Solubilization of fish muscle proteins with buffers containing sodium dodecyl sulfate

Summary The extraction of fish muscle protein using SDS containing solubilization buffers was studied varying the time and the temperature of solubilization, as well as pH and SDS concentration of the buffer. At pH < 6 the myofbrillar proteins were incompletely solubilized; temperatures of 80-100 °C resulted in protein degradation observable in the SDS-PAGE.Samples of fish muscle containing high amounts of formaldehyde (50 mmoles FA/kg wet weight) could only be solubilised at 100 °C; on the other hand it was possible to solubilize cooked and/or canned products under mild conditions (2% SDS, 1% 2-ME, pH 8.9, shaking for 2 h at 60 °C).

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Solubilisierung von Fischmuskelproteinen durch Natriumdodecylsulfathaltige Puffer

Zusammenfassung Der Einfluß von Solubilisierungszeit und -temperatur, des pH-Wertes und der SDS-Konzentration des Solubilisierungspuffers auf die Extraktion von Fischmuskelproteinen wurde überprüft. Bei pH-Werten < 6 wurden die myofbrillären Proteine nur unvollständig gelöst; Solubilisierungstemperaturen von 80 bis 100 °C führten zu einem in der SDS-PAGE sichtbaren Proteinabbau. Fischmuskelproben mit hohem Formaldehydgehalt (50 mmole FA/kg Feuchtgewicht) ließen sich nur bei 100 °C vollständig solubilisieren; demgegenüber gelang die weitgehende Solubilisierung gegarter und/oder sterilisierter Produkte unter milden Bedingungen (2% SDS, 1% 2-ME, pH 8,9; 2stündiges Schütteln bei 60 °C).

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Abbreviations FA formaldehyde - IEF isoelectric focusing - 2-ME 2-mercaptoethanol - PA polyacrylamide - PAGE polyacrylamide gel electrophoresis - pI isoelectric point - SDS sodium dodecyl sulfate - TMAO trimethylamine oxide - Tris Tris (hydroxymethyl)methylamine     

Dietary energy restriction does not inhibit pancreatic carcinogenesis by N-nitrosobis-2-(oxopropyl)amine in the Syrian hamster

Dietary energy restriction was previously shown to be effective in preventing a wide range of experimentally induced cancers. Studies were conducted to assess the influence on pancreatic carcinogenesis of dietary energy restrictions (reduced fat and carbohydrate) of 10%, 20% or 40% in comparison with control in Syrian hamsters treated with N-nitrosobis(2-oxopropyl)amine (BOP). Two carcinogenesis studies were conducted. One used a single treatment with 20 mg BOP/kg body weight and followed hamsters for 102 weeks following treatment, and the other used three weekly treatments of 20 mg BOP/kg body weight and followed hamsters for 45 weeks after treatment. Hamsters were fed control or energy restricted diet beginning the week following the last BOP treatment. Pancreatic carcinomas were induced in 9-18% of the hamsters in the first experiment and in 59-66% of the animals in the second. Dietary energy restriction did not influence carcinoma incidence in either study, and in the second experiment the multiplicity of tumors was higher in the 40% energy restriction (ER) group than in control hamsters. Plasma corticosterone was suppressed by BOP treatment, particularly in the 20% and 40% ER hamsters in the second experiment, and diet or BOP treatment did not significantly alter plasma cortisol. Pancreatic protein kinase Czeta measured by Western blot was highest in the cytosol and particulate fractions of the 40% ER hamsters in the first experiment. These results indicate that dietary energy restriction is not effective in the prevention of BOP induced pancreatic carcinogenesis in the Syrian hamster.     

Inhibition of sickle beta-chain (betaS)-dependent polymerization by nonhuman alpha-chains. A superinhibitory mouse-horse chimeric alpha-chain

Horse alpha-chain inhibits sickle beta-chain-dependent polymerization; however, its inhibitory potential is not as high as that of mouse alpha-chain. Horse alpha-(1-30) and alpha-(31-141) segments make, respectively, minor and major contributions to the inhibitory potential of horse alpha-chain. The sum of the inhibitory potential of the two segments does not account for the inhibitory potential of the full-length horse alpha-chain. Although the polymerization inhibitory potential of horse alpha-chain is lower than mouse alpha-chain, the inhibitory potential of horse alpha-(31-141) is comparable to that of mouse alpha-(31-141). When mouse alpha-(1-30) is stitched to horse alpha-(31-141), the product is a chimeric alpha-chain with an inhibitory potential greater than mouse alpha-chain. In contrast, the stitching of horse alpha-(1-30) with mouse alpha-(31-141) had no additional inhibitory potential. Molecular modeling studies of HbS containing the mouse-horse chimeric alpha-chain indicate altered side-chain interactions at the alpha1beta1 interface when compared with HbS. In addition, the AB/GH corner perturbations facilitate a different stereochemistry for the interaction of the epsilon-amino group of Lys-16(alpha) with the beta-carboxyl group of Asp-116(alpha), resulting in a decrease in the accessibility of the side chain of Lys-16(alpha) to the solvent. Based on molecular modeling, we speculate that these perturbations by themselves, or in synergy with the altered conformational aspects of the alpha1beta1 interactions, represent the molecular basis of the superinhibitory potential of the mouse-horse chimeric alpha-chains.     

Linking workplace and homeplace: Relations between the nature of adults' work and their parenting behaviors.

A total of 188 employed mothers and fathers and their 5–7 yr-old child participated in a study of the relations between the nature of adults' work and their parenting attitudes and behaviors (a line of research influenced by both socialization and stress perspectives). In general, positive features of work (i.e., complexity of work with people, challenge, and stimulation) tended to be associated with both self-report and observational measures of developmentally sound parenting (e.g., less harsh discipline, more warmth and responsiveness). Interaction effects indicated that gender of parent and child further condition these relations. Analyses partially support the interpretation that conditions of work influence parenting through their effect on mood but also suggest that certain conditions of work may directly socialize behavior in the parental role (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The photopolymerisation of methacrylic acid by colloidal semiconductors

Summary Methacrylic acid was photopolymerised using CdS and composite, CdS/HgS and CdS/TiO₂, colloidal semiconductor particles as initiators. The previously proposed photoinitiation mechanism involving the photogenerated positive hole in the valence band of the CdS colloid was confirmed by the electron scavenging action of TiO₂. The effect of pH on the course of the polymerisation was investigated and is discussed.     

Amplitude-modulation detection in single-stage negative-feedback amplifiers due to interfering out-of-band signals

The electromagnetic-interference effects of out-of-band signals on negative-feedback amplifiers are investigated. It is assumed that the interfering signals picked up at the input of the amplifier are dominant. Due to the nonlinear behavior of the active devices in the amplifier, unwanted dc shifts and amplitude-modulation (AM) detection may occur. Describing these effects is usually done by using the Volterra series. Although providing good results when used for analysis, methods to use the Volterra series for the design process are not yet mature enough. A simple procedure for calculating AM detection is presented. An equivalent source at the input of the amplifier is introduced that accounts for the nonlinear effects. Because the characteristics of this source are computed as a function of design parameters, this procedure facilitates a synthesis approach for designing amplifiers with a lower susceptibility to AM detection. It is shown that the root locus of the amplifier transfer function has a large influence on the amount of AM detection and, therefore, on the electromagnetic compatibility. Measurements made on a single-stage negative-feedback amplifier support the presented procedure.     

Unsteady two-film model for mass transfer

The classical two-film model for mass transfer between two fluid phases can only be used to describe the steady state mass transfer mechanism. Both the penetration and the film-penetration model are also valid solely when only one diffusion boundary layer exists at the interface between the phases. With a view to surmounting the limitations of the above models a new model has been developed for the mass transfer between two fluid phases with two diffusion boundary layers at the interface. The new model is based on the two-film concept and the Danckwerts distribution function, and is also able to simulate the unsteady state mass transfer mechanism between two fluid phases with two different boundary layers.     

Influence of the temperature profile in the interface on the bond strength of polyamide–polyurethane two‐component tensile bars

Two‐component tensile bars with polyamide 6 (PA) and thermoplastic polyurethane (TPU) unmodified and modified with 4,4′‐diphenylmethane diisocyanate (MDI) have been investigated. It is known that the bond strength of PA/TPU tensile bars can increase by about 50% if MDI‐modified polyurethane is applied. The subject of these studies is the analysis of the influence of the mass temperatures on the bond strength. The mass temperatures of the first and second injection process and the delay time between the first and second injection processes determine the temperature gradient in the interface at the moment of the second injection. There is an increase in the bond strength when there is an increasing temperature gradient; therefore, the mass temperature of the first component at the delay time has to be lower than the mass temperature of the second component. The temperature gradient can be used as a measure of the thermal annealing of the first component near the interface during the heat transfer from the second component. It can be concluded that effective thermal annealing causes an increase in the bond strength. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 4297–4305, 2006