Spatio-temporal skin strain distributions evoke low variability spike responses in cuneate neurons

A common method to explore the somatosensory function of the brain is to relate skin stimuli to neurophysiological recordings. However, interaction with the skin involves complex mechanical effects. Variability in mechanically induced spike responses is likely to be due in part to mechanical variability of the transformation of stimuli into spiking patterns in the primary sensors located in the skin. This source of variability greatly hampers detailed investigations of the response of the brain to different types of mechanical stimuli. A novel stimulation technique designed to minimize the uncertainty in the strain distributions induced in the skin was applied to evoke responses in single neurons in the cat. We show that exposure to specific spatio-temporal stimuli induced highly reproducible spike responses in the cells of the cuneate nucleus, which represents the first stage of integration of peripheral inputs to the brain. Using precisely controlled spatio-temporal stimuli, we also show that cuneate neurons, as a whole, were selectively sensitive to the spatial and to the temporal aspects of the stimuli. We conclude that the present skin stimulation technique based on localized differential tractions greatly reduces response variability that is exogenous to the information processing of the brain and hence paves the way for substantially more detailed investigations of the brain''s somatosensory system.     

On the Importance of Being Asymmetric in Stereopsis—Or Why We Should Use Skewed Parallel Cameras

This paper presents a new clever camera sensor, where relative pose determination is not needed, and the sensor is simultaneously capable of using vergence micromovements. Sweeping depth using vergence micromovements promises subpixel depth precision, measuring zero disparity at each time instant. We show that curves preserving zero disparity are exactly conics, nondegenerate or degenerate. Oddly enough, only circles (Vieth-Müller circles) are routinely considered, either theoretically or in practical work, in vergence stereo. Horopters in human vision, cf. Ogle (1932), closely resemble conics.We introduce translational vergence by suggesting the use of a pair of shift-optics CCD cameras. The nonrigidity causes zero disparity curves to become planes, for each fixation. (They are degenerate conics.) We have parallel optical axes, but slanting left and right primary lines of sight. During vergence movements, the primary lines of sight move over time. This has farreaching consequences: Binocular head-eye systems all involve relative camera rotation, to fixate. But, camera rotation is unnecessary. Hence, for relative depth maps, there is no need for measuring camera rotation (relative camera pose) from mechanical sources. Nor are algorithms needed for calculating epipolar lines. The suggested technique removes the need for camera rotations about the optical centers in a binocular head-eye system.     

Supporting management and maintenance of manufacturing knowledge in design automation systems

Many companies base their business strategy on customized products. To enable a high level of product adaptation in an engineer-to-order approach companies invest time and resources to develop design automation systems. Initially, when implementing a design automation system, the focus is on successfully developing a system that generates design variants based on different customer specifications (i.e. the execution of system embedded knowledge and system output). However, in the long run, two important aspects are the management and maintenance of the knowledge that governs the designs. Further, the increasing emphasis on deploying a holistic view of a product’s properties and functions implies an increasing number of life-cycle requirements. The knowledge to adapt the product to fulfil these requirements should also be used and consequently incorporated into the knowledge-base, allowing for correct decisions to be made. In a system for automated variant design, the implications on the product of these life-cycle requirements have to be expressed as algorithms, production rules and/or computational statements to be intertwined with the design calculations. The number of requirements can be significantly large, and the knowledge scattered over different application systems used for the realisation of the design automation system. This makes it difficult to manage and maintain the system as the product life-cycle environment changes and evolves.In this article, the focus is on the requirements related to manufacturing. For that, an approach for the modelling of manufacturing requirements, supporting both knowledge execution and information management, in systems for automated variant design is introduced. The approach has been applied and refined when developing a design automation system in cooperation with a company to demonstrate and verify the approach’s usability.     

Short communication: Recombinant bacteriophage endolysin PlyC is nontoxic and does not alter blood neutrophil oxidative response in lactating dairy cows

Mastitis is the leading cause of antimicrobial use on dairy farms. The potential for antimicrobial resistance has led to the examination of alternative strategies for controlling mastitis. One such alternative is PlyC, a potent peptidoglycan hydrolase derived from the streptococcal C1 bacteriophage that causes targeted lysis of the cell wall of Streptococcus uberis. At a concentration of 1.0 μg/mL, recombinant PlyC can induce lytic activity, suggesting that a low dose may successfully eliminate infection. We evaluated the dose effect of PlyC (1–50 µg/mL) on cytotoxicity and oxidative response on bovine blood polymorphonuclear leukocytes (PMN) obtained from 12 healthy, mid-lactation primiparous dairy cows. Following incubation at 0.5 and 2 h, cytotoxicity was characterized by measuring lactate dehydrogenase release from isolated cells. Oxidative burst response was characterized as the intensity of chemiluminescence produced in the interaction of reactive oxygen species generated in response to 0 or 1.6 µg/mL of phorbol 12-myristate-13-acetate (PMA) with a luminescent substrate with and without addition to PlyC to the incubation matrix. Data were analyzed as a complete randomized block design using mixed model procedures. Cytotoxicity of PlyC was not affected by concentrations up to 50 µg/mL. As expected, PlyC cytotoxicity on PMN varied across incubation time with greater cell toxicity measured at 2 h of incubation as compared with 0.5 h and is primarily attributed to the short life of PMN ex vivo. Concentrations of PlyC up to 50 µg/mL did not affect oxidative response; however, oxidative response was affected by incubation time and PMA concentration. In summary, varying doses of PlyC are nontoxic as estimated by lactate dehydrogenase release from cells and do not appear to alter PMA-stimulated reactive oxygen species production in bovine PMN. These early observations support continued work on the potential for application of this novel agent in combating mastitis.     

The generalizability of the Youth Self-Report syndrome structure in 23 societies.

As a basis for theories of psychopathology, clinical psychology and related disciplines need sound taxonomies that are generalizable across diverse populations. To test the generalizability of a statistically derived 8-syndrome taxonomic model for youth psychopathology, confirmatory factor analyses (CFAs) were performed on the Youth Self-Report (T. M. Achenbach & L. A. Rescorla, 2001) completed by 30,243 youths 11-18 years old from 23 societies. The 8-syndrome taxonomic model met criteria for good fit to the data from each society. This was consistent with findings for the parent-completed Child Behavior Checklist (Achenbach & Rescorla, 2001) and the teacher-completed Teacher's Report Form (Achenbach & Rescorla, 2001) from many societies. Separate CFAs by gender and age group supported the 8-syndrome model for boys and girls and for younger and older youths within individual societies. The findings provide initial support for the taxonomic generalizability of the 8-syndrome model across very diverse societies, both genders, and 2 age groups. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effect of the Heat Treatment on the Chromium Partition in CaO-MgO-SiO2-Cr2O3 Synthetic Slags

Mg-spinel phase is known to be important for control of Cr leaching from Cr-containing slags. The objective of the present study is to get an understanding of the phase relationships in the CaO-MgO-SiO₂-Cr₂O₃ system with a view to control the precipitation of Cr-spinel in the slag phase. The equilibrium phases in CaO-MgO-SiO₂-Cr₂O₃ slag system in the range of 1673 K to 1873 K (1400 °C to 1600 °C) have been investigated experimentally and compared with the results from thermodynamic calculations. The slag compositions close to the industrial slag systems were chosen. The Cr₂O₃ and MgO contents in the slag were fixed to be 6 and 8 wt pct, respectively. The basicity (CaO/SiO₂) of the slag was varied in the range of 1.0 to 2.0. The slags were synthesized at a pre-determined oxygen partial pressure (10^?4) or air (2.13 × 10⁴ Pa) at a temperature above the liquidus temperature. The samples were then soaked at targeted temperatures for 24 hours in controlled atmosphere in order to achieve the equilibrium state before quenching in water. Four different heat-treatment regimes (defined as Ia, Ib, II.a and II.b) in Section II–D) were used in the present experiments. The lower oxygen partial pressure was maintained by a suitable mixture of CO and CO₂ gases. Phases present and their compositions in the quenched slags were studied using scanning electron microscopy coupled with energy-dispersive spectroscopy and X-ray diffraction techniques. The chromium content in the phases present was analyzed using wavelength-dispersive spectrometer. The experimental results obtained are compared with the calculation results from Factsage software. The size of spinel crystals increased drastically after slow-cooling from 1873 K (1600 °C) followed by annealing at 1673 K (1400 °C) for 24 hours (heating regimes II) compared to samples being quenched directly after soaking at 1873 K (1600 °C) (heating regime I.a). It was found that the amount of foreign elements in the spinel phase, and other phases decreased after soaking at oxygen partial pressure of 10^?4 Pa resulting in phases with less defects and foreign oxide contents compared to those treated in air. The size of spinel crystals was found to be larger in samples with lower basicity.     

Cooperation for climate-friendly developments—an analysis of the relationship between the energy and buildings sectors in Sweden

Buildings account for more than 40 % of the total energy demand in the European Union (EU). The energy sector is responsible for 80 % of the total greenhouse gas emissions in the EU, of which more than a third are emitted as a result of energy use in buildings. Given these numbers and the large potential for energy savings in buildings, the energy and buildings sectors emerged as key contributors to fulfilling the European climate targets. Effective cooperation between these two key sectors can contribute significantly to the efficacy of the European climate strategy. However, there may be factors that negatively impact the relationship between the energy and buildings sectors and put cooperation in climate-friendly developments at risk. Based on 23 semi-structured interviews and a web survey answered by key stakeholders, this paper provides a snapshot of the current level of cooperation between the energy and buildings sectors in Sweden and identifies factors that impact the interdependencies between the two sectors. The findings show that the current business models in energy supply and the regulations in place limit the development of mutually beneficial cases between the energy and buildings sectors. This paper contributes to improved knowledge for policymaking that affects both sectors and highlights issues for further study.     

Formation of Amylose‐Lipid Complexes and Effects of Temperature Treatment. Part 1. Monoglycerides

The formation of amylose‐lipid complexes of form I (amorphous structure) and form II (crystalline structure) during heating was studied by differential scanning calorimetry (DSC) for a range of monoglycerides and for monoglyceride mixtures. The temperature treatment applied to amylose‐monoglyceride‐mixtures were either the first scan in DSC (10 °C/min, 15‐144 °C) or a prolonged heat treatment where the samples were kept at 100 °C for 24 h before being analysed in DSC. The temperature treatment influenced which type of complex was formed, and how much, whereas the thermal stability (as judged from the transition peak temperature) was only marginally influenced. It is shown in this study that all the investigated monoglycerides were able to give complex form I as well as complex form II, although the conditions for the formation differed between the monoglycerides. It was found that a simple DSC‐scan was enough for formation of complex form II for the shortest monoglycerides (glycerol monocaprin, glycerol monolaurin and glycerol monomyristin), whereas in case of the longer monoglycerides and monoglyceride mixtures the prolonged heat treatment was required for formation of complex form II. Moreover, the monoglyceride mixtures gave only form II at conditions where the individual components in the mixtures gave both form I and form II.