In vitro comparison of hepatic metabolism of 9cis-11 trans and 10trans-12cis isomers of CLA in the rat

Hepatic metabolism of the two main isomers of CLA (9cis-11 trans, 10trans-12cisC_18∶2) was compared to that of oleic acid (representative of the main plasma FA) in 16 rats by using the in vitro method of incubated liver slices. Liver tissue samples were incubated at 37°C for 17h under an atmosphere of 95% O₂/5%CO₂ in a medium supplemented with 0.75 mM of FA mixture (representative of circulating nonesterified FA) and with 55 μM [1-¹⁴C]9cis-11 trans C_18∶2, [1-¹⁴C]10trans-12cis C_18∶2, or [1-¹⁴C]oleate. The uptake of CLA by hepatocytes was similar for both isomers (9%) and was three times higher (P<0.01) than for oleate (2.6%). The rate of CLA isomer oxidation was two times higher (49 and 40% of incorporated amounts of 9cis-11 trans and 10trans-12 cis, respectively) than that of oleate (P<0.01). Total oxidation of oleate and CLA isomers into [¹⁴CO₂] was low (2 to 7% of total oxidized FA) compared to the partial oxidation (93 to 98%) leading to the production of [¹⁴C] acid-soluble products. CLA isoemrs escaping from catabolism were both highly desaturated (26.7 and 26.8%) into conjugated 18∶3. Oleate and CLA isomers were mainly esterified into neutral lipids (30%). They were slowly secreted as parts of VLDL particles (<0.4% of FA incorporated into cells), the extent of secretion of oleate and of 10trans-12 cis being 2.2-fold higher than that of 9cis-11 trans (P<0.02). In conclusion, this study clearly showed that both CLA isomers were highly catabolized by hepatocytes, reducing their availability for peripheral tissues. Moreover, more than 25% of CLA escaping from catabolism was converted into conjugated 18∶3, the biological properties of which remain to be elucidated.     

基于Java的建筑能耗监控系统

通过MyEclipse10.0和SQL Server 2008开发了一个建筑能耗监控平台,系统采用Java语言的JDBC数据库访问技术,SQL Server 2008数据库语言以及Java语言中的图形化库等,实时更新显示能耗数据,并对数据进行分析和管理,有效地实现了监控的目的。     

护肤品新剂型的研究

介绍了中药护肤品－成团型涂抹膏新产品研究的相关实验，主要包括基质实验，药物选择实验等。     

中长期负荷预测研究与管理一体化应用平台的构建

论文提出了一种构建中长期负荷预测研究与管理一体化应用平台的设想.平台中,建立了经济、能源及电力生产数据的数据库,实现了经济与用电典型样本监测,建立电力与经济预测系统.该平台主要实现中长期负荷预测的相关研究、分析、预测功能,实现对于负荷预测相关工作的全过程管理,共享基础数据资源与预测结论,规范预测流程,统一预测指标体系,能有效提高工作效率与质量.该平台已在国网安徽省电力公司建设实现,并已试运行.     

Effects of conjugated linoleic acid isomers on the hepatic microsomal desaturation activities in vitro

The influence of individual conjugated linoleic acid (CLA) isomers on the Δ6 desaturation of linoleic and α-linolenic acids and on the Δ9 desaturation of stearic acid was investigated in vitro, using rat liver microsomes. The Δ6 desaturation of 18∶2n−6 was decreased from 23 to 38% when the ratio of 9cis,11trans-18∶2 to 18∶2n−6 increased from 0.5 to 2. The compound 10trans,12cis-18∶2 exhibited a similar effect only at the highest concentration. The Δ6 desaturation of α-linolenic acid was slightly affected by the presence of CLA isomers. The sole isomer to induce an inhibitory effect on the Δ9 desaturation of stearic acid was 10trans,12cis-18∶2.     

遗传算法理论及其应用研究进展

首先阐述遗传算法的原理和求解问题的一般过程;然后讨论了近年来从遗传算子、控制参数等方面对遗传算法的改进,并对遗传算法在计算机科学与人工智能、自动控制以及组合优化等领域的应用进行陈述;最后评述了遗传算法未来的研究方向和主要研究内容。     

基于GPS实时数据的在线过滤与补遗研究*

为提高GPS数据的有效性与可靠性,使之为实现实时交通流量预测与交通诱导服务,首先基于GPS历史数据,以变异系数极小化为优化目标进行GPS数据过滤模型的优选,并对实时数据缺损提出两种补遗算法;然后给出基于动态GPS实时数据的在线数据过滤与补遗一体化算法;最后结合2008年杭州市GPS历史数据对模型进行应用。结果表明,基于简单算术平均的滤波模型即为GPS数据过滤的最佳模型;基于时间序列缺失数据的快速补遗算法能够很好地满足实时预报要求,实现快速补遗。     

基于Blowfish和MD5的混合加密方案*

针对Blowfish算法在实际应用中存在的等价密钥、重复初始化等问题,提出Blowfish与MD5算法相结合,将MD5算法的生成值作为密钥的一部分对Blowfish算法进行初始化,使用概率统计方法证明该方案弥补了Blowfish算法的实际应用缺陷。计算结果表明,混合加密方案具有更高的安全性、更快的速度。     

Ad hoc网络中动态阈值岭型函数退避算法*

无线自组织网络(Ad hoc)在MAC层中使用IEEE 802.11 DCF的接入机制来解决节点传输竞争问题,为了解决高负载环境下的网络拥塞状况问题,在退避算法中加入了动态阈值和岭型函数来降低数据传输的冲突概率并利用NS2对改进的退避算法进行网络仿真。仿真结果表明,相对于传统的退避算法,在负载较重的网络中改进后的退避算法大概能够提高网络性能20%左右,优化后网络的时延、吞吐量和公平性均得到明显改善。