured leukemic cells. Our study shows that the high affinity (75-Kd) but not the low affinity (55-Kd) TNFR molecules are expressed on freshly isolated leukemic B cells recovered from HCL and B-CLL patients. The expression of these receptors was neither upregulated nor downregulated by different stimuli, including TNF-alpha, TNF-beta, B-cell growth factor, and interleukin-2. TNF-alpha efficiently triggers the proliferation of HC and, to a lesser extent, the growth of B-CLL cells. TNF-beta was also able to transduce the proliferative signal in HCL, but not in B-CLL patients. TNF-alpha- and TNF-beta-driven B-cell proliferation was inhibited by the preincubation of leukemic B cells with utr-1 but not htr-9 MoAb. Moreover, anti-75-Kd, but not anti-55-Kd TNFR MoAb, was able to trigger the proliferation of leukemic B cells, and in particular of HC. These results show that leukemic B cells from patients with HCL and B-CLL are equipped with a fully functional high affinity TNFR.     

Neural-network-based blackboard demon subsystems

We propose two neural network architectures involving feedforward and probabilistic neural network models to simulate the blackboard demon subsystem, which is responsible for triggering knowledge sources in accordance with the complex monitoring conditions on the hypotheses in the blackboard. Both architectures involve a preprocessor module to perform the transformation between symbolic hypotheses and numerical ones. They learn and record the triggering relationships between hypotheses and knowledge sources in the network links and perform better blackboard monitoring function than the traditional symbolic demon subsystems. In comparison, the probabilistic neural network-like architecture performs better when there is a possibility of using a centralized knowledge representation and when it only involves one-to-one or one-to-many mapping between hypotheses and triggering patterns. The feedforward architecture may be useful when a distributed knowledge representation is possible and when time requirements for training the architecture to learn the complex mappings are not too strict.     

A new transformation-deficient mutant of Haemophilus influenzae Rd with normal DNA uptake

Haemophilus influenzae Rd is a gram-negative natural transformer. A mutant strain, RJ248, that has normal DNA uptake and translocation but whose transformation frequency is 300 times lower than that of wild-type H. influenzae and whose phage recombination is 8 times lower was isolated. The affected gene, comM, is induced during competence development in wild-type H. influenzae but not in RJ248.     

Risk for endometrial cancer following breast cancer: a prospective study in Sweden

Many marine organisms are luminescent. The proteins that produce the light include a primary light producer (aequorin or luciferase) and often a secondary photoprotein that red shifts the light for better penetration in the ocean. Green fluorescent protein is one such secondary protein. It is remarkable in that it autocatalyzes the formation of its own fluorophore and thus can be expressed in a variety of organisms in its fluorescent form. The recent determination of its 3D structure and other physical characterizations are revealing its molecular mechanism of action.     

Towards photostatistics from photon-number discriminating detectors

Abstract

We study the properties of a photodetector that has a numberresolving capability. In the absence of dark counts, due to its finite quantum efficiency, photodetection with such a detector can only eliminate the possibility that the incident field corresponds to a number of photons less than the detected photon number. We show that such a non-photon number-discriminating detector, however, provides a useful tool in the reconstruction of the photon number distribution of the incident field even in the presence of dark counts.     

Atmospheric correction for the sea surface temperature using NOAA-7 AVHRR and METEOSAT-2 infrared data

The multi-spectral method for atmospheric corrections is investigated. The analysis shows that the non-linear effects of the atmospheric absorption can be accounted for by using three channels. The results clarify and lend theoretical support to previous works. A simple correction procedure is proposed. In the case where channel 3 of the NOAA AVHRR is not available or is contaminated, simultaneous METEOSAT infrared data can be used instead. The result tested with in situ measured temperatures shows a marked improvement when three channels are used. In this procedure, the detailed knowledge of the local atmospheric profiles is not required.     

Discrete pulse orthogonal functions for the analysis,parameter estimation and optimal control of linear digital systems

Discrete pulse orthogonal functions (DPOFs) are introduced, and their operational matrix is proposed to solve various control problems. The applications of DPOFs in digital control systems are analogous to those of block pulse functions (BPFs) in continuous systems. Applying the DPOFs, the approximate solutions of digital time-invariant systems can be easily obtained by a convenient algorithm. Three examples are presented to demonstrate the applications of DPOFs.