The chicken beta-globin gene promoter forms a novel "cinched" tetrahelical structure

We have previously shown that the G-rich sequence G16CG(GGT)2GG in the promoter region of the chicken beta-globin gene poses a formidable barrier to DNA synthesis in vitro (Woodford et al., 1994, J. Biol. Chem. 269, 27029-27035). The K+ requirement, template-strand specificity, template concentration independence, and involvement of Hoogsteen bonding suggested that the underlying basis of this new type of DNA synthesis arrest site might be an intrastrand tetrahelical structure. However, the arrest site lacks the four G-rich repeats that are a hallmark of previously described intramolecular tetraplexes and contains a number of noncanonical bases that would be expected to greatly destabilize such a structure. Here we report evidence for an unusual K+-dependent intrastrand "cinched" tetraplex. This structure has several unique features including the incorporation of bases other than guanine into the stem of the tetraplex, interaction between loop bases and bases in the flanking region, and base pairing between bases 3 and 5 of the tetrahelix-forming region to form a molecular "cinch." This finding extends the range of sequences capable of tetraplex formation as well as our appreciation of the conformational complexity of the chicken beta-globin promoter.     

Auditory-visual cross-modal perception of communicative stimuli in Tufted Capuchin Monkeys (Cebus apella).

Research on cross-modal performance in nonhuman primates is limited to a small number of sensory modalities and testing methods. To broaden the scope of this research, the authors tested capuchin monkeys (Cebus apella) for a seldom-studied cross-modal capacity in nonhuman primates, auditory-visual recognition. Monkeys were simultaneously played 2 video recordings of a face producing different vocalizations and a sound recording of 1 of the vocalizations. Stimulus sets varied from naturally occurring conspecific vocalizations to experimentally controlled human speech stimuli. The authors found that monkeys preferred to view face recordings that matched presented vocal stimuli. Their preference did not differ significantly across stimulus species or other stimulus features. However, the reliability of the latter set of results may have been limited by sample size. From these results, the authors concluded that capuchin monkeys exhibit auditory-visual cross-modal perception of conspecific vocalizations. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The screen printing of a power-law fluid

We present a two-dimensional large-aspect-ratio model for the off-contact screen printing of a power-law fluid. We extend the work of White et al. (J Eng Math 54:49–70, 2005) by explicitly including the fluid/air free surface that is present beneath the screen ahead of the squeegee. In the distinguished parameter limit of greatest interest to industry, the process is quasi-steady on the time-scale of a print and can be analysed in three separate regions using the method of matched asymptotic expansions. This allows us to predict where the fluid transfers through the screen, the point at which it first makes contact with the substrate, and the amount of fluid deposited on the substrate during a print stroke. Finally, we show that using a shear-thinning fluid will decrease the amount of fluid transferred ahead of the squeegee, but increase the amount of fluid deposited on the substrate.     

Sr2−XLaXMgMoO6 and Sr2−XLaXMgNbO6 for Use as Sulfur‐Tolerant Anodes Without a Buffer Layer

The objective of this effort is to synthesize and characterize a series of lanthanum‐(La) doped Sr₂MgMoO₆ (SMMO) and La‐doped Sr₂MgNbO₆ (SMNO) anode materials which can be used in combination with lanthanum‐containing electrolytes to mitigate the effects of lanthanum poisoning in solid oxide fuel cells (SOFCs). Currently, an La_0.4Ce_0.6O_1.8 (LDC) buffer layer is used with many perovskite‐based anode materials to prevent La diffusion into the anode from the La_0.8Sr_0.2Ga_0.8Mg_0.2O_2.8 (LSGM) electrolyte which can create a resistive La species that impedes electrochemical performance. The LDC buffer layer, with diminished electronic conductivity, adds an extra level of complexity in the SOFC manufacturing process. Further, this extraneous layer presents an added experimental challenge when assessing anode material performance. Overall electrochemical performance could be improved if the resistive buffer layer could be removed, thereby allowing the anode material to have direct contact with the electrolyte. To accomplish this, a new class of anode materials was synthesized with the goal of balancing “La” chemical potential between these neighboring materials. La‐doped SMMO and SMNO were prepared and studied. It was hypothesized that by incorporating La into the anode, the gradient of chemical activity between the anode and electrolyte would decrease, which would prevent La diffusion. These anode materials were synthesized via a sol–gel methodology and characterized with X‐ray diffraction to assess phase purity. The conductivity of the materials was analyzed in the presence of both H₂ and 100 ppm H₂S/H₂ to determine the stability and performance of these materials during device operation. The stability experiments demonstrated that 40% La‐doped SMNO is stable in all pertinent environments while not reacting with the LSGM electrolyte.     

Symmetric Equations for Evaluating Maximum Torsion Stress of Rectangular Beams in Compliant Mechanisms

There are several design equations available for calculating the torsional compliance and the maximum torsion stress of a rectangular cross-section beam, but most depend on the relative magnitude of the two dimensions of the crosssection(i.e., the thickness and the width). After reviewing the available equations, two thickness-to-width ratio Independent equations that are symmetric with respect to the two dimensions are obtained for evaluating the maximum torsion stress of rectangular cross-section beams. Based on the resulting equations, outside lamina emergent torsional joints are analyzed and some useful design Insights are obtained. These equations, together with the previous work on symmetric equations for calculating torsional compliance, provide a convenient and effective way for designing and optimizing torsional beams in compliant mechanisms.     

Non-curated distributed databases for experimental data and models in neuroscience

Neuroscience is generating vast amounts of highly diverse data which is of potential interest to researchers beyond the laboratories in which it is collected. In particular, quantitative neuroanatomical data is relevant to a wide variety of areas, including studies of development, aging, pathology and in biophysically oriented computational modelling. Moreover, the relatively discrete and well-defined nature of the data make it an ideal application for developing systems designed to facilitate data archiving, sharing and reuse. At present, the only widely used forms of dissemination are figures and tables in published papers which suffer from inaccessibility and the loss of machine readability. They may also present only an averaged or otherwise selected subset of the available data. Numerous database projects are in progress to address these shortcomings. They employ a variety of architectures and philosophies, each with its own merits and disadvantages. One axis on which they may be distinguished is the degree of top-down control, or curation, involved in data entry. Here we consider one extreme of this scale in which there is no curation, minimal standardization and a wide degree of freedom in the form of records used to document data. Such a scheme has advantages in the ease of database creation and in the equitable assignment of perceived intellectual property by keeping the control of data in the hands of the experts who collected it. It does, however, require a more sophisticated infrastructure than conventional databases since the software must be capable of organizing diverse and differently documented data sets in an effective way. Several components of a software system to provide this infrastructure are now in place. Examples are presented, showing how these tools can be used to archive and publish neuronal morphology data, and how they can give an integrated view of data stored at many different sites.