One‐step synthesis of bis‐macromonomers of poly(1,3‐dioxolane) catalyzed by Maghnite‐H+

Telechelic poly(1,3‐dioxolane) (PDXL) bis‐macromonomers bearing methyl methacrylate end groups were prepared by cationic ring‐opening polymerization of 1,3‐dioxolane (DXL), in the presence of methacrylic anhydride, catalyzed by Maghnite‐H⁺ (Mag‐H⁺), in bulk and in solution. Maghnite is a montmorillonite sheet silicate clay, which exchanged with protons to produce Mag‐H⁺. The influence of the amount of Mag‐H⁺, monomer (DXL), and methacrylic anhydride on monomer conversion was studied. The polymerization yield and the molecular weight of α,ω‐bis‐unsaturated PDXLs prepared depend on the amount of Mag‐H⁺ used and the reaction time. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci, 2006     

R.f. plasma deposition from hexamethyldisiloxane-oxygen mixtures

Hexamethyldisiloxane (HMDSO) has been used as a precursor for the deposition of silicon dioxide films at low substrate temperature (25–400°C) by plasma enhanced chemical vapour deposition processing. Effects of the partial pressure of oxygen in the discharge on the deposition rate and the composition of the films are investigated. The deposition rate is found to decrease with increasing oxygen concentration in the HMDSO/O₂ mixture. The chemical composition of the formed films was characterized by X-ray photoelectron spectroscopy and infrared spectroscopy. Over 40% of the oxygen in the gas phase the carbon content of films deposited from HMDSO/O₂ mixtures is less than 5%.     

Involvement of connexin 43 in meiotic maturation of bovine oocytes

In ovarian follicles, cumulus cells provide the oocyte with small molecules that permit growth and control maturation. These nutrients reach the germinal cell through gap junction channels, which are present between the cumulus cells and the oocyte, and between the cumulus cells. In this study the involvement of intercellular communication mediated by gap junction channels on oocyte maturation of in vitro cultured bovine cumulus-oocyte complexes (COCs) was investigated. The stages of oocyte maturation were determined by Hoechst 33342 staining, which showed that 90% of COCs placed in the maturation medium for 24 h progress to the metaphase II stage. Bovine COC gap junction communication was disrupted initially using n-alkanols, which inhibit any passage through gap junctions. In the presence of 1-heptanol (3 mmol l(-1)) or octanol (3.0 mmol l(-1) and 0.3 mmol l(-1)), only 29% of the COCs reached metaphase II. Removal of the uncoupling agent was associated with restoration of oocyte maturation, indicating that treatment with n-alkanols was neither cytotoxic nor irreversible. Concentrations of connexin 43 (Cx43), the major gap junction protein expressed in the COCs, were decreased specifically using a recombinant adenovirus expressing the antisense Cx43 cDNA (Ad-asCx43). The efficacy of adenoviral infection was > 95% in cumulus cells evaluated after infection with recombinant adenoviruses expressing the green fluorescence protein. RT-PCR performed on total RNA isolated from Ad-asCx43-infected COCs showed that the rat Cx43 cDNA was transcribed. Western blot analysis revealed a three-fold decrease in Cx43 expression in COCs expressing the antisense RNA for Cx43. Injection of cumulus cells with Lucifer yellow demonstrated further that the resulting lower amount of Cx43 in infected COCs is associated with a two-fold decrease in the extent of coupling between cumulus cells. In addition, oocyte maturation was decreased by 50% in the infected COC cultures. These results indicate that Cx43-mediated communication between cumulus cells plays a crucial role in maturation of bovine oocytes.     

Investigation of Time–Frequency Features for GPR Landmine Discrimination

Ground-penetrating radar (GPR) is capable to detect plastic antipersonnel landmines as well as other subsurface targets. In order to reduce false alarms, an option of automatic landmine discrimination from neutral minelike targets would be very useful. This paper presents a possibility for such discrimination and analyzes it experimentally. The authors investigate time-frequency features of an ultrawideband (UWB) target response for the discrimination between buried landmines and other objects. The discrimination method includes the extraction of an early-time target impulse response, its time-frequency transformation, and the extraction of time-frequency features based on a singular value decomposition of the transformed image. In order to take into account the changes in the UWB target signals, the experimental conditions are completely controlled to focus on the behavior of the target's response with respect to its depth and the horizontal position of the GPR above it. The dependence of the features on the GPR bandwidth is analyzed as well. The Mahalanobis distance is used as a criterion for optimal discrimination. The obtained results define the best features and conditions when the landmine discrimination is successful. For comparison, the discriminant power of the proposed features has been tested on a dataset, acquired during a field campaign in Angola     

基于基频特征的情感语音识别研究*

探索在不同的情感状态下的基音特征变化规律.通过对含有生气、高兴、悲伤情感语音信号进行分析,总结了情感语音基频的变化规律,确定了用于情感识别的12维的基频的基本特征以及扩展特征,运用混合高斯模型进行情感识别,并作了识别实验,获得了较好的结果.     

Dielectric behaviour of UV-crosslinked sulfonated poly (ether ether ketone) with methyl cellulose (SPEEK-MC) as proton exchange membrane

Proton exchange membrane materials based on sulfonated poly ether ether ketone (SPEEK) with Methyl Cellulose (MC) are developed by solution cast technique and exposed to UV radiation with Bezoin Ethyl Ether (BEE) as photoinitiator. The addition of MC into SPEEK polymer enhances the conductivity up to 8.7 × 10^?3 Scm^?1 at 30 °C temperature and 80% relative humidity. This new crosslinked hybrid membrane shows good prospect for the use as proton exchange membrane in fuel cell.