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71.
Antonio Qualtieri Dr. Elena Urso Maria Le Pera Sabrina Bossio Francesca Bernaudo Tiziana Ferraro Lucia Crescibene Umberto Aguglia Aldo Quattrone 《Proteomics. Clinical applications》2009,3(5):574-583
MALDI‐TOF protein profiling analysis permits the detection of peptides and small proteins in complex protein mixtures with great accuracy. We applied this analysis to cerebrospinal fluid (CSF) from 15 patients affected by Creutzfeldt‐Jakob disease (CJD). We compared the levels of the normalized ion signals of 11 sporadic and 4 genetic CJD forms with those from ten healthy control subjects and eight non‐CJD relapsing‐remitting multiple sclerosis patients. In so doing, we detected 61 differentially expressed ion signals in CJD samples compared to controls. Among the 61 signals, 3 signals had significantly increased levels with high statistical significance (p <0.0001) and were located at 3238.3 m/z, 4963.7 m/z, and 8565.3 m/z. We characterized the 5.0 and 8.6 kDa proteins as thymosin β4 N‐acetylated and free ubiquitin, respectively, while the 3.2‐kDa peptide remained uncharacterized. Although elevated ubiquitin levels have previously been described in CJD, we have demonstrated for the first time the involvement of thymosin β4 in a neurodegenerative disease. To support the validity of thymosin β4 levels obtained by MALDI‐TOF analysis, an independent enzyme immunoassay analysis was performed. Moreover, a validation cohort consisting of CSF from three CJD patients, five healthy subjects, and six non‐CJD relapsing‐remitting multiple sclerosis patients was analyzed in a similar way, yielding superimposable results. We propose that thymosin β4 is a potential new candidate marker for the ante mortem diagnosis of CJD disease. 相似文献
72.
Elena Molina Mercedes Ramos Pedro J. Martin-álvarez 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1995,201(4):331-335
Stepwise multiple linear regression (SMLR) and principal components regression (PCR) have been used to predict the percentages of cows', goats' and ewes' milk in Iberico cheese, using the results obtained by electrophoretic analysis (PAGE and IEF) of whey proteins, using standard cheeses. Similar predictions of the percentages of milks from the three species were obtained when either SMLR or PCR were applied to the electrophoretic data, i.e. the optical intensity of the electrophoretic bands (PAGE or IEF) of the whey proteins. The root mean square error of prediction in cross-validation (RMSEPCV) was lower than 4% in all cases. 相似文献
73.
Mendoza Abigail María Elena Ramírez Yu Wen 《International Journal of Control, Automation and Systems》2023,21(2):658-670
International Journal of Control, Automation and Systems - This article presents a fuzzy adaptive control law (FACL) designed for tracking the trajectory of a low-scale unmanned aerial vehicle... 相似文献
74.
75.
Elena Lopez Adrien Leygue Emmanuelle Abisset-Chavanne Sebastien Comas-Cardona Christophe Aufrere Christophe Binetruy Francisco Chinesta 《International Journal of Material Forming》2017,10(3):317-328
The fundamental macroscopic material property needed to quantify the flow in a fibrous medium viewed as a porous medium is the permeability. Composite processing models require the permeability as input data to predict flow patterns and pressure fields. As permeability reflects both the magnitude and anisotropy of the fluid/fiber resistance, efficient numerical techniques are needed to solve linear and nonlinear homogenization problems online during the flow simulation. In a previous work the expressions of macroscopic permeability were derived in a double-scale porosity medium for both Newtonian and rheo-thinning resins. In the linear case only a microscopic calculation on a representative volume is required, implying as many microscopic calculations as representative microscopic volumes exist in the whole fibrous structure. In the non-linear case, and even when the porous microstructure can be described by a unique representative volume, microscopic calculation must be carried out many times because the microscale resin viscosity depends on the macroscopic velocity, which in turn depends on the permeability that results from a microscopic calculation. Thus, a nonlinear multi-scale problem results. In this paper an original and efficient offline-online procedure is proposed for the efficient solution of nonlinear flow problems in porous media. 相似文献
76.
E. Paz J. Abenojar Y. Ballesteros F. Forriol N. Dunne J. C. del Real 《Journal of materials science. Materials in medicine》2016,27(4):72
The basic formulation of an acrylic bone cement has been modified by the addition of a block copolymer, Nanostrength® (NS), in order to augment the mechanical properties and particularly the fracture toughness of the bone cement. Two grades of NS at different levels of loading, between 1 and 10 wt.%, have been used. Mechanical tests were conducted to study the behaviour of the modified cements; specific tests measured the bend, compression and fracture toughness properties. The failure mode of the fracture test specimens was analysed using scanning electron microscopy (SEM). The effect of NS addition on the thermal properties was also determined, and the polymerisation reaction using differential scanning calorimetry. It was observed that the addition of NS produced an improvement in the fracture toughness and ductility of the cement, which could have a positive contribution by reducing the premature fracture of the cement mantle. The residual monomer content was reduced when the NS was added. However this also produced an increase in the maximum temperature and the heat delivered during the polymerisation of the cement. 相似文献
77.
Mónica Giménez‐Marqués Elena Bellido Thomas Berthelot Teresa Simón‐Yarza Tania Hidalgo Rosana Simón‐Vázquez África González‐Fernández José Avila Maria Carmen Asensio Ruxandra Gref Patrick Couvreur Christian Serre Patricia Horcajada 《Small (Weinheim an der Bergstrasse, Germany)》2018,14(40)
Controlling the outer surface of nanometric metal–organic frameworks (nanoMOFs) and further understanding the in vivo effect of the coated material are crucial for the convenient biomedical applications of MOFs. However, in most studies, the surface modification protocol is often associated with significant toxicity and/or lack of selectivity. As an alternative, how the highly selective and general grafting GraftFast method leads, through a green and simple process, to the successful attachment of multifunctional biopolymers (polyethylene glycol (PEG) and hyaluronic acid) on the external surface of nanoMOFs is reported. In particular, effectively PEGylated iron trimesate MIL‐100(Fe) nanoparticles (NPs) exhibit suitable grafting stability and superior chemical and colloidal stability in different biofluids, while conserving full porosity and allowing the adsorption of bioactive molecules (cosmetic and antitumor agents). Furthermore, the nature of the MOF–PEG interaction is deeply investigated using high‐resolution soft X‐ray spectroscopy. Finally, a cell penetration study using the radio‐labeled antitumor agent gemcitabine monophosphate (3H‐GMP)‐loaded MIL‐100(Fe)@PEG NPs shows reduced macrophage phagocytosis, confirming a significant in vitro PEG furtiveness. 相似文献
78.
Elena Baralis Stefano Ceri Piero Fraternali Stefano Paraboschi 《Journal of Intelligent Information Systems》1996,7(2):129-149
The lack of tools for rule generation, analysis, and run-time monitoring appears one of the main obstacles to the widespreading of active database applications. This paper describes a complete tool environment for assisting the design of active rules applications; the tools were developed at Politecnico di Milano in the context of the IDEA Project, a 4-years Esprit project sponsored by the European Commission which was launched in June 1992. We describe tools for active rule generation, analysis, debugging, and browsing; rules are defined in Chimera, a conceptual design model and language for the specification of active rules applications. We also introduce a tool for mapping from Chimera into Oracle, a relational product supporting triggers.Most of the tools described in this paper are fully implemented and currently in operation (beta-testing) within the companies participating to the IDEA Project, with the exception of two of them (called Argonaut-V and Pandora), which will be completed by the end of 1996.Research presented in this paper is supported by Esprit project P6333 IDEA, and by ENEL contract VDS 1/94: Integrity Constraint Management 相似文献
79.
David Schleicher Luis M. Bergasa Manuel Ocaña Rafael Barea Elena López 《Robotics and Autonomous Systems》2010,58(8):991-1002
In this paper we present a new real-time hierarchical (topological/metric) Visual SLAM system focusing on the localization of a vehicle in large-scale outdoor urban environments. It is exclusively based on the visual information provided by a cheap wide-angle stereo camera. Our approach divides the whole map into local sub-maps identified by the so-called fingerprints (vehicle poses). At the sub-map level (low level SLAM), 3D sequential mapping of natural landmarks and the robot location/orientation are obtained using a top-down Bayesian method to model the dynamic behavior. A higher topological level (high level SLAM) based on fingerprints has been added to reduce the global accumulated drift, keeping real-time constraints. Using this hierarchical strategy, we keep the local consistency of the metric sub-maps, by mean of the EKF, and global consistency by using the topological map and the MultiLevel Relaxation (MLR) algorithm. Some experimental results for different large-scale outdoor environments are presented, showing an almost constant processing time. 相似文献
80.
Maria Elena Bruni Francesca Guerriero 《International Transactions in Operational Research》2010,17(2):207-220
The aim of this paper is to investigate the use of heuristic information to efficiently solve to optimality the robust shortest path problem. Starting from the exact algorithm proposed by Murty and Her, we describe how this algorithm can be enhanced by using heuristic rules and evaluation functions to guide the search. The efficiency of the proposed enhanced approach is tested over a range of random generated instances. Our computational results indicate that the use of heuristic criteria is able to speed up considerably the search and that the enhanced exact solution method outperforms the state‐of‐the‐art algorithm proposed by Murty and Her in most of the instances. 相似文献