Estimating Thermal Radiation Fields from 3D Flame Reconstruction

The dominant mechanism for the spread of a fire in a building is radiative heat transfer from flames. Therefore, in order to understand how a fire might develop it is necessary to determine the thermal radiation field surrounding a fire. The heat flux at any target location depends upon the flame’s emissive power and the shape factor between the flame’s surface and the target. To determine the shape factor, it is necessary to define the geometry of the flame surface, and develop a method for obtaining the shape factor for that geometry. In this article we describe a method for determining the flame geometry using image-processing techniques. Images of the fire are recorded using two cameras and the flame is reconstructed in three dimensions. Once the surface of the flame has been defined it is possible to calculate the shape factor at a given target. Using the shape factor and an estimate of the emissive power of the flame, the heat flux at the target can be determined. Repeating this process for multiple targets builds a thermal radiation field. Results from applying the method to a controlled flame produced by a diffusion burner show a high correlation between estimated and measured heat flux values.     

Microactuators: Control of the Properties of Micrometer Sized Actuators from Liquid Crystalline Elastomers Prepared in a Microfluidic Setup (Adv. Funct. Mater. 24/2010)

In this article new results on the preparation of monodisperse particles from a liquid crystalline elastomer in a microfluidic setup are presnted. For this, droplets from a liquid crystalline monomer are prepared in a microfluidic device and polymerized while they are flowing inside a microtube. The parti­cles obtained by this method possess an internal orientation, which gives them actuating properties. When they are heated into the isotropic phase of the liquid crystalline material they show a reversible change in shape whereby they change their length in one direction by almost 100%. It is shown how the variation of experimental parameters during their synthesis impacts the properties of these micro‐actuators. Influence over their primal shape, the strength of their shape changing properties, their size, and their mechanical properties is demontrated. From the systematic variation of experimental parameters a deep understanding of the complex processes taking place in a flowing droplet of a liquid crystalline material is obtainted. Additionally NMR analysis and swelling experiments on these actuating materials are provided.     

SQUID-based Technique to Study the Elastic Properties of Solids at Very Low Temperatures

Mechanical oscillators are widely used for measurements of the elastic properties of solids at low temperatures. In most of such experiments mechanical displacements are detected via capacitive changes. In order to improve the sensitivity we have developed a novel detection technique relying on the high sensitivity of a commercial dc SQUID. To demonstrate the performance of the new setup we have measured the variation of the sound velocity and the internal friction of vitreous silica and of the borosilicate glass BK7 in the temperature range from 5 mK to 1 K. Our results agree favorably with former experiments where the displacement was detected capacitively. Furthermore, our results confirm that deviations of the elastic behavior of amorphous solids from the predictions of the standard tunneling model exist at very low temperatures.      

Hitting Below the Bible Belt: The Development of the Gay Rights Movement in Atlanta

Abstract: This study focuses on the gay and lesbian rights movement in America's most conservative region, the South, and its major urban center, Atlanta. Gays and lesbians benefited from a changing political opportunity structure as they overcame severe pressures to develop their own neighborhoods, build a wide range of organizations, and become an important electoral bloc. The movement built upon the city's civil rights legacy and benefited from the dissipation of it opponents, but it has not posed a major threat to what has been labeled Atlanta's regime.     

Long-term interferon-alpha therapy induces autoantibodies against epidermis

Purification of mutant enzymes is a prime requirement of biophysical and biochemical studies. Our investigations on the essential Escherichia coli enzyme glutaminyl-tRNA synthetase demand mutant enzymes free of any wild-type protein contamination. However, as it is not possible to express noncomplementing mutant enzymes in an E. coli glnS-deletion strain, we developed a novel strategy to address these problems. Instead of following the common tactic of epitope-tagging the mutant protein of interest on an extrachromosomal genetic element, we fused a reporter epitope to the 5' end of the chromosomal glnS-gene copy: this is referred to as 'reverse epitope-tagging.' The corresponding strain, E. coli HAPPY101, displays a normal phenotype, and glutaminyl-tRNA synthetase is exclusively present as an epitope-tagged form in cell-free extracts. Here we report the use of E. coli HAPPY101 to express and purify a number of mutant glutaminyl-tRNA synthetases independently of their enzymatic activity. In this process, epitope-tagged wild-type protein is readily separated from mutant enzymes by conventional chromatographic methods. In addition, the absence of wild-type can be monitored by immunodetection using a monoclonal antibody specific for the epitope. The strategy described here for expression and purification of an essential enzyme is not restricted to glutaminyl-tRNA synthetase and should be applicable to any essential enzyme that retains sufficient activity to sustain growth following reverse epitope-tagging.     

Spectral in-situ analysis of NO2, NO3, COD, DOC and TSS in the effluent of a WWTP.

An in-situ UV spectrometer was applied to the effluent of a WWTP in Switzerland and calibrated using a multivariate calibration algorithm based on PLS regression. Except for nitrite, the calibration was based on comparative measurements of the effluent in the plant laboratory. Samples made of stock solution added to three different matrices prepared in the EAWAG laboratory were used for the nitrite calibration because the effluent concentrations were always in the range of 0.06-0.26 mg/l. The results show very good precision for nitrite and nitrate. The measuring range for COD and DOC was not completely covered by the measurements, so the meaningfulness of the results is limited. Nevertheless the precision obtained for soluble COD is high enough for most applications at WWTPs. The accuracy of the TSS measurement is unsatisfactory as regards effluent limits since the spectrometer used does not cover the wavelength region up to 700 nm, which gives better signals for TSS calibration due to its strong correlation with turbidity.     

Experimental study of fire compartment with door opening and roof opening

A series of reduced‐scale experimental fires was conducted to study the characteristics of fire induced vent flows in a reduced‐scale post‐flashover fire compartment with a door opening and a roof opening. The fire source was a heptane pool fire near the wall furthest from the door vent. In the study, the roof vent opening area was systematically varied between experiments and the characteristics of vent flows through the door opening are presented as a function of the roof vent opening area. The experimental results show that the mass flow rate of air into the compartment increases linearly as the size of roof vent opening increases. Analytical vent flow calculations based on the hydrostatic pressure difference between two quiescent environments are presented for a post‐flashover fire compartment with both horizontal and vertical openings. The calculated results are in good agreement with the experimental measurements. Copyright © 2005 John Wiley & Sons, Ltd.     

Nitric oxide synthase expression and function in embryonic and adult cardiomyocytes.

Nitric oxide (NO) is an important signalling molecule that plays a relevant role in different cell systems, among them the adult heart. The effects of NO are primarily mediated through modulation of Ca(2+) homeostasis, myofibrillar contractility, and metabolic regulation in cardiomyocytes. Recent evidence also suggests an important role of NO for cardiomyogenesis by modulating proliferation and differentiation and regulating cardiac function. In the embryonic, but also the healthy and diseased, adult mammalian heart, the inducible (iNOS) and the endothelial (eNOS) nitric oxide synthases (NOS) are detected. However, the expression pattern of NO and its function differ during development. Furthermore, under pathophysiological conditions NOS expression can also change and cause impairment of cardiac performance and cytotoxic effects. The present review focuses on the role and function of NO during cardiomyogenesis, the mechanisms responsible for eNOS availability, and the paracrine effects of NO generated by cardiomyocytes.