Cytological Observations and Bulked-Segregant Analysis Coupled Global Genome Sequencing Reveal Two Genes Associated with Pollen Fertility in Tetraploid Rice

Neo-tetraploid rice with high fertility is a useful germplasm for polyploid rice breeding, which was developed from the crossing of different autotetraploid rice lines. However, little information is available on the molecular mechanism underlying the fertility of neo-tetraploid rice. Here, two contrasting populations of tetraploid rice, including one with high fertility (hereafter referred to as JG) and another with low fertility (hereafter referred to as JD), were generated by crossing Huaduo 3 (H3), a high fertility neo-tetraploid rice that was developed by crossing Jackson-4x with 96025-4x, and Huajingxian74-4x (T452), a low fertility autotetraploid rice parent. Cytological, global genome sequencing-based bulked-segregant (BSA-seq) and CRISPR/Cas9 technology were employed to study the genes associated with pollen fertility in neo-tetraploid rice. The embryo sacs of JG and JD lines were normal; however, pollen fertility was low in JD, which led to scarce fertilization and low seed setting. Cytological observations displayed low pollen fertility (25.1%) and approximately 31.3 and 27.2% chromosome lagging at metaphase I and II, and 28.8 and 24.8% chromosome straggling at anaphase I and II in JD, respectively. BSA-seq of F_2–3 generations and RNA-seq of F₄ generation detected a common fragment, i.e., 18,915,234–19,500,000, at chromosome 7, which was comprised of 78 genes associated with fertility. Among 78 genes, 9 genes had been known to be involved in meiosis and pollen development. Two mutants ny1 (LOC_Os07g32406) and ny2 (LOC_Os07g32040) were generated by CRISPR/Cas9 knockout in neo-tetraploid rice, and which exhibited low pollen fertility and abnormal chromosome behavior. Our study revealed that two unknown genes, LOC_Os07g32406 (NY1) and LOC_Os07g32040 (NY2) play an important role in pollen development of neo-tetraploid rice and provides a new perspective about the genetic mechanisms of fertility in polyploid rice.     

Novel Organometallic Aromatic Azo Polyesters Based on Ferrocene

Some ferrocene containing aromatic azo polyesters were prepared by condensing 1,1-ferrocenedicarbonyl chloride with different azo groups containing aromatic diols, which were synthesized by diazotization coupling reaction from aromatic diamines. The polymeric products were separated as two portions on the basis of their solubility and subsequently characterized by their elemental analysis and FTIR spectral studies. Thermal properties of the products were measured by differential scanning calorimetry and thermogravimetry and the activation energies of pyrolysis were calculated using Horowitz and Metzger's method. The molecular weights of the soluble portions were determined by gel permeation chromatography (GPC) and these portions were also characterized by their inherent viscosities and UV-Vis spectroscopic studies.     

An image composition algorithm for handling global visual effects

Image composition is widely used in television and film industry to create synthetic visual effects. It requires seamless integration of different parts of two or more images into a single image. Existing image composition techniques only change the local contents of the resulting image while in many cases local changes may also require some global effects as well. For example, if the image of sun from one image is transferred to another image, the global brightness pattern should also be transferred. Unfortunately existing techniques cannot handle global effects of local content manipulations. This paper describes a novel image composition technique which captures global effects associated with a specific local content from one image and incorporates in the second image. In our proposed technique, all images are transformed to the frequency domain. The composite image is created in frequency domain by mixing different frequencies from multiple images and then transformed back to the spatial domain. We have experimented the proposed technique to shift the image of sun along with its global brightness pattern, the global effects of rain and also for transferring global texture pattern from one image to the other. In most of the cases the results produced by our algorithm appear far close to real images than state of the art existing image composition techniques.     

Indoor positioning: “an image-based crowdsource machine learning approach”

Multimedia Tools and Applications - Various technologies have been utilized today for recognizing client or user in the indoor areas. These technologies incorporate RSSI, Bluetooth Low Energy...     

Quality assessment of 3D synthesized images based on structural and textural distortion

Depth image based rendering (DIBR) is a popular technique for rendering virtual 3D views in stereoscopic and autostereoscopic displays. The quality of DIBR-synthesized images may decrease due to various factors, e.g., imprecise depth maps, poor rendering techniques, inaccurate camera parameters. The quality of synthesized images is important as it directly affects the overall user experience. Therefore, the need arises for designing algorithms to estimate the quality of the DIBR-synthesized images. The existing 2D image quality assessment metrics are found to be insufficient for 3D view quality estimation because the 3D views not only contain color information but also make use of disparity to achieve the real depth sensation. In this paper, we present a new algorithm for evaluating the quality of DIBR generated images in the absence of the original references. The human visual system is sensitive to structural information; any deg radation in structure or edges affects the visual quality of the image and is easily noticeable for humans. In the proposed metric, we estimate the quality of the synthesized view by capturing the structural and textural distortion in the warped view. The structural and textural information from the input and the synthesized images is estimated and used to calculate the image quality. The performance of the proposed quality metric is evaluated on the IRCCyN IVC DIBR images dataset. Experimental evaluations show that the proposed metric outperforms the existing 2D and 3D image quality metrics by achieving a high correlation with the subjective ratings.

     

Delay and link utilization aware routing protocol for wireless multimedia sensor networks

Wireless Multimedia Sensor Networks (WMSNs) consist of networks of interconnected devices involved in retrieving multimedia content, such as, video, audio, acoustic, and scalar data, from the environment. The goal of these networks is optimized delivery of multimedia content based on quality of service (QoS) parameters, such as delay, jitter and distortion. In multimedia communications each packet has strict playout deadlines, thus late arriving packets and lost packets are treated equally. It is a challenging task to guarantee soft delay deadlines along with energy minimization, in resource constrained, high data rate WMSNs. Conventional layered approach does not provide optimal solution for guaranteeing soft delay deadlines due to the large amount of overhead involved at each layer. Cross layer approach is fast gaining popularity, due to its ability to exploit the interdependence between different layers, to guarantee QoS constraints like latency, distortion, reliability, throughput and error rate. The paper presents a channel utilization and delay aware routing (CUDAR) protocol for WMSNs. This protocol is based on a cross-layer approach, which provides soft end-to-end delay guarantees along with efficient utilization of resources. Extensive simulation analysis of CUDAR shows that it provides better delay guarantees than existing protocols and consequently reduces jitter and distortion in WMSN communication.     

Multiple responses analysis and modeling of Fenton process for treatment of high strength landfill leachate

Landfill leachate is one of the most recalcitrant wastes for biotreatment and can be considered a potential source of contamination to surface and groundwater ecosystems. In the present study, Fenton oxidation was employed for degradation of stabilized landfill leachate. Response surface methodology was applied to analyze, model and optimize the process parameters, i.e. pH and reaction time as well as the initial concentrations of hydrogen peroxide and ferrous ion. Analysis of variance showed that good coefficients of determination were obtained (R2 > 0.99), thus ensuring satisfactory agreement of the second-order regression model with the experimental data. The results indicated that, pH and its quadratic effects were the main factors influencing Fenton oxidation. Furthermore, antagonistic effects between pH and other variables were observed. The optimum H2O2 concentration, Fe(II) concentration, pH and reaction time were 0.033 mol/L, 0.011 mol/L, 3 and 145 min, respectively, with 58.3% COD, 79.0% color and 82.1% iron removals.     

Three-qubit Grover’s algorithm using superconducting quantum interference devices in cavity-QED

We present a scheme for the implementation of three-qubit Grover’s algorithm using four-level superconducting quantum interference devices (SQUIDs) coupled to a superconducting resonator. The scheme is based on resonant, off-resonant interaction of the cavity field with SQUIDs and application of classical microwave pulses. We show that adjustment of SQUID level spacings during the gate operations, adiabatic passage, and second-order detuning are not required that leads to faster implementation. We also show that the marked state can be searched with high fidelity even in the presence of unwanted off-resonant interactions, level decay, and cavity dissipation.     

Performance analysis of multimedia based web traffic with QoS constraints

During the recent years, there has been a tremendous growth in the development and deployment of multimedia based networked applications such as video streaming, IP telephony, interactive games, among others. These applications, in contrast to elastic applications such as email and data sharing, are delay and delay jitter sensitive but can tolerate certain level of packet loss. A vital element of end-to-end delay and delay jitter is the random queueing delays in network switches and routers. Analysis of robust mechanisms for buffer management at network routers needs to be carried out in order to reduce end-to-end delay for traffic generated by multimedia applications. In this context, a threshold based buffer management scheme for accommodating multiple class multimedia traffic in network routers has been analysed. This technique effectively controls the allocation of buffer to various traffic classes according to their delay constraints. The forms of the joint state probabilities, as well as basic performance measures such as blocking probabilities are analytically established at equilibrium. Typical numerical experiments are included to illustrate the credibility of the proposed mechanism in the context of different quality of service (QoS) grades for various network traffic classes. This model, therefore, can be used as a powerful tool to provide a required grade of service to a particular class of multimedia based web traffic in any heterogeneous network.