Dependence on the motif YIPP for the physical association of Jak2 kinase with the intracellular carboxyl tail of the angiotensin II AT1 receptor

Angiotensin II is the effector molecule of the renin-angiotensin system. Virtually all of its biochemical actions are mediated through a single class of cell-surface receptors called AT1. These receptors contain the structural features of the seven-transmembrane, G-protein-coupled receptor superfamily. Angiotensin II, acting through the AT1 receptor, also stimulates the Jak/STAT pathway by inducing ligand-dependent Jak2 tyrosine phosphorylation and activation. Here, we show that a glutathione S-transferase fusion protein containing the carboxyl-terminal 54 amino acids of the rat AT1A receptor physically binds to Jak2 in an angiotensin II-dependent manner. Deletional analysis, using both in vitro protocols and cell transfection analysis, showed that this association is dependent on the AT1A receptor motif YIPP (amino acids 319-322). The wild-type AT1A receptor can induce Jak2 tyrosine phosphorylation. In contrast, an AT1A receptor lacking the YIPP motif is unable to induce ligand-dependent phosphorylation of Jak2. Competition experiments with synthetic peptides suggest that each of the YIPP amino acids, including tyrosine 319, is important in Jak2 binding to the AT1A receptor. The binding of the AT1A receptor to the intracellular tyrosine kinase Jak2 supports the concept that the seven-transmembrane superfamily of receptors can physically associate with enzymatically active intracellular proteins, creating a signaling complex mechanistically similar to that observed with growth factor and cytokine receptors.     

An automated coding and classification system with supporting database for effective design of manufacturing systems

The philosophy of group technology (GT) is an important concept in the design of flexible manufacturing systems and manufacturing cells. Group technology is a manufacturing philosophy that identifies similar parts and groups them into families. Beside assigning unique codes to these parts, group technology developers intend to take advantage of part similarities during design and manufacturing processes. GT is not the answer to all manufacturing problems, but it is a good management technique with which to standardize efforts and eliminate duplication. Group technology classifies parts by assigning them to different families based on their similarities in: (1) design attributes (physical shape and size), and/or (2) manufacturing attributes (processing sequence). The manufacturing industry today is process focused; departments and sub units are no longer independent but are interdependent. If the product development process is to be optimized, engineering and manufacturing cannot remain independent any more: they must be coordinated. Each sub-system is a critical component within an integrated manufacturing framework. The coding and classification system is the basis of CAPP and the functioning and reliability of CAPP depends on the robustness of the coding system. The proposed coding system is considered superior to the previously proposed coding systems, in that it has the capability to migrate into multiple manufacturing environments. This article presents the design of a coding and classification system and the supporting database for manufacturing processes based on both design and manufacturing attributes of parts. An interface with the spreadsheet will calculate the machine operation costs for various processes. This menu-driven interactive package is implemented using dBASE-IV. Part Family formation is achieved using a KAMCELL package developed in TURBO Pascal.     

Influence of joint detail on the flexural/torsional interaction of thin-walled structures

For a structure formed from two thin-walled open members connected at 90°, torsion applied to one member can result in torsion as well as flexure in the second member, with the magnitude and direction of this torsion as well as flexure in the second member being determined by the type of joint used. Conventional structural analysis would normally assume the presence of only flexure in the second member. The results from a finite element study of structures formed from thin-walled channel sections connected by box, mitre and stiffened mitre joints is presented and an explanation for the behaviour of the different joint types is given. It is shown that for the box joint the warping deformation of the loaded member is the dominant factor in determining the magnitude and direction of the twisting of the second member, whilst this is determined for the stiffened mitre joint primarily by the St Venant rotation deformation of the loaded member. For the unstiffened joint it is shown that the warping and St Venant rotation deformation effects tend to cancel each other out.     

利用钨极氩弧焊技术修复1#汽轮机转子的实践

本文主要介绍了我厂利用钨极氩弧焊将1#发电机组汽轮机转子修复的工艺过程.     

Impulse breakdown voltages of triggered multistage vacuum gap

Several sealed-off triggered vacuum gaps are connected in series to improve hold-off voltage. The characteristics of impulse breakdown voltage of these series-connected gaps are investigated experimentally. The sum hold-off voltage of series-connected gaps decreases to a unit hold-off voltage when the maximum value of voltage division ratio across the gaps increases to unity. Self-breakdown probability of the series-connected gaps is always higher than that of a single gap under the same conditions. Hence, stage efficiency of the multistage gap decreases with increasing number of stages. Its value is 90 percent with 2-stage gap and 75 percent with 5-stage gap, respectively, under the same voltage division ratio and the same gap length (2.0 mm) in each stage. Triggered breakdown voltage of 2- or 3- stage gap is several hundred volts when all gaps are triggered simultaneously at the peak of the main impulse wave and a working voltage range is nearly 100 percent in this case. The working voltage range decreases with number of stages. Its value is 45 percent with 3-stage gap and 15 percent with 5-stage gap, respectively, when one triggered gap is fired for switching.     

Thermoelastic solutions for orthotropic and anisotropic composite laminates

Solutions, within the framework of linear uncoupled thermoelasticity, are presented for certain problems of flexure of composite laminates. Benchmark numerical results, useful for the validation or otherwise of approximate laminate models, are tabulated. Finally, these results are used to examine the accuracy of the classical lamination theory based on Kirchhoff's hypothesis.     

Zooplankton population in the river tigris: Effects of samarra impoundment

The influences of the Samarra impoundment on the ecology of the downstream sector of the river Tigris are investigated. Zooplankton were collected monthly from July 1987 to July 1988. It was found that zooplankton were most abundant during high river discharges in March. During the month of low river discharge, September, the zooplankton population was greatly reduced immediately below the barrage and remained so to the last downstream station. Several factors seem to explain such differences. The high discharge from the impoundment was found to be the dominating factor and may flush the small backwaters in which zooplankton were abundant, thus increasing the population in the river. Zooplankton species vary in their ability to sustain populations in the river, variations which are due mainly to species-specific characteristics.     

深斜井永久式封隔器两种套铣方式风险分析与优化措施

在深斜井大修作业中,为了适应海上修井机作业能力的需要,需要处理井下永久式封隔器。采用磨铣方式处理难度大、周期长、费用高,因此通常采取套铣方式,仅将可钻式永久性封隔器卡瓦及密封套铣掉,再打捞回收或下推至人工井底,直至不妨碍下一步作业。从作业井实际情况出发,针对深斜井永久式封隔器,采用了两种不同动力驱动方式进行套铣处理:一种是地面转盘驱动,井下全部钻具都处于旋转状态,全程摩阻及扭矩大,可以施加较大钻压;另一种为井下马达驱动,即只有马达以下钻具旋转,而马达以上钻具不转,从而降低摩阻,但施加钻压较小,否则容易引起马达失速,所以对马达性能要求较高。通过套铣风险对比分析,分别对两种套铣钻具与参数进行了优化,提出了套铣成功与否的判断依据,为现场应用提供了借鉴。