Phenotypic Characterization and Genotypic Subtyping of Salmonella enterica Serovars Enteritidis and Gallinarum Isolated from Human and Poultry-Related Samples

This study aimed to assess the serotypes, antibiotic susceptibility, and molecular subtyping of Salmonella enterica isolated from food products and human patients with gastroenteritis. A total of 59 isolates were investigated, and the results revealed a predominance of S. Enteritidis with 57.63% (34/59) S. Gallinarum held second with 15.62% (5/32) of total food borne. While, isolates from humans showed 18.51% (5/27) of S. Typhimurium. High level of resistance to nalidixic acid was noted among food strains and 35.29% of human isolates were found to be multidrug resistant. Eight representative isolates were subtyping using three molecular approaches, ribotyping, Multilocus sequence typing (MLST) and Multiple-locus variable number tandem repeat analysis (MLVA). MLST showed three sequence types corresponding to two clonal complexes, (ST-78, CC-4) for S. Gallinarum, (ST-11, CC-4) for S. Enteritidis and (S-367, CC-37) for S. Cerro. While, MLVA generated six different profiles targeting nine loci for S. Enteritidis and S. Gallinarum.     

Hypoglycaemia‐free artificial pancreas project

Driving blood glycaemia from hyperglycaemia to euglycaemia as fast as possible while avoiding hypoglycaemia is a major problem for decades for type‐1 diabetes and is solved in this study. A control algorithm is designed that guaranties hypoglycaemia avoidance for the first time both from the theory of positive systems point of view and from the most pragmatic clinical practice. The solution consists of a state feedback control law that computes the required hyperglycaemia correction bolus in real‐time to safely steer glycaemia to the target. A rigorous proof is given that shows that the control‐law respects the positivity of the control and of the glucose concentration error: as a result, no hypoglycaemic episode occurs. The so‐called hypo‐free strategy control is tested with all the UVA/Padova T1DM simulator patients (i.e. ten adults, ten adolescents, and ten children) during a fasting‐night scenario and in a hybrid closed‐loop scenario including three meals. The theoretical results are assessed by the simulations on a large cohort of virtual patients and encourage clinical trials.Inspec keywords: biochemistry, medical control systems, blood, diseases, medical computing, closed loop systems, biomedical equipment, state feedback, patient treatment, patient monitoring, biomedical measurement, physiological models, sugarOther keywords: fasting‐night scenario, hybrid closed‐loop scenario, hypoglycaemia‐free artificial pancreas project, blood glycaemia, euglycaemia, type‐1 diabetes, control algorithm, guaranties hypoglycaemia avoidance, pragmatic clinical practice, state feedback control law, required hyperglycaemia correction bolus, rigorous proof, control‐law, glucose concentration error, hypo‐free strategy control     

Testability of parity checkers

Checkers are used in digital circuits to detect both intermittent and stuck-at faults. The most common error detectors are parity checkers. Such circuits are themselves subject to failures. The use of parity trees is outlined, and techniques for testing them are surveyed. The effect of the checker's structure on its testability is discussed. Several fault models are considered: single stuck-at, multiple stuck-at, and bridging faults. The effectiveness of single stuck-at fault test sets in detecting multiple stuck-at and bridging faults is described. Upper bounds for the double fault coverage of the minimal single fault test are given for different tree structures. The testabilities of some selected checkers are examined to illustrate the concepts developed. A built-in self-test is proposed     

A scheme for finite element analysis of mode I and mixed mode stable crack growth and a case study with AISI 4340 steel

A new scheme for elastic–plastic finite element analysis has been proposed for the study of stable crack growth (SCG) from initiation to instability in both mode I and mixed modes (I and II). The scheme is based on node-release technique and helps to determine the variation of fracture load with crack extension without requiring much computer storage and time. The scheme permits predictions of load variation with load line displacement (LLD), maximum fracture load, crack tip current plastic zone and crack edge profile. In the analysis the condition for crack extension at every stage of the SCG is considered to be governed by CTOA/COD reaching a critical value. The scheme of analysis is different from the ones proposed by earlier investigators. The whole SCG is analysed in a few stages using the ANSYS software and a single discretization. Element arrangement in the discretization is decided from the very beginning; it has a capability of accommodating changes in boundary conditions arising out of crack extension in the later stages. Each stage is analysed afresh ignoring state of stress–strain reached at a material point at the end of the previous stage. Case studies on both mode I and mixed mode presented considering AISI 4340 steel, which is widely used in nuclear power industry, indicate that the SCG through it can be characterized in terms of a single COD or CTOA. Predictions for the initiation and maximum fracture loads in both the cases compare very closely with the experimental data reported. The results presented also include the value of critical COD/CTOA (0.035 mm/0.0875 rad) characterizing the SCG through the steel and show that the initiation load is not significantly affected by crack tip radius up to 0.05 mm.     

Performance of Low Earth Orbit Satellite Systems with Coding

The effect of block, convolutional and Turbo coding on the probability of error and the capacity are investigated for CDMA Low Earth Orbit (LEO) Satellite systems. The model employed assumes a contaminated Gaussian traffic model to be more compatible with different population areas. The conventional Gaussian distribution can be considered as a special case.     

A framework for the characterization and verification of embedded phase-locked loops

With the increasing use of phase-locked loops (PLLs) embedded in FPGAs, ASICs, and system-on-chip (SOC), there is a growing need for methods to verify their operation. This paper describes a general and organized list that includes tests for lock, jitter, stability, and modulation response. The list is offered as a guide to the verification and testing of an embedded PLL. For that reason, it is presented in such a way as to allow the reader to determine the extent to which the verification will be carried out. The tests are covered from the easiest to the most complicated to perform, with the amount of information gathered increasing along with the complexity of the test.     

Influence of state of stress on mixed mode stable crack growth through D16AT aluminium alloy

The stable crack growth through three-point bend (TPB) and stiffened and unstiffened compact tension (CT) specimens of D16AT aluminium alloy has been studied both theoretically and experimentally. The specimen thickness is 8 mm. The variation of load with crack opening displacement, the extent of stable crack growth, the cumulative plastically deformed zone and crack edge profiles have been obtained experimentally. These are also predicted theoretically under the assumption of either a state of plane stress or plane strain using a finite element scheme and the COA criterion. Generally, the experimental results agree well with the predictions based on the plane stress condition. There appears to be no significant variation in size of the experimental cumulative plastic zone across the specimen thickness, thereby indicating that the constraint on the plastic zone does not develop near the mid-thickness region.     

Synthesis and characterization of cellulose triacetate and poly(ethylene imine) membranes containing a polyether macrobicyclic: Their application to the separation of copper(II) and silver(I) ions

A cellulose triacetate (CTA)–poly(ethylene imine) (PEI) membrane containing a polyether macrobicyclic (cryptand) as a carrier and 2‐nitrophenyloctyl ether (NPOE) as a plasticizer was prepared. The CTA–PEI–cryptand–NPOE membrane was characterized with chemical techniques as well as Fourier transform infrared, X‐ray diffraction, differential scanning calorimetry, and scanning electron microscopy. The permeation of silver and copper ions through the CTA–PEI–cryptand–NPOE membrane was comparable to that through a supported liquid membrane. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 93: 1401–1410, 2004     

A Dempster–Shafer Based Tit-for-Tat Strategy to Regulate the Cooperation in VANET Using QoS-OLSR Protocol

In this paper, we address the problem of cooperation among vehicles in VANET using QoS-OLSR protocol in the presence of selfish nodes. QoS-OLSR is a proactive protocol that considers the Quality of Service (QoS) of the nodes while electing the cluster-heads and selecting the Multi-Point Relay (MPRs) nodes. Cluster-heads and MPRs might misbehave on the roads by over-speeding or under-speeding. Classical and generous Tit-for-Tats are proposed to analyze the interaction among vehicles. However, both strategies are not able to enforce the cooperation due the fact that they (1) count on individual watchdogs monitoring, (2) rely on the node-to-node cooperation decision, (3) and ignore the high mobility and packet collisions. Therefore, we propose a Dempster–Shafer based Tit-for-Tat strategy that is able to improve the decision and regulate the cooperation in the vehicular network. This is done by (1) launching a cooperative watchdogs monitoring, (2) correlating the observations of the different watchdogs using Dempster–Shafer theory, and (3) propagating the decisions among clusters. Thereafter, we compare the Dempster–Shafer based strategy with several strategies derived from the original Tit-for-Tat. Simulation results prove that the Dempster–Shafer based strategy is able to maintain the survivability of the vehicular network in the presence of high mobility and packet collisions with minimal time and overhead.     

Mesure de coefficients de convection par méthode impulsionnelle—influence de la perturbation de la couche limiteMeasurement of the convective heat coefficients by the pulsed method—influence of the boundary-layer perturbation

We present here some elements in order to improve the non-destructive procedures for the measurement of the local heat transfer coefficient between a plate uniformly heated and an air-flow. We use the pulsed photothermal method which consists of analysing the transient temperature on the front face of a wall, initially subjected to a convective flow, after a sudden deposit of luminous energy. For the examination of the experimental thermograms, two models are used: in the first one the heat coefficient is assumed to remain constant during the pulsed experiment, and we take into account its variation in time in the second one. The temporal variation law of the heat transfer coefficient used in the second model is obtained by extension of the differential method to the unsteady laminar boundary-layer due to a finite duration excitation. We compare the results of the two models in transient state with those given in steady state by multi-layered heat flux sensor.