Electroconvulsive shock does not induce c-fos and junB, but TIS1 and TIS8/zif-268, in neonatal rat hippocampus

Insular neurons responsive to baroreceptor challenge have been identified in the rat, but not previously in primates. Characterization of baroreceptor-related neurons was performed in 15 anesthetized monkeys (Macaca fascicularis) using extracellular single-unit recording techniques. 131 units were investigated within the insula and surrounding regions. Based on their responses to phenylephrine hydrochloride (PE) and sodium nitroprusside (SNP), three types of units were distinguished: 35/131 (27%) sympathoexcitatory (SE), 12/131 (9%) sympathoinhibitory (SI) and 84 (64%) null units. More baroreceptive units were found within the insula (38/73, 52%) than in surrounding areas (9/58, 16%) (p < 0.001). Lateralization was indicated with more baroreceptive units being encountered within the right insula (28/44, 64%) than the left (10/29, 34%) (p = 0.02). The majority of the responsive units were located within the dysgranular and granular insula in layers II, III and V/VI. These data suggest that cardiovascular representation may occur in the primate insula as has been shown in other species.     

Technique for suppression of pattern dependence in a semiconductor-optical-amplifier wavelength converter

Wavelength converters that employ cross-gain modulation (XGM) in semiconductor optical amplifiers (SOAs) are limited in bit rate by the amplifier's pattern dependent response. We substantially suppress this pattern dependence by converting the phase modulation at the data transitions to amplitude modulation using a fiber grating filter. We use this technique to obtain error-free wavelength conversion of 10-Gb/s nonreturn-to-zero (NRZ) data using an SOA that showed an error floor at 10/sup -5/ bit-error rate (BER) when used alone.     

Identification of an NAD+ binding site of brain glutamate dehydrogenase isoproteins by photoaffinity labeling

Photoaffinity labeling with [32P]nicotinamide 2-azidoadenosine dinucleotide (2N3NAD+) was used to identify the NAD+ binding site within two types of glutamate dehydrogenase isoproteins (GDH I and GDH II) isolated from bovine brain. In the absence of photolysis, 2N3NAD+ is a substrate for the GDH isoproteins. When the enzymes were covalently modified by photolysis in the presence of saturating amounts of photoprobe, about 50% inhibition of the GDH activities was observed. Photoinsertion of probe was increased by GTP or glutarate and decreased by NAD+ or ADP. With the combination of immobilized boronate affinity chromatography and reversed-phase HPLC, photolabel-containing peptides generated with trypsin were isolated. This identified a portion of the adenine ring binding domain of GDH isoproteins as the region containing the sequence, CIAVGXSDGSIWNPDGIDPK for both GDH isoproteins, corresponding to Cys270 through Lys289 of the amino acid sequence of well known bovine liver GDH. The X indicates a position for which no phenylthiohydantoin-derivative could be assigned. The missing residue, however, can be designated as a photolabeled glutamate since the sequences including the glutamate residue in question have a complete identity with those of the other GDH species known. Photolabeling of these peptides was prevented by the presence of NAD+ during photolysis. These results demonstrate selectivity of the photoprobe for the NAD+ binding site and suggest that the peptide identified using the photoprobe is located in the NAD+ binding domain of the brain GDH isoproteins. Both amino acid sequencing and compositional analysis identified Glu275 as the site of photoinsertion.     

Extension of the boundary element method to systems with conductorsand piece-wise constant dielectrics

Accurate modeling of electric fields is necessary to study the dynamics of microelectromechanical systems (MEMS). Typical microelectromechanical systems use high dielectric-constant materials, however, which significantly affect the electric field. Many numerical packages use the finite element method (FEM) to deal with systems with dielectrics. The FEM is not, however, very efficient in modeling microelectromechanical systems. There exist numerical packages that use the boundary element method (BEM), which is more desirable due to its low computational cost. But the BEM as it exists cannot model the effects of the dielectrics. This paper extends the BEM to systems with piece-wise constant dielectrics as well as conductors     

An effective heuristic method for generalized job shop scheduling with due dates

This paper is concerned with the generalized job shop scheduling problem with due dates wherein the objective is to minimize total job tardiness. An efficient heuristic algorithm called the revised exchange heuristic algorithm (REHA) is presented for solving this problem. It has been shown that the algorithm can be completed in polynomial time. Results, generated over a range of shop sizes with different due date tightness levels, indicate that the proposed technique is capable of yielding notable reductions in total tardiness (over initial schedules) for practical size problems. This suggests that this approach is an efficient scheduling option for this class of complex optimization problems.     

Thermodynamic criterion of separated eutectic phenomena

About 26 kinds of simple and complex binary eutectics were frozen directionally under the condition of electromagnetic stirring. It was found that anomalous eutectics including most metals/non-metals and some metal/intermetallic eutectics, can be separated on macroscopic scale by flow caused by electromagnetic stirring. Moreover, the eutectics in which one of their component phases possess an entropy of solution over 23 J mol-1K-1 showed notable separation. In addition, process factors also affect the formation of a separated eutectic. © 1998 Chapman & Hall     

Feldkamp and circle-and-line cone-beam reconstruction for 3D micro-CT of vascular networks

Detailed morphometric knowledge of the microvascular network is needed for studies relating structure to haemodynamic function in organs like the lung. Clinical volumetric CT is limited to millimetre-order spatial resolution. Since evidence suggests that small arterioles (50 to 300 micrometres) dominate pulmonary haemodynamics, we built a micro-CT scanner, capable of imaging excised lungs in 3D with 100 microm resolution, for basic physiology research. The scanner incorporates a micro-focal (3 microm) x-ray source, an xyz theta stage and a CCD-coupled image intensifier detector. We imaged phantoms and contrast-enhanced rat lungs, reconstructing the data with either the Feldkamp or the circle-and-line cone-beam reconstruction algorithm. We present reconstructions using 180 views over 360 degrees for the circular trajectory, augmented with views from a linear scan for the circle-and-line algorithm. Especially for platelike features perpendicular to the rotation axis and remote from the midplane, the circle-and-line algorithm produces superior reconstructions compared with Feldkamp's algorithm. We conclude that the use of nonplanar source trajectories to perform micro-CT on contrast-enhanced, excised lungs can provide data useful for morphometric analysis of vascular trees, currently down to the 130 microm level.     

Modeling, analysis, and design of the quasi-charge control

A current-mode control technique, quasi-charge control (QCC), is studied. A small-signal model is developed for QCC. The model is derived for converters operating in the continuous conduction mode. The model is accurate up to half of the switching frequency. The proposed small-signal model is used to assess the dynamics of pulse-width-modulated (PWM) power converters with QCC, and to compare their performance to the current-injected control (CIC) and charge control (CC) schemes. As in CC, the QCC converges to CIC at light loads close to the boundary between continuous and discontinuous conduction modes. The dynamics of power converters with QCC or CC are more sensitive to load change and less sensitive to line change than those of power converters with CIC, especially the current loop dynamics. However, unlike in the case of CC, the dependency on the load/line can be controlled when the QCC is designed. Control design guidelines for DC-DC power converters and power factor correction rectifiers are presented, and experimental measurements are performed to verify the proposed model