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11.
Brett J. Kagan Charlotte M. Ermine Stefano Frausin Clare L. Parish Jess Nithianantharajah Lachlan H. Thompson 《International journal of molecular sciences》2021,22(9)
Neonatal arterial ischemic stroke is one of the more severe birth complications. The injury can result in extensive neurological damage and is robustly associated with later diagnoses of cerebral palsy (CP). An important part of efforts to develop new therapies include the on-going refinement and understanding of animal models that capture relevant clinical features of neonatal brain injury leading to CP. The potent vasoconstrictor peptide, Endothelin-1 (ET-1), has previously been utilised in animal models to reduce local blood flow to levels that mimic ischemic stroke. Our previous work in this area has shown that it is an effective and technically simple approach for modelling ischemic injury at very early neonatal ages, resulting in stable deficits in motor function. Here, we aimed to extend this model to also examine the impact on cognitive function. We show that focal delivery of ET-1 to the cortex of Sprague Dawley rats on postnatal day 0 (P0) resulted in impaired learning in a touchscreen-based test of visual discrimination and correlated with important clinical features of CP including damage to large white matter structures. 相似文献
12.
Kazadi N. Mutoji Mingxia Sun Garrett Elliott Isabel Y. Moreno Clare Hughes Tarsis F. Gesteira Vivien J. Coulson-Thomas 《International journal of molecular sciences》2021,22(11)
Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs). PGs are essential for correct collagen fibrillogenesis and maintaining corneal homeostasis. We investigated the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs after a chemical injury. The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas. The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed. We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS. Expression of the CSPGs biglycan and versican was increased after AB, while decorin expression was decreased. We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression. No significant changes in HS composition were noted after AB. Taken together, our study reveals significant changes in the composition of the extracellular matrix following a corneal chemical injury. 相似文献
13.
Tjokorde W. Samadhi Linda E. Jones Alexis G. Clare 《Journal of the American Ceramic Society》2003,86(12):2044-2049
Carbon reacts with Na2 SO4 in glass batches, influencing SO x emissions. To study the role of carbon active surface area (ASA) in the decomposition phenomena, Na2 SO4 is reacted with three carbons with a wide ASA range. The decomposition behavior was evaluated by thermogravimetric analysis (TGA) and evolved gas analysis via mass spectrometry and Fourier transform infrared spectrometry. Higher carbon ASA yields lower decomposition temperatures. SO x emissions are realized at temperatures as low as 773 K. Isothermal Na2 SO4 –C decomposition initiates via the nucleation of Na2 S. The rate constants for the nucleation regime are normalized against ASA, yielding an activation energy of 275 kJ/mol for nucleation. 相似文献
14.
Office workers perform tasks using different information and communication technologies (ICT) involving various postures. Adequate variation in postures and muscle activity is generally believed to protect against musculoskeletal complaints, but insufficient information exists regarding the effect on postural variation of using different ICT. Thus, this study among office workers aimed to determine and compare postures and postural variation associated with using distinct types of ICT. Upper arm, head and trunk postures of 24 office workers were measured with the Physiometer® over a whole day in their natural work and away-from-work environments. Postural variation was quantified using two indices: APDF(90-10) and EVA(sd). Various ICT had different postural means and variation. Paper-based tasks had more non-neutral, yet also more variable postures. Electronics-based tasks had more neutral postures, with less postural variability. Tasks simultaneously using paper- and electronics-based ICT had least neutral and least variable postures. Tasks without ICT usually had the most posture variability. Interspersing tasks involving different ICT could increase overall exposure variation among office workers and may thus contribute to musculoskeletal risk reduction. 相似文献
15.
O'Reilly-Wapstra JM Potts BM McArthur C Davies NW Tilyard P 《Journal of chemical ecology》2005,31(3):519-537
Hybridization in plants provides an opportunity to investigate the patterns of inheritance of hybrid resistance to herbivores, and of the plant mechanisms conferring this resistance such as plant secondary metabolites. We investigated how inter-race differences in resistance of Eucalyptus globulus to a generalist mammalian herbivore, Trichosurus vulpecula, are inherited in their F1 hybrids. We assessed browsing damage of three-year-old trees in a common environment field trial on four hybrid types of known progeny. The progency were artificial intra-race crosses and reciprocal inter-race F1 hybrids of two geographically distinct populations (races) of E. globulus; north-eastern Tasmania and south-eastern Tasmania. Populations of trees from north-eastern Tasmania are relatively susceptible to browsing by T. vulpecula, while populations from south-eastern Tasmania are more resistant. We assessed the preferences of these trees in a series of paired feeding trials with captive animals to test the field trial results and also investigated the patterns of inheritance of plant secondary metabolites. Our results demonstrated that the phenotypic expression of resistance of the inter-race F1 hybrids supported the additive pattern of inheritance, as these hybrids were intermediate in resistance compared to the pure parental hybrids. The expression of plant secondary metabolites in the F1 hybrids varied among groups of individual compounds. The most common pattern supported was dominance towards one of the parental types. Together, condensed tannins and essential oils appeared to explain the observed patterns of resistance among the four hybrid types. While both chemical groups were inherited in a dominant manner in the inter-race F1 hybrids, the direction of dominance was opposite. Their combined concentration, however, was inherited in an additive manner, consistent with the phenotypic differences in browsing. 相似文献
16.
This article focuses on preteen British children's use of text messaging, particularly the abbreviations and characteristic language used within text messages. We look not only at the language style used by the children, but at the ways in which it relates to their traditional literacy skills, as measured through standardized tests and assessments. We have found repeated positive relationships between use of text register language and traditional literacy skills, and we give here a preview of our latest, longitudinal work, which allows those relationships to be studied directionally, enabling us to draw causal conclusions. 相似文献
17.
The paper discusses a series of driving trials that were conducted to investigate postural stability of disabled drivers and to assess the effectiveness of a representative sample of support aids. Twenty-three disabled drivers with varying levels of physical disability and seven non disabled drivers participated in the study. The test car was equipped with transducers to measure vehicle velocity and acceleration (longitudinal and lateral), steering wheel movement and torque, and the bracing forces exerted by the driver on the steering wheel. Video cameras were installed to record postural support strategies and displacement of the driver and to record deviation of the car from the specified path. Subjective data regarding driver attitudes and acceptance were also collected through the administration of questionnaires. Findings from the study showed that support aids significantly improved driving performance and reduced physical exertion to maintain an upright driving position for disabled drivers. However, ergonomics design aspects regarding the ease of use and acceptance of the support aids by the end users were identified as obstacles to their sustained use in everyday driving. 相似文献
18.
Philippe Dubuisson Robert Frouin Lucile Duforêt Kenneth Voss 《Remote sensing of environment》2009,113(9):1899-1911
A methodology is proposed to infer the altitude of aerosol plumes over the ocean from reflectance ratio measurements in the O2 absorption A-band (759 to 770 nm). The reflectance ratio is defined as the ratio of the reflectance in a first spectral band, strongly attenuated by O2 absorption, and the reflectance in a second spectral band, minimally attenuated. For a given surface reflectance, simple relations are established between the reflectance ratio and the altitude of an aerosol layer, as a function of atmospheric conditions and the geometry of observation. The expected accuracy for various aerosol loadings and models is first quantified using an accurate, high spectral resolution, radiative transfer model that fully accounts for interactions between scattering and absorption. The method is developed for POLDER and MERIS, satellite sensors with adequate spectral characteristics. The simulations show that the method is only accurate over dark surfaces when aerosol optical thickness at 765 nm is relatively large (> 0.3). In this case, the expected accuracy is on the order of ± 0.5 km or ± 0.2 km for POLDER or MERIS respectively. More accurate estimates are obtained with MERIS, since in this case the spectral reflectance ratio is more sensitive to aerosol altitude. However, a precise spectral calibration is needed for MERIS. The methodology is applied to MERIS and POLDER imagery acquired over marine surfaces. The estimated aerosol altitude is compared with in situ lidar profiles of backscattering coefficient measured during the AOPEX-2004 experiment for MERIS, or obtained with the space-borne lidar CALIOP for POLDER. The retrieved altitudes agree with lidar measurements in a manner consistent with theory. These comparisons demonstrate the potential of the differential absorption methodology for obtaining information on aerosol altitude over dark surfaces. 相似文献
19.
20.
The new composite material Textile Reinforced Concrete (TRC) is a promising development which may open up entirely new fields for the application of the construction material concrete. The possible more filigree structures with high quality surfaces make TRC an attractive choice for the architect and give the engineer more freedom in design. However, the use of TRC requires design rules which are currently being developed at RWTH Aachen University, Germany. In this article, recent experimental results as well as modeling techniques are described. 相似文献