Trace element concentrations in skin of free-ranging bottlenose dolphins (Tursiops truncatus) from the southeast Atlantic coast

Concentrations of trace elements (Al, As, Ba, Be, Cd, Co, Cr, Cu, Fe, Li, Mn, Ni, Pb, Sb, Se, Sn, Tl, U, V, Zn) and total mercury (THg) were determined in skin samples collected from free-ranging bottlenose dolphin (Tursiops truncatus) populations. Dolphins were captured in the estuarine waters of Charleston (CHS), South Carolina (n=74) and the Indian River Lagoon (IRL), Florida (n=75) during 2003, 2004 and 2005. A subset of the skin tissue samples were used to determine methylmercury (MeHg) levels in CHS (n=17) and IRL (n=8) bottlenose dolphins. Distributions of trace element concentrations by age (adult vs. juvenile), gender (male vs. female) and study area (CHS vs. IRL) were examined. In general, higher elemental skin concentrations were found in CHS adult males than those of IRL adult males, except for THg and MeHg. For CHS dolphins, adult females showed significantly higher THg levels than juvenile females while higher Mn levels were found in juvenile females. For IRL dolphins, adult males showed significantly higher As concentrations than that in juvenile males and females while higher Co and V levels were found in juvenile males than adult males. Of all elements measured in this study, significantly higher levels of Fe, Se and Zn concentrations in skin tissue of both dolphin populations were similar to other studies reported previously. Percentage of MeHg/THg in skin tissue of CHS and IRL dolphin was about 72% and 73%, respectively. Dietary levels of trace elements may play an important role in contributing to concentration differences for As, Co, Mn, Sb, Se, THg and Tl between CHS and IRL dolphins. Total Hg concentrations were significantly correlated with the age of CHS dolphins, while an inverse relationship was detected for Cu, Mn, Pb, U and Zn. The only significant correlation found between trace element concentration and IRL dolphins' age was Mn. Geographic differences in several trace element concentrations (As, Co, Mn, Sb, Se, THg and Tl) in skin tissue may be potentially useful to discriminate between dolphin populations and is a possibility that warrants further investigation.     

Optical fiber long-period grating humidity sensor with poly(ethylene oxide)/cobalt chloride coating

A long-period fiber grating (LPFG) humidity sensor is reported utilizing poly(ethylene oxide)/cobalt chloride (PEO/CoCl2) as a hybrid hygrosensitive cladding coating. A thin overlay of the material is deposited on the LPFG and with exposure to different ambient humidity levels, its spectral properties are modified. The material parameters associated with the sensing mechanism may include those of refractive index, absorption, and morphological alterations of the overlaid material. Relative humidity variations in the range from 50% to 95% have been detected with a resolution better than 0.2%. The response time constant of the fiber sensor is of the order of a few hundred milliseconds.     

Effect of different iron compounds on wheat and gluten‐free breads

BACKGROUND: Iron fortification of bread often results in sub‐optimal quality of the final product due to undesirable changes in the physical characteristics and sensory properties of the bread. In this study both the form of iron (soluble, insoluble or encapsulated) and the type of bread (wheat or gluten‐free) were varied in order to investigate the effect of iron and gluten on the product characteristics. RESULTS: The effect of iron on the quality characteristics of the breads investigated depended on iron type, but not on iron solubility. Colour, crust firmness, specific volume, cell number and uniformity as well as aroma were the attributes that were mainly affected in iron‐enriched wheat bread. In some cases, specific volume was 30% lower than that of the control sample, while cell uniformity was significantly lower, as low as 50% of the control sample in some fortified samples. In gluten‐free breads, differences between unfortified and fortified samples included colour, crust firmness, cell number, ‘moisture’ odour, metallic taste and stickiness. In some cases, the sensory scores were better for fortified samples. CONCLUSIONS: Differences due to iron fortification were less pronounced in gluten‐free compared to wheat breads. The choice of the appropriate iron compound which will not cause adverse quality changes is still a challenge. Copyright © 2010 Society of Chemical Industry     

On optimal cell planning: Case study for a DCS 1800 system

Micro and pico cell planning strategies are adopted in personal communication systems (PCS) in order to increase their capacity. The usage of the upper UHF band in combination with greater bandwidth is already proposed by telecom engineers in order to achieve the promised service quality and data rates. These strategies are characterized by an increased number of cells in specific geographical areas with the corresponding operating base transceiving stations (BTS) located at relatively low heights above the street level. In this case, the cell planning procedure in linear streets under line‐of‐sight (LOS) conditions needs further study concerning the technical characteristics of the PCS. In this paper, the propagation characteristics of a DCS 1800 system are investigated on a theoretical and experimental basis in a specific geographical area (center of Patras City in Northern Pelloponesse). An improved RF propagation model is proposed in order to determine the propagation path losses occurring under certain multipath fading conditions. Hence an optimum determination of a system's cellular area can be achieved. Copyright © 2001 John Wiley & Sons, Ltd.     

On the use of the BLYP functional for the DFT calculation of graphite–hydrogen systems

This letter focuses on a possible pitfall that can occur in the DFT calculation of graphite–hydrogen interactions under tokamaks conditions. Calculations based on the BLYP functional, in fact, result in a stable –C₂H₃ group that other functionals (OLYP, XLYP, PBE and BP) do not confirm.     

Transformation and Normal Vector Calculation of Parametrically Defined Surfaces Based on Dual Vectors and Screw Theory: Application to Phong's Shading Model

This paper presents a new approach for the transformation and normal vector calculation algorithms of parametrically defined surfaces via dual vectors and line transformations. The surface is defined via dual points, the transformation is performed by rotations and translations based on screw theory while normal vector calculation is utilized for shading based on Phong's illumination model. The main benefit of this approach lies into the compactness of the surface's representation since geometrical characteristics, such as tangent vectors, that are necessary for shading algorithms, are included within its definition. An extensive comparison is performed between the proposed approach and the traditional homogeneous model, presenting the merits of our approach. Analytical and experimental determination of the computational cost via computer implementation of 3D surface transformation and shading is presented. Point‐based methods for the representation, transformation and shading of parametrically defined surfaces are compared to the introduced line‐based methods (dual quaternions and dual orthogonal matrices). It is shown that the simplified rendering procedure of 3D objects, is considerably faster using screw theory over the traditional point‐based structures.     

Inverse Opal Scaffolds with Gradations in Mineral Content for Spatial Control of Osteogenesis

The design and fabrication of inverse opal scaffolds with gradations in mineral content to achieve spatial control of osteogenesis are described. The gradient in mineral content is established via the diffusion‐limited transport of hydroxyapatite nanoparticles in a closely packed lattice of gelatin microbeads. The mineral‐graded scaffold has an array of uniform pores and interconnected windows to facilitate efficient transport of nutrients and metabolic wastes, ensuring high cell viability. The graded distribution of mineral content can provide biochemical and mechanical cues for spatially regulating the osteogenic differentiation of adipose‐derived stromal cells. This new class of scaffolds holds promise for engineering the interfaces between mineralized and unmineralized tissues.     

Design and Fabrication of a Hierarchically Structured Scaffold for Tendon‐to‐Bone Repair

A hierarchically structured scaffold is designed and fabricated for facilitating tendon‐to‐bone repair. The scaffold is composed of three regions with distinct functions: (i) an array of channels to guide the in‐growth of cells and aligned deposition of collagen fibers, as well as integration of the scaffold with the tendon side, (ii) a region with a gradient in mineral composition to facilitate stress transfer between tendon and bone, and (iii) a mineralized inverse opal region to promote the integration of the scaffold with the underlying bone. Cell culture experiments confirm that adipose‐derived stromal cells are able to infiltrate and proliferate through the entire thickness of the scaffold without compromised cell viability. The seeded stem cells exhibit directed differentiation into tenocytes and osteoblasts along the mineral gradient as a response to the gradient in Young's modulus. This novel scaffold holds great promise to promote the formation of a functional tendon‐to‐bone attachment by offering a structurally and compositionally appropriate microenvironment for healing.     

Design and fabrication of drug‐eluting polymeric thin films for applications in ophthalmology

To study the development, characterisation, and drug release of one‐ and two‐layered thin films based on organic polymers [poly(D,L‐lactide‐co ‐glycolide) lactide:glycolide (65:35), poly(D,L‐lactide‐co ‐glycolide) lactide:glycolide (75:25), and polycaprolactone] and dexamethasone. To examine their applicability for intraocular lenses (IOLs) and function in intraocular drug delivery systems. Four series of thin films, single and double‐layer, were prepared by the spin‐coating method on a silicon substrate. The films were studied using atomic force microscopy and spectroscopic ellipsometry. The release rate of dexamethasone was studied for a period of ten weeks. Series A and C demonstrated the formation of large dexamethasone aggregates. The monolayer films of series C and D formed pores, in agreement with previous findings. The spectroscopic ellipsometry study demonstrated that the samples were transparent. The drug release study demonstrated that dexamethasone was released during the first 6 weeks at a desirable rate. The films exhibited properties suitable for use in intraocular drug delivery systems. The single‐layer thin films demonstrated a sufficient encapsulation of dexamethasone and appropriate release of the therapeutic substance. Further studies are necessary to investigate the possibility of developing the films directly on the surface of the IOL.Inspec keywords: eye, ellipsometry, spin coating, biomedical materials, polymer films, encapsulation, atomic force microscopy, drug delivery systems, aggregation, drugs, monolayers, ophthalmic lenses, polymer blendsOther keywords: IOL, intraocular drug delivery systems, spin‐coating method, atomic force microscopy, dexamethasone aggregates, monolayer films, organic polymers, spectroscopic ellipsometry, drug release, drug‐eluting polymeric thin films, ophthalmology, one‐layered thin films, two‐layered thin films, poly(D,L‐lactide‐co‐glycolide), polycaprolactone, intraocular lenses, dexamethasone release rate, dexamethasone encapsulation, time 6.0 week, Si     

State-complexity hierarchies of uniform languages of alphabet-size length

We study the state complexity of certain simple languages. If A$A$ is an alphabet of k$k$ letters, then a k$k$-language   is a nonempty set of words of length k$k$, that is, a uniform language of length k$k$. We show that the minimal state complexity of a k$k$-language is k+2$k+2$, and the maximal, (k^k−1−1)/(k−1)+2^k+1$(k^{k-1}-1)/(k-1)+2^k+1$. We prove constructively that, for every i$i$ between the minimal and maximal bounds, there is a language of state complexity i$i$. We introduce a class of automata accepting sets of words that are permutations of A$A$; these languages define a complete hierarchy of complexities between k²−k+3$k^2-k+3$ and 2^k+1$2^k+1$. The languages of another class of automata, based on k$k$-ary trees, define a complete hierarchy of complexities between 2^k+1$2^k+1$ and (k^k−1−1)/(k−1)+2^k+1$(k^{k-1}-1)/(k-1)+2^k+1$. This provides new examples of uniform languages of maximal complexity.