Solubilization of fish muscle proteins with buffers containing sodium dodecyl sulfate

Summary The extraction of fish muscle protein using SDS containing solubilization buffers was studied varying the time and the temperature of solubilization, as well as pH and SDS concentration of the buffer. At pH < 6 the myofbrillar proteins were incompletely solubilized; temperatures of 80-100 °C resulted in protein degradation observable in the SDS-PAGE.Samples of fish muscle containing high amounts of formaldehyde (50 mmoles FA/kg wet weight) could only be solubilised at 100 °C; on the other hand it was possible to solubilize cooked and/or canned products under mild conditions (2% SDS, 1% 2-ME, pH 8.9, shaking for 2 h at 60 °C).

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Solubilisierung von Fischmuskelproteinen durch Natriumdodecylsulfathaltige Puffer

Zusammenfassung Der Einfluß von Solubilisierungszeit und -temperatur, des pH-Wertes und der SDS-Konzentration des Solubilisierungspuffers auf die Extraktion von Fischmuskelproteinen wurde überprüft. Bei pH-Werten < 6 wurden die myofbrillären Proteine nur unvollständig gelöst; Solubilisierungstemperaturen von 80 bis 100 °C führten zu einem in der SDS-PAGE sichtbaren Proteinabbau. Fischmuskelproben mit hohem Formaldehydgehalt (50 mmole FA/kg Feuchtgewicht) ließen sich nur bei 100 °C vollständig solubilisieren; demgegenüber gelang die weitgehende Solubilisierung gegarter und/oder sterilisierter Produkte unter milden Bedingungen (2% SDS, 1% 2-ME, pH 8,9; 2stündiges Schütteln bei 60 °C).

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Abbreviations FA formaldehyde - IEF isoelectric focusing - 2-ME 2-mercaptoethanol - PA polyacrylamide - PAGE polyacrylamide gel electrophoresis - pI isoelectric point - SDS sodium dodecyl sulfate - TMAO trimethylamine oxide - Tris Tris (hydroxymethyl)methylamine     

3D圆阀塔板性能研究

介绍了3D圆阀塔板的结构特点和实验研究情况。以空气和水为工质进行了冷模实验,研究了3D圆阀塔板的塔板压降、雾沫夹带量和泄漏量等流体力学性能。利用氧解吸法测定其塔板效率,并在相同条件下与F1浮阀塔板进行了对比实验。结果表明,在相同条件下,3D圆阀塔板比传统F1浮阀塔板的塔板效率提高5%~15%,塔板压降约低100 Pa,泄漏量和雾沫夹带量与F1浮阀塔板基本相当,是一种综合性能优良的新型浮阀塔板。     

Automatic labeling and characterization of objects using artificial neural networks

Existing NASA supported scientific databases are usually developed and managed by a team of database administrators whose main concern is the efficiency of the databases in terms of normalization and data search constructs. The populating of the database is usually done in a manual fashion by row and column as the data become available, and the data dictionary is usually defined by the same team (at times with little input from the end science user). This process is tedious, error prone and self-limiting in terms of what can be described in a relational Data Base Management System (DBMS). The next generation Earth remote sensing platforms [i.e., Earth Observing System (EOS)] will be capable of generating data at a rate of over 300 Megabits per second from a suite of instruments designed for different applications. What is needed is an innovative approach that creates object-oriented data-bases that segment, characterize, and catalog, and are manageable in a domain-specific context, and whose contents are available interactively and in near-real-time to the user community. This paper describes work in progress that utilizes an artificial neural net approach to characterize satellite imagery of undefined objects into high-level data objects. The characterized data is then dynamically allocated to an object-oriented database where it can be reviewed and accessed by a user. The definition, development, and evolution of the overall data system model are steps in the creation of an application-driven knowledge-based scientific information system.     

Dynamics of cracked hollow beams

The finite element method with normal quadrilateral plate elements is used for the determination of the bending compliance of orthogonal full and open hollow section beams with transverse cracks. Computed results are compared with those from theory for the full rectangular sections and with experiments performed with rectangular hollow sections. The effect of such cracks on the dynamic response of channel-section beams is investigated. Such analysis is of importance for the identification of cracks in steel structures.     

八钢高炉无料钟炉顶的装料制度

以八钢高炉炼铁实践为依据，系统研究和分析了八钢2号和3号高炉炉顶设备装料制度对高炉冶炼的影响。认为高炉操作制度是当前高炉炼铁的主要技术之一，也是影响高炉焦比主要技术经济指标的重要因素。在原燃料和装备水平一定的条件下，合理的操作制度对高炉的技术经济指标起到决定性的作用。     

大庆油田水平井分流压裂技术

水平井分流压裂的技术原理是将水平井的水平段分成若干个单元，在每个单元的最佳部位进行射孔，每段的射孔数由孔径、单元数、设计施工排量等参数决定，射孔方案为压裂方案的有机组成部分。大庆油田已有5口水平井实施分流压裂，根据油层厚度分为两类：裂缝限制在油层内和裂缝穿透隔层贯穿多个薄油层。通过肇57-平35井和肇57-平33井进行的分流压裂贯穿多个油层现场试验，介绍了大庆油田水平井分流压裂的设计方法、压裂液体系、现场施工控制方法和效果分析方法。微地震裂缝监测结果表明，在最大施工排量7．5m^3／mim条件下，一次施工形成3～4条裂缝，加砂量最大达到75m^3（陶粒），平均砂比达到35％以上。水平井采用分流压裂技术施工后，初期产能可达到相邻直井压裂后产能的2．0倍以上。如何检测裂缝是否穿透了隔层并贯穿多个油层，是指导水平井分流压裂技术的选井选层，提高压裂效果的关键技术。图8表2参2。     

高分辨率双侧向测井响应数值模拟分析

 我国自行研制的新型的高分辨率双侧向测井仪已逐渐投入大规模的生产应用，可为解决油田薄层油气藏的勘探开发问题提供有力的支持。本文利用有限元方法对新型的高分辨率双侧向测井仪及国内目前大规模应用的常规双侧向测井仪的分层能力、探测深度、侵入影响和井眼影响等响应特征进行了数值模拟，并对其测井响应进行了对比分析。数值模拟表明：与常规双侧向测井相比，虽然高分辨率双侧向测井资料井眼的影响更复杂，但测量结果既能得到较高纵向分辨率，又具有较深的径向探测深度，基本上可以取代常规双侧向测井仪在测井中应用。文中数值模拟结果可以为测井作业人员和解释人员在资料验收及解释处理等方面提供参考和指导。     

弱凝胶调驱降维数值模拟方法研究

目前用于弱凝胶调驱的化学驱软件大多存在计算速度慢、数值弥散现象严重的问题,为了提高弱凝胶调驱数值模拟的速度和精度,提出了降低维数的模拟方法。利用追踪流线的方法,将弱凝胶调驱的三维渗流问题,转化为沿流线计算饱和度的一维问题,大大降低了求解饱和度方程组中方程的数目,从而提高了计算速度。利用该方法,模拟了玉门老君庙油田一个试验井区的水驱开发历史,优化了弱凝胶调驱的注入浓度、注入速度及注入体积等参数,并预测了弱凝胶调驱的动态,预测该方案可提高采收率7.6%。     

浍河水库泄洪洞结构优化研究

根据有限元计算原理，结合山西临汾地区浍河水库泄洪洞，对土坝下的大型软基涵洞分层填土施工过程和蓄水对结构的影响进行了模拟计算，得到了更贴近实际的涵洞结构内力和涵洞土压力分布规律，并根据实际地基条件及现有涵洞的两种常见的结构形态对涵洞结构形式进行了优化，其成果已用于设计和施工，证明了该研究方法的科学性和结论的正确性。     

龙桥碾压混凝土双曲拱坝施工质量管理

龙桥水电站碾压混凝土双曲拱坝施工速度快,采取了一系列强化质量管理措施,施工质量优良,重点介绍质量管理的具体做法.