Human DNA topoisomerase I-mediated cleavages stimulated by ultraviolet light-induced DNA damage

DNA topoisomerases have been proposed as the proteins involved in the formation of the DNA-protein cross-links detected after ultraviolet light (UV) irradiation of cellular DNA. This possibility has been investigated by studying the effects of UV-induced DNA damage on human DNA topoisomerase I action. UV lesions impaired the enzyme's ability to relax negatively supercoiled DNA. Decreased relaxation activity correlated with the stimulation of cleavable complexes. Accumulation of cleavable complexes resulted from blockage of the rejoining step of the cleavage-religation reaction. Mapping of cleavage sites on the pAT153 genome indicated UV-induced cleavage at discrete positions corresponding to sites stimulated also by the topoisomerase I inhibitor camptothecin, except for one. Subsequent analysis at nucleotide level within the sequence encompassing the UV-specific cleavage site revealed the precise positions of sites stimulated by camptothecin with respect to those specific for UV irradiation. Interestingly, one of the UV-stimulated cleavage sites was formed within a sequence that did not contain dimerized pyrimidines, suggesting transmission of the distortion, caused by photodamage to DNA, into the neighboring sequences. These results support the proposal that DNA structural alterations induced by UV lesions can be sufficient stimulus to induce cross-linking of topoisomerase I to cellular DNA.     

Assessing the health research’s social impact: a systematic review

Scientometrics - In recent year, a growing attention is dedicated to the assessment of research’s social impact. While prior research has often dealt with results of research, the last decade...     

Influence of Surface Morphology on the Torsion Fracture of NiTi Endodontic Instruments

This study analyzed the influences of manufacturing process (twisting and machining) and surface finishing on the angular distortion, maximum torque, and toughness of two NiTi endodontic instruments (Race—FKG Dentaire and TF—Twisted Files, SybronEndo). Statistical analysis of results showed that TF instruments (twisted) had higher distortion angle (p < 0.05) and lower maximum torque to fracture (p < 0.05) than Race instruments (machined). SEM analysis of fractured instruments showed a surface morphology characteristic of ductile fracture, with plastic deformation in the helical shafts. The results suggest that both instruments are equally suitable for clinical applications. This study is purely scientific as it evaluates only some material properties, and is not intended to endorse a commercial product.     

Identification of unbalance forces by metaheuristic search algorithms

This paper discusses the identification of parameters in rotary systems, namely, the unbalance magnitude, phase and position in the rotor system. These parameters can be identified using the measured orbits in the hydrodynamic bearings. The oil film forces are evaluated in the different positions of the orbit of the journal and are applied to the model of the shaft. The model, integrated in time domain, allows with an assumed unbalance, to simulate the orbits. The objective function is basically the difference between measured and simulated orbits, and its minimum corresponds to the identified unbalance amount, phase and position along the shaft. With respect to traditional model based identification procedures, this approach using oil film forces instead of oil film linearized stiffness and damping coefficients, and unfiltered orbits instead of 1X vibration components is suitable to deal with non-linear behaviour of the system.     

Highly Sensitive Pulsed Digital Holography for Built-in Defect Analysis with a Laser Excitation

A highly sensitive method is presented for noninvasive defect analysis on thin structures with a Q-switched double-pulsed ruby laser with frequency doubling (347 nm). In our research we feature an all-optical arrangement, where a focused laser pulse derived from the same ruby laser (694 nm) acts as a built-in synchronous excitation source for digital holographic interferometry. The recordings are made with a CCD camera for capturing two holograms (two states of the specimen) corresponding to the two UV laser pulses with a short time separation (10-50 mus). Subtraction of the phase distribution in two digital holograms gives a fringe phase map that shows the change in deformation of the specimen between the recordings. The advantage of the proposed method is two fold. First, the use of a shorter wavelength results in a higher sensitivity. Second, owing to the induced synchronous built-in optical excitation, the specimen is not subjected to any external physical excitation devices. Experimental results are presented on identification and evaluation of defects in thin metal sheets.     

Historiography of psychology in Spain: The last decade.

History of Psychology has been an extremely active field for many years in Spain. Both the great expansion of psychological studies and the inclusion of history as a compulsory subject in the psychology curriculum are crucial factors helping to understand the current state of affairs. The aim of this paper is to provide a survey of the work done in this area over the last decade, covering research, teaching, and institutional developments. The study includes consideration of aspects such as main research lines, major theoretical and methodological trends, teaching materials, relevant archives and exhibits, and significant websites. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Meshless Helmholtz-Hodge Decomposition

Vector fields analysis traditionally distinguishes conservative (curl-free) from mass preserving (divergence-free) components. The Helmholtz-Hodge decomposition allows separating any vector field into the sum of three uniquely defined components: curl free, divergence free and harmonic. This decomposition is usually achieved by using mesh-based methods such as finite differences or finite elements. This work presents a new meshless approach to the Helmholtz-Hodge decomposition for the analysis of 2D discrete vector fields. It embeds into the SPH particle-based framework. The proposed method is efficient and can be applied to extract features from a 2D discrete vector field and to multiphase fluid flow simulation to ensure incompressibility.     

Intrusion Detection System for Wireless Sensor Networks Using Danger Theory Immune-Inspired Techniques

An IDS framework inspired in the Human Immune System to be applied in the wireless sensor network context is proposed. It uses an improved decentralized and customized version of the Dendritic Cell Algorithm, which allows nodes to monitor their neighborhood and collaborate to identify an intruder. The work was implemented and tested both in simulation and in real sensor platform scenarios, comparing them to each other and was also compared to a Negative Selection Theory implementation in order to demonstrate its efficiency in detecting a denial-of-sleep attack and in energy consumption. Results demonstrated the success of the proposal.