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排序方式: 共有979条查询结果,搜索用时 15 毫秒
71.
Nicolas Vergauwe Daan Witters Yegermal T. Atalay Bert Verbruggen Steven Vermeir Frederik Ceyssens Robert Puers Jeroen Lammertyn 《Microfluidics and nanofluidics》2011,11(1):25-34
Although several applications of electrowetting on dielectric digital lab-on-a-chips are reported in literature, there is
still a lack of knowledge about the influence of operational and design parameters on the performance of an analytical assay.
This paper investigates how droplet size variability, introduced by droplet dispensing and splitting, influences the assay
performance with respect to repeatability and accuracy and presents a novel method to reduce this variability. Both a theoretical
and experimental approach were followed. Monte Carlo simulations were applied to study the cumulative effect of the variability
caused by different droplet manipulations on the final assay performance. It is shown that a highly controllable droplet generation
and manipulation is achieved with respect to droplet size variability through an accurate control of actuation voltage, activation
time, relaxation time, and electrode size. As a case study, it is illustrated that through optimization of these parameters
a complete on-chip calibration curve is obtained for a d-glucose assay with an average CV-value of 2%. These new insights aim to bring the digital lab-on-a-chip technology closer
to researchers in the field of diagnostics offering them a valuable and accessible alternative to standard analysis platforms. 相似文献
72.
Graphene sheets can be regarded as base structure of many carbon nanostructures, and atomic arrangements and variations in the atomic structure have a drastic impact on their unique properties. Using a single/double layer graphene model structure, we present a strategy to “see” single carbon atoms in 3-D. In high-resolution transmission electron microscopy, exit-wave images are essential to obtain and to understand 3-D atomic structure. Using electrons at 80 kV not only minimizes the knock-on damage, but also improves the detection sensitivity due to the higher scattering power of carbon at lower acceleration voltage of the electron. Using experiments and image simulations, positions of individual carbon atoms in a single/double layer structure in graphene have been identified. 相似文献
73.
Accurate convective heat transfer predictions are required in building engineering and environmental studies on urban heat islands, building energy performance, building-envelope durability or conservation and (natural) ventilation of buildings. When applying computational fluid dynamics (CFD) for these computationally-expensive studies at high-Reynolds numbers, wall functions are mostly used to model the boundary-layer region. In this study, an adjustment to the standard temperature wall function is proposed for forced convective heat transfer at surfaces of typical wall-mounted bluff bodies in turbulent boundary layers, such as the atmospheric boundary layer, at moderate to high Reynolds numbers. The methodology to determine this customised temperature wall function (CWF) from validated numerical data of CFD simulations using low-Reynolds number modelling (LRNM) is explained, where a logarithmic- law behaviour is found. The performance of this CWF is evaluated for several bluff-body configurations. Standard wall functions (SWFs) yield deviations of about 40% for the convective heat transfer coefficient, compared to LRNM. With the CWF however, these deviations are reduced to about 10% or lower. The CWF therefore combines increased (wall-function) accuracy for convective heat transfer predictions with the typical advantage of wall functions compared to LRNM, being a lower grid resolution in the near-wall region, which increases computational economy and facilitates grid generation. Furthermore, this CWF can be easily implemented in existing CFD codes, and is implemented in the commercial CFD code Fluent in this study. 相似文献
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An objective of the study was to identify leaf morphophysiological characteristics indicative of high aboveground woody biomass production of Salix clones growing in the vegetation filter of a wastewater purification system. It was hypothesized, that in these extra irrigated stands, the characteristics of photosynthesis are stronger determinants of the production than the characteristics of leaf water relations. The biomass production of the clones was correlated with numerous characteristics of leaf water relations and photosynthesis. Significant correlation was found between the biomass production and the following characteristics of top foliage leaves: daily decrease in water potential, stomatal conductance, stomatal sensitivity to increase in light intensity, and hydraulic conductance (positive); daily minimum and maximum water potentials, instantaneous water use efficiency, and stomatal sensitivity to decrease in leaf water potential (negative); maximum rate of photosynthetic electron transport, chlorophyll content, and the partitioning coefficient for leaf nitrogen into bioenergetics associated with electron transport (positive). In the most productive clones, the vertical gradient (through foliage) of values of almost all the characteristics was the smallest. The biomass correlated more strongly with the characteristics of photosynthesis light stage than with the characteristics of water relations. We conclude that the characteristics of photosynthesis light stage – high maximum rate of photosynthetic electron transport, high chlorophyll content, and also flat vertical gradient (through foliage) in values of the characteristics could be the strongest indicators of high biomass production of Salix clones growing in vegetation filter. 相似文献
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79.
Bert Fraser-Reid G. Anilkumar Latha G. Nair Lars Olsson Mercedes Garcia Martin Jacquitta K. Daniels 《Israel journal of chemistry》2000,40(3-4):255-262
The C1 and C2 stereocenters of α-glucosaminides can be prepared by establishing the stereocenters in either order. For the former, a C2-azido glucosyl donor is prepared first, and the restraining effect of a 4,6-O-benzylidene ring is used to induce α-coupling. For the latter, the C1 linkage is prepared first by use of an n-pentenyl-manno-1,2-orthoester donor which ensures (a) clean α-coupling and (b) a convenient C2-ester. The C2-ester is replaced with a triflate leaving group, and nucleophilic displacement is effected by use of a hypervalent silicon azide. 相似文献
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