Master equations for two qubits coupled via a nonlinear mode

A microscopic master equation describing the dynamics of two qubits coupled via a nonlinear mediator is constructed supposing that the two qubits, as well as the nonlinear mode, interact, each with its own independent bosonic bath. Generally speaking the master equation derived in this way represents a more appropriate tool for studying the dynamics of open quantum systems. Indeed we show that it is more complex than the phenomenological master equation, constructed simply adding ad hoc dissipative terms.     

The generation of hydrophilic polypeptide-siloxane conjugates via n-carboxyanhydride polymerisation

A novel methodology to create covalently linked polypeptide-siloxane hybrid materials by controlled n-carboxyanhydride ring opening polymerisation is disclosed. Poly-l-glutamic acid and poly-l-lysine conjugated products were formed that possessed excellent surface wettability. In addition, the poly-l-lysine-siloxane hybrids formed demonstrated bactericidal attributes against gram-positive Staphylococcus aureus and gram-negative Escherichia coli. It is anticipated that these materials may be of significance for the generation of hydrophilic siloxane-containing polymers that are commonly employed in contemporary medical devices.     

Microfluidic devices modulate tumor cell line susceptibility to NK cell recognition

This study aims to adoptively reduce the major histocompatibility complex class I (MHC-I) molecule surface expression of cancer cells by exposure to microfluid shear stress and a monoclonal antibody. A microfluidic system is developed and tumor cells are injected at different flow rates. The bottom surface of the microfluidic system is biofunctionalized with antibodies (W6/32) specific for the MHC-I molecules with a simple method based on microfluidic protocols. The antibodies promote binding between the bottom surface and the MHC-I molecules on the tumor cell membrane. The cells are injected at an optimized flow rate, then roll on the bottom surface and are subjected to shear stress. The stress is localized and enhanced on the part of the membrane where MHC-I proteins are expressed, since they stick to the antibodies of the system. The localized stress allows a stripping effect and consequent reduction of the MHC-I expression. It is shown that it is possible to specifically treat and recover eukaryotic cells without damaging the biological samples. MHC-I molecule expression on treated and control cell surfaces is measured on tumor and healthy cells. After the cell rolling treatment a clear reduction of MHC-I levels on the tumor cell membrane is observed, whereas no changes are observed on healthy cells (monocytes). The MHC-I reduction is investigated and the possibility that the developed system could induce a loss of these molecules from the tumor cell surface is addressed. The percentage of living tumor cells (viability) that remain after the treatment is measured. The changes induced by the microfluidic system are analyzed by fluorescence-activated cell sorting and confocal microscopy. Cytotoxicity tests show a relevant increased susceptibility of natural killer (NK) cells on microchip-treated tumor cells.     

Full-field optical coherence microscopy with a sub-nanosecond supercontinuum light source for material research

Full-field optical coherence microscopy performed with a supercontinuum light source based on a Q-switched micro-chip laser pumped photonic crystal fiber is shown to provide ultra-high resolution images from the interior of scattering samples. We exemplify our imaging approach by a detailed analysis of fiber reinforced polymer samples: comparative analysis by X-ray computed tomography as well as advanced data processing of the obtained 3D data is used to retrieve information on physical parameters like fiber orientation and distribution of point defect structures.     

A New Presenilin 1 (Psen1) Mutation (p.Cys263Trp) as a Cause of Both Early and Late-Onset Alzheimer’s Disease in a Large Italian Family

Mutations in the PSEN1 gene are the most common cause of autosomal dominant Alzheimer’s disease, and are characterized by a high phenotype variability. This study describes a five-generation family, with a prevalent late-onset of the disease and a high frequency of depression, in which a new missense mutation (c.789T > G, p.Cys263Trp) in exon 8 of the PSEN1 gene was found. Only the proband presented an early onset at the age of 45 with attention deficit, followed by spatial disorientation, psychiatric symptoms and parkinsonian signs. The other two cases had a late onset of the disease and a typical presentation with memory loss. Both were characterized by a high level of anxiety and depression. The disease course was different with signs of Lewy body dementia for the proband’s mother, and pyramidal involvement and a shorter disease duration for the proband’s maternal aunt. The other eight cases with late-onset dementia and three cases with a long history of depression have been reported in the family pedigree, underlying the high phenotype variability of PSEN1 mutations.     

Dansyl,a fluorescent photoprotecting group for microarray applications

BACKGROUND: The fluorescence dye 5‐dimethylamino‐1‐naphthalenesulfonyl chloride (Dansyl chloride) is commonly used for labeling the N‐terminus of proteins and peptides. Apart from the fluorescence, the ? SO₂? NH? bonds formed are susceptible to photolytic cleavage and will subsequently restore free amines. Consequently, Dansyl amides could act as a fluorescent photoprotecting group with novel application in solid phase synthesis or in microarray technologies. RESULTS: Commercial microscope glass slides were silanized with (3‐aminopropyl) triethoxysilane, exposed amines were activated with 1,4‐phenylene diisothiocyanate and subsequently reacted with dansylated polyethylene imine (PEI). The resulting fluorescence of the surface was determined and used as a measure of the homogeneity of the introduced functional groups. Using a mask, Dansyl‐PEI modified slides were locally exposed to photolytic cleavage within irradiation energy of 100 J cm^?2. Inscribed structures would be easily recognized due to their loss of fluorescence. The restored amines in deprotected areas were reacted with phosphorylated capture oligonucleotides followed by hybridization with complementary Cy5‐labeled targets. CONCLUSIONS: Capture probes immobilized precisely in structures exposed to UV‐light while non‐irradiated areas remained blocked. Such pre‐structured surfaces allow the production of highly reproducible microarrays without any specific problems of spotting imperfections. Gridding and segmentation of the determined sample allocation facilitate spot finding and spot analysis. Copyright © 2012 Society of Chemical Industry     

Post-transplant proteinuria associated with everolimus: Definition of main features with proteomics

Little is known on both the composition and mechanism(s) of proteinuria associated with the use of mTOR inhibitors, in particular of Everolimus (E). We characterized urinary proteins utilizing an integrated proteomics approach (quantitative essays, 2‐DE, MALDI‐TOF, Western blot) in 48 renal transplant recipients who were alternatively treated with E (n = 31) or with enteric coated mycophenolic acid (EC‐MPA) (n = 17). Twelve E patients (39%) developed high (>3 g/day) or intermediate proteinuria (1–3 g) compared to four (23%) of the EC‐MPA group. Urinary proteins (p<0.001), β2 microglobulin (p<0.001) and α1microglobulin (p<0.025) were higher in E than in EC‐MPA, appeared more rapidly and were inversely correlated with the day of treatment. Proteomics showed a marked increase of all urinary components in E and EC‐MPA patients, major changes involving typical components of glomerular damage (albumin, α1‐Zn glycoprotein, α2HS glycoprotein, leucin‐richα2‐glycoprotein) and specific bio‐markers for E (clusters of α1‐antitrypsin fragments and monoclonal λ chains). Finally, inter‐α‐trypsin‐inhibitor heavy chain H4 precursor was decreased in E and EC‐MPA urine compared to normal urine. In conclusion, E induced massive and generalized proteinuria of mixed glomerular and tubular origin that was correlated with the start of treatment and reached a nephrotic range in few cases. Specific urinary markers reflect renal alterations related to the transplant or specific alterations associated with the drug.     

Proteins and protein fragments in nephrotic syndrome: Clusters, specificity and mechanisms

Proteinuria is the hallmark of renal diseases and the characterization of the urinary protein composition may become an important source of information for diagnosis and research. So far, protein analysis in urine has been utilized for a generic individuation of site-specific defects (glomerular vs. tubular) but there is a need for an extension of proteomics to specific urinary biomarkers in selected clinical conditions. The identification of fragments of proteins in plasma and urine may increase the spectrum of urinary biomarkers. The unique speculative application so far proposed for protein fragments is nephrotic syndrome, and specifically focal segmental glomerulosclerosis, in which case they reflect intrinsic proteolysis occurring in plasma and represent surrogate biomarkers of the disease activity. Albumin is probably the most studied protein. Several of the albumin fragments present a peculiar distribution of the fingerprint peptide pattern containing both the N-terminal region and the C-terminal domain with a complete lack of any MS signals for the internal sequence region. Their characterization utilizing new strategies based on 2-D nondenaturing electrophoresis is now in progress. Studies on a direct characterization of proteases in plasma and urine will also define the participation of proteases to the genesis of renal diseases.     

Total water vapor column retrieval from MSG-SEVIRI split window measurements exploiting the daily cycle of land surface temperatures

A physically based algorithm for the retrieval of total water vapor column (TWC) over cloud-free land surfaces proposed by Kleespies and McMillin [Kleespies, J.T., McMillin L.M. (1990). Retrieval of precipitable water from observations in the Split Window over varying surface temperatures. Journal of Applied Meteorology, 29, 851-862.] is evaluated and extended for use in atmospheric correction and surface irradiance calculation schemes. Thermal infrared split window channels at 10.8 and 12.0 μm of the MSG-SEVIRI (Meteosat Second Generation-Spinning Enhanced Visible and Infrared Imager) instrument are used. The proposed algorithm takes advantage of the improved measurement capabilities of the MSG-SEVIRI instrument with its 15 min temporal resolution and its radiometric accuracy of 0.25 K and 0.37 K in the 10.8 and 12.0 μm channels. The temporal resolution allows exploitation of the daily land surface temperature variation. There is no further need for explicit auxiliary information on air and land surface temperatures, which is difficult to obtain on an operational basis. Updated coefficients for the split window parameterization are derived based on simulations of ‘top-of-atmosphere’ SEVIRI brightness temperatures for the globally representative Thermodynamic Initial Guess Retrieval (TIGR3) set of radiosonde profiles. It turns out that the linear dependency on the transmission ratio in both split window channels as originally proposed by Kleespies & McMillin [Kleespies, J.T., McMillin L.M. (1990). Retrieval of precipitable water from observations in the Split Window over varying surface temperatures. Journal of Applied Meteorology, 29, 851-862.] has to be extended towards a non-linear approach in order to make it applicable to the full range of global atmospheric conditions. Sensitivity studies reveal that the parameterization relies on the availability of input brightness temperatures with a variation larger than approximately 5 K during the daily cycle. The new TWC algorithm was tested with MSG-SEVIRI data for European and African regions for the period March-August 2004 and compared with radiosonde data. The results show that the algorithm is capable of producing TWC values with a mean bias of − 0.2 mm and an RMSE of 6.8 mm. From the total amount of 2583 coincidences for all viewing zenith angles both for winter and summer conditions, 82% were within a ± 5 mm and 94% were within a ± 10 mm difference interval between MSG-based and radiosonde-based TWC. A second comparison to European GPS measurements for the same period from March to August 2004 reveals a bias of − 3.0 mm and an RMSE of 6.0 mm. This result is obtained for 11 UTC GPS measurements which proved to match best the MSG-TWC values. Comparing MSG-TWC to daily cloud-free mean GPS values shows a lower bias of − 2.56 mm and an increased RMSE of 6.6 mm. These findings support the usefulness of the new MSG-based algorithm for surface irradiance calculations and atmospheric correction purposes.     

The dihydroxyacetonephosphate pathway for biosynthesis of ether lipids in Leishmania mexicana promastigotes

Microsatellites can be highly unstable and show a high level of polymorphism between individuals. Here we present the analysis of the CAG trinucleotide repeat polymorphism at the SBMA locus in 57 phenotypically normal individuals rigorously assigned to the Spanish Basque population. Results are compared with 100 Spanish non-Basque individuals who were already analyzed by us (175 alleles). This is the first study undertaken in these populations for this marker. In addition, we compared our results with those published for other populations. Relative allele frequencies showed differences between the samples and no unimodal distribution. The expected heterozygosity in the Basque sample was slightly lower than in the non-Basque sample. Conformity with Hardy-Weinberg equilibrium was verified by three tests. When compared with published data, the predominant alleles appear to be the same in the various populations. There are more differences between Basques and other Caucasoid samples than between non-Basques and Caucasoid samples. Population relationships were also examined by dendrograms based on genetic distances. The results obtained showed some peculiarities in the Basque population. The high degree of similarity with other dendrograms based on different markers and the efficiency of this STR marker in differentiating closely related populations, support the potential usefulness of microsatellites as tools for human population studies.