Reproducible Enhancement of Fluorescence by Bimetal Mediated Surface Plasmon Coupled Emission for Highly Sensitive Quantitative Diagnosis of Double‐Stranded DNA

Plasmonic enhancement of fluorescence from SYBR Green I conjugated with a double‐stranded DNA (dsDNA) amplicon is demonstrated on polymerase chain reaction (PCR) products. Theoretical computation leads to use of the bimetallic (Au 2 nm–Ag 50 nm) surface plasmons due to larger local fields (higher quality factors) than monometallic (Ag or Au) ones at both dye excitation and emission wavelengths simultaneously, optimizing fluorescence enhancement with surface plasmon coupled emission (SPCE). Two kinds of reverse Kretschmann configurations are used, which favor, in signal‐to‐noise ratio, a fluorescence assay that uses optically dense buffer such as blood plasma. The fluorescence enhancement (12.9 fold at maximum) with remarkably high reproducibility (coefficient of variation (CV) < 1%) is experimentally demonstrated. This facilitates credible quantitation of enhanced fluorescence, however unlikely to obtain by localized surface plasmons. The plasmon‐induced optical gain of 46 dB due to SPCE‐active dye molecules is also estimated. The fluorescence enhancement technologies with PCR enables LOD of the dsDNA template concentration of ≈400 fg µL^?1 (CV < 1%), the lowest ever reported in DNA fluorescence assay to date. SPCE also reduces photobleaching significantly. These technologies can be extended for a highly reproducible and sufficiently sensitive fluorescence assay with small volumes of analytes in multiplexed diagnostics.     

Integratable non-clogging microconcentrator based on counter-flow principle for continuous enrichment of CaSki cells sample

This study addresses the need to reduce the risk of clogging when preparing samples for cell concentration, i.e., the CaSki Cell-lines (epidermoid cervical carcinoma cells). Aiming to develop a non-clogging microconcentrator, we proposed a new counter-flow concentration unit characterized by the directions of penetrating flows being at an obtuse angle to the main flow, due to employment of streamlined turbine blade-like micropillars. Based on the optimization results of the counter-flow unit profile, a fractal arrangement for the counter-flow concentration unit was developed. A counter-flow microconcentrator chip was then designed and fabricated, with both the processing layer and collecting layer arranged in terms of the honeycomb structure. Visualized experiments using CaSki cell samples on the microconcentrator chip demonstrated that no cell-clogging phenomena occurred during the test and that no cells were found in the final filtrate. The test results show an excellent concentration performance for the microconcentrator chip, while a concentrating ratio of >4 with the flow rate being below 1.0 ml/min. As only geometrical structure is employed in the passive device, the counter-flow microconcentrator can be easily integrated into advanced microfluidic systems. Owing to the merit of non-clogging and continuous processing ability, the counter-flow microconcentrator is not only suitable for the sample preparation within biomedical field, but also applicable in water-particle separation.     

M/M/1 retrial queue with working vacations

In this paper we introduce the new M/M/1 retrial queue with working vacations which is motivated by the performance analysis of a Media Access Control function in wireless systems. We give a condition for the stability of the model, which has an important impact on setting the retrial rate for such systems. We derive the closed form solution in equilibrium for the retrial M/M/1 queue with working vacations, and we also show that the conditional stochastic decomposition holds for this model as well.     

The musculoskeletal system complaints of office workers at a vehicle production factory

This study aimed to determine the prevalence of and factors contributing to musculoskeletal system complaints (MSC) in office workers of a vehicle production factory and absenteeism due to these complaints. A total of 86.2% (n = 333) of the workers completed the survey. Of the workers, 73 (21.9%) were women, and 260 (78.1%) were men, and the mean age was 36.9 ± 8.75 years. The majority of the personnel had more than one symptom with a prevalence of 80.8%. The most frequently observed discomfort was tension and pain in the back muscles (58.6%) and in the neck muscles (58.3%), followed by low back pain (41%) and shoulder pain (30.3%). Pain in the shoulders, neck, and the back and pain and numbness in the fingers were statistically significantly more prevalent in employees who spent more than 5 hours per day performing desk work. Furthermore, all of the complaints, excluding pain in the elbows and knees, were statistically significantly more common in the women. When each complaint and the effects of contributing risk factors were analyzed by logistic regression analysis, pain in the shoulders, neck, and back; pain that spread to the arms; and pain in the fingers were correlated with longer work hours and the female gender (p < 0.05). The number of employees who had used sick leave within the past year due to MSCs was 11.7%. In conclusion, duration of work hours, frequency of breaks, and gender were determined as the most important risk factors in computer users regarding MSCs. © 2011 Wiley Periodicals, Inc.     

Application of neural processing paradigm in visual landmark recognition and autonomous robot navigation

This article addresses the issue of visual landmark recognition in autonomous robot navigation along known routes, by intuitively exploiting the functions of the human visual system and its navigational ability. A feedforward–feedbackward architecture has been developed for recognising visual landmarks in real time. It integrates the theoretical concepts from the pre-attentive and attentive stages in the human visual system, the selective attention adaptive resonance theory neural network and its derivatives, and computational approaches towards object recognition in computer vision. The architecture mimics the pre-attentive and attentive stages in the context of object recognition, embedding neural network processing paradigm into a computational template-matching approach in computer vision. The real-time landmark recognition capability is achieved by mimicking the pre-attentive stage, where it models a selective attention mechanism for optimal computational resource allocation, focusing only on the regions of interest to address the computational restrictive nature of current computer processing power. Similarly, the recognition of visual landmarks in both clean and cluttered backgrounds is implemented in the attentive stage by developing a memory feedback modulation (MFM) mechanism that enables knowledge from the memory to interact and enhance the efficiency of earlier stages in the architecture. Furthermore, it also incorporates both top-down and bottom-up facilitatory and inhibition pathways between the memory and the earlier stages to enable the architecture to recognise a 2D landmark, which is partially occluded by adjacent features in the surroundings. The results show that the architecture is able to recognise objects in cluttered backgrounds using real-images in both indoor and outdoor scenes. Furthermore, the architecture application in autonomous robot navigation has been demonstrated through a number of real-time trials in both indoor and outdoor environments.     

Optimization of forging processes using Finite Element simulations

During the last decades, simulation software based on the Finite Element Method (FEM) has significantly contributed to the design of feasible forming processes. Coupling FEM to mathematical optimization algorithms offers a promising opportunity to design optimal metal forming processes rather than just feasible ones. In this paper Sequential Approximate Optimization (SAO) for optimizing forging processes is discussed. The algorithm incorporates time-consuming nonlinear FEM simulations. Three variants of the SAO algorithm—which differ by their sequential improvement strategies—have been investigated and compared to other optimization algorithms by application to two forging processes. The other algorithms taken into account are two iterative algorithms (BFGS and SCPIP) and a Metamodel Assisted Evolutionary Strategy (MAES). It is essential for sequential approximate optimization algorithms to implement an improvement strategy that uses as much information obtained during previous iterations as possible. If such a sequential improvement strategy is used, SAO provides a very efficient algorithm to optimize forging processes using time-consuming FEM simulations.     

Maskless writing of microfluidics: Rapid prototyping of 3D microfluidics using scratch on a polymer substrate

We present a new rapid prototyping method designed for simple fabrication of 3D microfluidics using a maskless direct writing technique on polymer substrates. The entire process is enabled by a commercial cutter plotter with 10 μm resolution precision and high speed. A CAD design of top and bottom microstructures is directly written on a polymer substrate using a cutter plotter after setting up the suitable force. The smallest channel width of 20 μm was obtained with the minimum force and 100 μm from the maximum. Also the written depth increased linearly with force from 30 to 130 μm. Several 3D microfluidic devices are demonstrated using a maskless writing technique. The entire fabrication process from CAD layout to a final 3D device can be completed in 30 min outside the clean room facilities.