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General-base catalysis in the active site of serine proteasesis carried out by the imidazole side chain of a histidine. Duringformation of the transition state, an adjacent carboxylic acidgroup stabilizes the positive charge that forms on the general-basecatalyst and as a result contributes several orders of magnitudeto the catalytic efficiency of these enzymes. In the recentlydiscovered family of self-cleaving proteins exemplified by theLexA repressor of Escherichia coli, instead of the imidazoleof a histidine, the active-site general-base catalyst was foundto be the -amino of a lysine. The considerably higher capacityof the lysine side chain for proton acceptance raises interestingquestions concerning the role of electrostatic interactionsin the mechanism of proton transfer by this highly basic group.The negative charge elimination studies described here and theireffects on the kmax and pK of LexA self-cleavage are consistentwith a model in which electrostatic interactions between anacidic side chain and the general-base catalyst form a barrierto proton transfer. The implications are that the -amino group,unlike the imidazole group, is capable of effecting proton transferwithout the intervention of a countercharge. 相似文献
33.
Huy Minh Dao Amit Raviraj Pillai Rishi Thakkar Sanjiv Parajuli Esteban Urena-Benavides Seongbong Jo 《应用聚合物科学杂志》2021,138(2):49665
Light-sensitive drug delivery systems are considered ideal for applications in the biomedical fields for their ability to release the payload in an on-demand spatiotemporal controlled manner through the manipulation of the light source. Among the broad radiation spectrum, near infrared (NIR) light is considered advantageous compared to UV and visible light, due to its inherently lower photodamage to normal tissues and deeper penetration to lesion areas. In this study, we report a successful synthesis of a polymer capable of undergoing partial degradation upon irradiation with NIR light by conjugating 10-N-carbamoyl linkage methylene blue (MB) moiety, a NIR photocleavable ligand, with polyethylene glycol (PEG). Through effective coupling of MB, a hydrophobic moiety, to the hydrophilic PEG molecule, an amphiphilic polymer was synthesized, as demonstrated by a lowered surface tension (55 mN/m at 0.1% wt/vol). Subsequently, photo-induced reversal of surface activity associated with self-assembled structure disruption, was displayed by surface tension measurements, size distribution analysis, and burst release profile of paclitaxel (PTX) from polymeric micelles upon the exposure to NIR irradiation. 相似文献
34.
Tuan Anh Tran In Seop Na Soo Hyung Kim 《International Journal on Document Analysis and Recognition》2016,19(3):191-209
Document layout analysis or page segmentation is the task of decomposing document images into many different regions such as texts, images, separators, and tables. It is still a challenging problem due to the variety of document layouts. In this paper, we propose a novel hybrid method, which includes three main stages to deal with this problem. In the first stage, the text and non-text elements are classified by using minimum homogeneity algorithm. This method is the combination of connected component analysis and multilevel homogeneity structure. Then, in the second stage, a new homogeneity structure is combined with an adaptive mathematical morphology in the text document to get a set of text regions. Besides, on the non-text document, further classification of non-text elements is applied to get separator regions, table regions, image regions, etc. The final stage, in refinement region and noise detection process, all regions both in the text document and non-text document are refined to eliminate noises and get the geometric layout of each region. The proposed method has been tested with the dataset of ICDAR2009 page segmentation competition and many other databases with different languages. The results of these tests showed that our proposed method achieves a higher accuracy compared to other methods. This proves the effectiveness and superiority of our method. 相似文献
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In this paper, we introduce an internet voting protocol which satisfies desired security requirements of electronic voting. In the newly proposed protocol, we allow the adversaries to get more power than in any previous works. They can be coercers or vote buyers outside, and corrupted parties inside our system. These adversaries also have ability to collude with each other to ruin the whole system. Our main contribution is to design an internet voting protocol which is unsusceptible to most of sophisticated attacks. We employ the blind signature technique and the dynamic ballots instead of complex cryptographic techniques to preserve privacy in electronic voting. Moreover, we also aim at the practical system by improving the blind signature scheme and removing physical assumptions which have often been used in the previous works. 相似文献
37.
Development of a quadruped walking robot AiDIN-III using biologically inspired kinematic analysis 总被引:1,自引:0,他引:1
Ig Mo Koo Duc Trong Tran Yoon Haeng Lee Hyungpil Moon Ja Choon Koo Sangdeok Park Hyouk Ryeol Choi 《International Journal of Control, Automation and Systems》2013,11(6):1276-1289
This paper presents a bio-inspired mechanism design for a quadruped walking robot. The approach is derived from the observation on the behaviors of quadruped locomotion, skeletal structure, and the study on the stability of walking based on morphological analysis. In the first, we define the design parameters such as the dimensions of the body and limbs, the center of mass position, and locomotion mechanisms based on surveys on the literatures from biologists. Then, by using the parameters, we propose an useful framework for determining the design parameters of a quadruped walking robot. For implementations, we manufacture a dog-type self-contained quadruped walking robot, named AiDIN-III (Artificial Digitigrade for Natural Environment version III) and the effectiveness of the proposed idea is validated via experimental works. 相似文献
38.
OBJECTIVE: Muscle activity with and without the use of commercially available patient assistive devices during bed rising and lowering was quantified. BACKGROUND: Limited research is available in understanding or evaluating the physical benefits of assistive devices for patient use following major abdominal surgery. METHODS: Twenty healthy participants (9 men, 11 women) took part in a laboratory study to test the effects of device configuration (five levels) and bed elevation angle (0 degree and 30 degrees) on mean and peak upper and lower rectus abdominis and external oblique concentric and eccentric muscle activity. RESULTS: Reduced muscle activity was associated with the use of an assistive device, as compared with manual bed rising (unassisted). Positioning the devices at a higher anchor height and/or increasing the bed elevation angle further reduced muscle activity. Objective and subjective differences between the two assistive devices evaluated in the study were found. CONCLUSION: These results suggest that self-assistive devices may speed recovery because of reduced loads on damaged tissues. APPLICATION: Potential applications of this research include the assessment of other commercially available lift aids or comparisons of self-assistive lift aids with hospital-housed lift aids used to speed recovery rates. 相似文献
39.
We study the unsteady pulsatile flow of blood in an artery, where the effects of body acceleration are included. The blood is modeled as a modified second-grade fluid where the viscosity and the normal stress coefficients depend on the shear rate. It is assumed that the blood near the wall behaves as a Newtonian fluid, and in the core as a non-Newtonian fluid. This phenomenon is also known as the Fahraeus–Lindqvist effect. The equations are made dimensionless and solved numerically. 相似文献
40.
Semplore: A scalable IR approach to search the Web of Data 总被引:1,自引:0,他引:1
Haofen Wang Qiaoling Liu Thomas Penin Linyun Fu Lei Zhang Thanh Tran Yong Yu Yue Pan 《Journal of Web Semantics》2009,7(3):177-188
The Web of Data keeps growing rapidly. However, the full exploitation of this large amount of structured data faces numerous challenges like usability, scalability, imprecise information needs and data change. We present Semplore, an IR-based system that aims at addressing these issues. Semplore supports intuitive faceted search and complex queries both on text and structured data. It combines imprecise keyword search and precise structured query in a unified ranking scheme. Scalable query processing is supported by leveraging inverted indexes traditionally used in IR systems. This is combined with a novel block-based index structure to support efficient index update when data changes. The experimental results show that Semplore is an efficient and effective system for searching the Web of Data and can be used as a basic infrastructure for Web-scale Semantic Web search engines. 相似文献