Influence of moisture content on the specific methanogenic activity of dry mesophilic municipal solid waste digestate

The objective of this study was to evaluate the influence of moisture content on the specific methanogenic activity (SMA) of a fresh dry mesophilic digestate from a municipal solid waste digester plant. For this purpose, SMA tests were performed under mesophilic conditions in 500 mL glass bottles of volume used as batch reactors, during a period of 20–25 days. Cellulose, propionate and acetate were used as substrates (5 g_COD kg^?1 digestate) at four different moisture contents, ranging from 65 to 82%. The moisture content strongly influenced the specific methanogenic activity. The highest SMA values were observed at a moisture content of 82% (11.1, 7.8 and 6.0 mg_COD g_VS^?1 d^?1 for cellulose, propionate and acetate spikes, respectively). SMA and moisture content were found to be linearly linked. Dry digestion at low water content is thus detrimental to the biological activity, probably due to physical limitations. Copyright © 2011 Society of Chemical Industry     

Phase-sensitive spatially-modulated surface plasmon resonance polarimetry for detection of biomolecular interactions

We here describe a scheme of spatially modulated surface plasmon resonance (SPR) polarimetry that enables to combine ultra-high phase sensitivity with good signal-to-noise background. The proposed approach uses spatial modulation of s-polarized component by birefringent elements and the extraction of phase-polarization information by Fourier-transform methods. This scheme was tested for monitoring the interactions between an antibody and its biological partner. Our experimental data, collected by amplitude-sensitive and phase-sensitive polarimetry demonstrate that the latter scheme provides at least one order of magnitude improvement in terms of detection limit.     

One Question,Multiple Answers: Biochemical and Biophysical Screening Methods Retrieve Deviating Fragment Hit Lists

Fragment‐based lead discovery is gaining momentum in drug development. Typically, a hierarchical cascade of several screening techniques is consulted to identify fragment hits which are then analyzed by crystallography. Because crystal structures with bound fragments are essential for the subsequent hit‐to‐lead‐to‐drug optimization, the screening process should distinguish reliably between binders and non‐binders. We therefore investigated whether different screening methods would reveal similar collections of putative binders. First we used a biochemical assay to identify fragments that bind to endothiapepsin, a surrogate for disease‐relevant aspartic proteases. In a comprehensive screening approach, we then evaluated our 361‐entry library by using a reporter‐displacement assay, saturation‐transfer difference NMR, native mass spectrometry, thermophoresis, and a thermal shift assay. While the combined results of these screening methods retrieve 10 of the 11 crystal structures originally predicted by the biochemical assay, the mutual overlap of individual hit lists is surprisingly low, highlighting that each technique operates on different biophysical principles and conditions.     

Facial animation retargeting and control based on a human appearance space

Expressive facial animations are essential to enhance the realism and the credibility of virtual characters. Parameter‐based animation methods offer a precise control over facial configurations while performance‐based animation benefits from the naturalness of captured human motion. In this paper, we propose an animation system that gathers the advantages of both approaches. By analyzing a database of facial motion, we create the human appearance space. The appearance space provides a coherent and continuous parameterization of human facial movements, while encapsulating the coherence of real facial deformations. We present a method to optimally construct an analogous appearance face for a synthetic character. The link between both appearance spaces makes it possible to retarget facial animation on a synthetic face from a video source. Moreover, the topological characteristics of the appearance space allow us to detect the principal variation patterns of a face and automatically reorganize them on a low‐dimensional control space. The control space acts as an interactive user‐interface to manipulate the facial expressions of any synthetic face. This interface makes it simple and intuitive to generate still facial configurations for keyframe animation, as well as complete temporal sequences of facial movements. The resulting animations combine the flexibility of a parameter‐based system and the realism of real human motion. Copyright © 2010 John Wiley & Sons, Ltd.     

Autonomic Self‐Repairing Glassy Materials

A new process that enables glassy materials to self‐repair from mechanical damage is presented in this paper. Contrary to intrinsic self‐healing, which involves overheating to enable crack healing by glass softening, this process is based on an extrinsic effect produced by vanadium boride (VB) particles dispersed within the glass matrix. Self‐repair is obtained through the oxidation of VB particles, and thus without the need to increase the operating temperature. The VB healing agent is selected for its capacity to oxidize at a lower temperature than the softening point of the glass. Thermogravimetric analyses indeed show that VB oxidation is rapid and occurs below the glass transition temperature. Solid‐state nuclear magnetic resonance spectroscopy indicates that VB is oxidized into V₂O₅ and B₂O₃, which enable the local formation of glass. The autonomic self‐healing effect is demonstrated by an in situ experiment visualized using an environmental scanning electron microscope. It is shown that a crack could be healed by the VB oxidation products.     

X-ray microbeam strain investigation on Cu-MEMS structures

The mechanical properties of simple metals in small dimensions have been the objects of many studies. Average stress measurements clearly show that yield stress increases significantly when the size decreases but local strain measurements indicate considerable grain-to-grain and intra-grain strain variations. The link between the macroscopic behaviour of microstructures and the corresponding local processes is still difficult to analyse.Copper is used in advanced microelectronic interconnects and the mechanical properties of narrow interconnects is a key issue for the reliability of devices. Average strain measurements performed on line arrays show a drastically different behaviour in narrow lines as compared with wide lines. ST microelectronics in Rousset developed a MEMS sensor integrated in the damascene process to follow the stress after processing. This microsystem can be a very efficient tool to monitor the average stress in interconnects on the chip. The gradient of strain in interconnects may lead to failures from void and hillocks and local stress repartition can be obtained through X-ray microdiffraction. Laue microdiffration is a unique non-destructive method to investigate the strain grain-by-grain in microstructures. The diffraction patterns were obtained at ESRF on BM32 beam line. We present the first steps of the investigation of a MEMS investigated by Laue microdiffraction. The sub-micron X-ray-beam allows a mapping of the microstructure with a step of around 1 μm, which is also the value of the estimated grain size. We present results on the orientation and stress at a sub-micron scale obtained from the Laue-diffraction measurements on the MEMS.     

ATRP with Alkyl Pseudohalides Acting as Initiators and Chain Transfer Agents: When ATRP and RAFT Polymerization Become One

All controlled radical polymerization (CRP) procedures rely on a dynamic and rapid equilibrium between dormant and active species. This equilibrium can be established through different mechanisms and all the resulting CRP processes have their own advantages and limitations. Therefore, it becomes interesting to investigate the possibility of combining CRP techniques to eliminate specific drawbacks of each individual procedure. Atom transfer radical polymerization (ATRP) with alkyl pseudohalides acting as initiators and chain transfer agents was developed for that purpose. The process relies on a dual mechanism involving both activation deactivation and reversible addition fragmentation chain transfer (RAFT). This peculiar characteristic of ATRP with alkyl pseudohalides acting as chain transfer agents made it possible to overcome some of the limitations typically associated with conventional ATRP and RAFT polymerization as well as to prepare new responsive materials.     

Efficient Implementations of Software Architectures via Partial Evaluation

The notion of flexibility (that is, the ability to adapt to changing requirements or execution contexts) is recognized as a key concern in structuring software, and many architectures have been designed to that effect. However, the corresponding implementations often come with performance and code size overheads. The source of inefficiency can be identified to be in the loose integration of components, because flexibility is often present not only at the design level but also in the implementation. To solve this flexibility vs. efficiency dilemma, we advocate the use of partial evaluation, which is an automated technique to produce efficient, specialized instances of generic programs. As supporting case studies, we consider several flexible mechanisms commonly found in software architectures: selective broadcast, pattern matching, interpreters, software layers, and generic libraries. Using Tempo, our specializer for C, we show how partial evaluation can safely optimize implementations of those mechanisms. Because this optimization is automatic, it preserves the original genericity and extensibility of the implementation.     

Minimalist Z chair assembly by rotational dowel welding

The minimalist Z-chair of Rietveld is an example of a piece of furniture which is now possible to manufacture by using dowel welding technology.