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51.
This paper reports preliminary computational fluid dynamics (CFD) simulations of backdraft observed in an experimental rig at Lund University. The analysis was performed with the CFX software using the Detached Eddy Simulation (DES) turbulence model, a hybrid of Large Eddy Simulation (LES) and RANS, in combination with the EDM combustion model. The DES model uses a RANS formulation in wall proximity to avoid computationally expensive grid resolution that is necessary for realistic LES predictions in wall layers. 相似文献
52.
Danijel Rebolj Andrej Tibaut Nenad u-Babi
Ale Magdi
Peter Podbreznik 《Automation in Construction》2008,17(6):719-728
Product modeling is not a new technology any more, but its use in construction industry is still very rare, even more in the area of road design, construction and maintenance. The paper presents the evolution of a road product model, PMC, from its early concepts in 1993 to the latest implementations and applications. It explains the basic structure of the model and compares the PMC with other existing road models. Some software modules based on the latest version of the PMC road product model are presented. Further the paper presents experiences and findings of a case study, where the model and the relevant software has been tested. The paper concludes with a discussion on effectiveness of the PMC and suggests next steps for a broader acceptance of the presented technology. 相似文献
53.
Schwudke D Hannich JT Surendranath V Grimard V Moehring T Burton L Kurzchalia T Shevchenko A 《Analytical chemistry》2007,79(11):4083-4093
Direct profiling of total lipid extracts on a hybrid LTQ Orbitrap mass spectrometer by high-resolution survey spectra clusters species of 11 major lipid classes into 7 groups, which are distinguished by their sum compositions and could be identified by accurately determined masses. Rapid acquisition of survey spectra was employed as a "top-down" screening tool that, together with the computational method of principal component analysis, revealed pronounced perturbations in the abundance of lipid precursors within the entire series of experiments. Altered lipid precursors were subsequently identified either by accurately determined masses or by in-depth MS/MS characterization that was performed on the same instrument. Hence, the sensitivity, throughput and robustness of lipidomics screens were improved without compromising the accuracy and specificity of molecular species identification. The top-down lipidomics strategy lends itself for high-throughput screens complementing ongoing functional genomics efforts. 相似文献
54.
Rühl R Hänel A Garcia AL Dahten A Herz U Schweigert FJ Worm M 《Molecular nutrition & food research》2007,51(9):1173-1181
Vitamin A (VA) and its derivatives, the retinoids, are important factors for the development of the immune system. It has been shown in adult animals that proliferation of lymphocyte populations and antibody secretion are retinoid dependent, while little is known about the effects of retinoids during postnatal development. The aim of this study was to investigate the role of VA on allergic sensitisation during lactation and after weaning using an in vivo system for postnatal allergic sensitisation in mice. Different VA diets (basal/VA elimination/VA (as retinyl palmitate) supplemented) were fed to the dams throughout lactation and directly to the pups after weaning. Allergic sensitisation was induced with a single peritoneal ovalbumin (OVA) injection at day 28 after weaning. The phenotype of lymphocytes was analysed by flow cytometry and functional data were obtained by analysis of (IL-4/IFN-gamma) cytokine production and antibody production (OVA-specific IgG1 and IgE) in the offspring. VA/retinyl palmitate supplementation during lactation and after weaning decreased CD3+, CD4+, CD8+ and B220+ populations in splenic lymphocytes but also significantly enhanced IL-4 production and OVA-specific IgE after sensitisation. In contrast, mice fed VA-elimination diet displayed no significant alteration of lymphocyte numbers and a slightly increased IL-4 production. Our results showed that a single allergen injection during postnatal development induces allergic sensitisation whose degree is modified by the VA content of the maternal diet during lactation and the diet of the pups after weaning, indicating an important role of VA on the severity of the allergic sensitisation. 相似文献
55.
Yen T. H. Lam Manuel G. Ricardo Robert Rennert Andrej Frolov Andrea Porzel Wolfgang Brandt Pauline Stark Bernhard Westermann Norbert Arnold 《International journal of molecular sciences》2021,22(23)
Fungal species of genus Sepedonium are rich sources of diverse secondary metabolites (e.g., alkaloids, peptaibols), which exhibit variable biological activities. Herein, two new peptaibols, named ampullosporin F (1) and ampullosporin G (2), together with five known compounds, ampullosporin A (3), peptaibolin (4), chrysosporide (5), c(Trp-Ser) (6) and c(Trp-Ala) (7), have been isolated from the culture of Sepedonium ampullosporum Damon strain KSH534. The structures of 1 and 2 were elucidated based on ESI-HRMSn experiments and intense 1D and 2D NMR analyses. The sequence of ampullosporin F (1) was determined to be Ac-Trp1-Ala2-Aib3-Aib4-Leu5-Aib6-Gln7-Aib8-Aib9-Aib10-GluOMe11-Leu12-Aib13-Gln14-Leuol15, while ampullosporin G (2) differs from 1 by exchanging the position of Gln7 with GluOMe11. Furthermore, the total synthesis of 1 and 2 was carried out on solid-phase to confirm the absolute configuration of all chiral amino acids as L. In addition, ampullosporin F (1) and G (2) showed significant antifungal activity against B. cinerea and P. infestans, but were inactive against S. tritici. Cell viability assays using human prostate (PC-3) and colorectal (HT-29) cancer cells confirmed potent anticancer activities of 1 and 2. Furthermore, a molecular docking study was performed in silico as an attempt to explain the structure-activity correlation of the characteristic ampullosporins (1–3). 相似文献
56.
In this study, the gel strength and visible microstructure of fat containing β-lactoglobulin-κ-carrageenan gels were investigated using puncture testing and confocal scanning laser microscopy, respectively. The gel strength was closely linked to the visible microstructure of the whey protein network as stained with Rhodamine B. Covalent labelling of κ-carrageenan with FITC prior to gel formation enabled localisation of the hydrocolloid phase, but caused a significant drop in the gel strength. This effect coincided with the observed reduction of the κ-carrageenan intrinsic viscosity, which was found to be a result of the labelling process. The use of a novel dye, V03-01136, for the staining of fat allowed for the specific and concurrent visualisation of the protein, hydrocolloid and fat phases under the conditions applied in the study. 相似文献
57.
A Grubisic E Ringe CM Cobley Y Xia LD Marks RP Van Duyne DJ Nesbitt 《Nano letters》2012,12(9):4823-4829
Electron emission from single, supported Ag nanocubes excited with ultrafast laser pulses (λ = 800 nm) is studied via spatial and polarization correlated (i) dark field scattering microscopy (DFM), (ii) scanning photoionization microscopy (SPIM), and (iii) high-resolution transmission electron microscopy (HRTEM). Laser-induced electron emission is found to peak for laser polarization aligned with cube diagonals, suggesting the critical influence of plasmonic near-field enhancement of the incident electric field on the overall electron yield. For laser pulses with photon energy below the metal work function, coherent multiphoton photoelectron emission (MPPE) is identified as the most probable mechanism responsible for electron emission from Ag nanocubes and likely metal nanoparticles/surfaces in general. 相似文献
58.
Arvind Prasad Peter J. Uggowitzer Zhiming Shi Andrej Atrens 《Advanced Engineering Materials》2012,14(7):477-490
The efficacy of Zr in removing Fe from molten Mg‐X binary alloys was studied experimentally and using calculated phase diagrams. Zr is effective in reducing the Fe content from the Mg melt for Mg‐X binary alloys, X = Y, Si, Sn, Ca, Sr, Ce, Gd, Nd, Li, La, Mn, and Zn. Purification occurs by the precipitation from the melt of Fe rich precipitates, and the settling of the precipitates to the bottom of the melt. Any desired Fe content down to 1 ppm can be achieved by appropriate melt treatment. 相似文献
59.
60.
Luka Snoj Andrej Trkov Igor Lengar Sergey Popovichev Sean Conroy Brian Syme 《Fusion Engineering and Design》2012,87(11):1846-1852
After the coated CFC wall to ITER-Like Wall (Beryllium/Tungsten/Carbon) transition in 2010–11, confirmation of the neutron yield calibration will be ensured by direct measurements using a calibrated 252Cf neutron source deployed by the in-vessel remote handling boom and Mascot manipulator inside the JET vacuum vessel.The paper describes preliminary calculations and the results of numerical study of the effect of source holder on neutron detector response. The source baton was designed in such a way, that it does not significantly affect the neutron spectrum, angular neutron flux distribution or activation detector response. All effects are approximately equal to or less than 1%. The largest disturbance to the neutron flux angular distribution and to the neutron spectrum arises from the source capsule. Hence one should obtain as much information as possible about the capsule and the 252Cf source material in order to avoid additional systematic errors. 相似文献