Optimized Biocatalytic Synthesis of 2-Selenopyrimidine Nucleosides by Transglycosylation**

Selenium-modified nucleosides are powerful tools to study the structure and function of nucleic acids and their protein interactions. The widespread application of 2-selenopyrimidine nucleosides is currently limited by low yields in established synthetic routes. Herein, we describe the optimization of the synthesis of 2-Se-uridine and 2-Se-thymidine derivatives by thermostable nucleoside phosphorylases in transglycosylation reactions using natural uridine or thymidine as sugar donors. Reactions were performed at 60 or 80 °C and at pH 9 under hypoxic conditions to improve the solubility and stability of the 2-Se-nucleobases in aqueous media. To optimize the conversion, the reaction equilibria in analytical transglycosylation reactions were studied. The equilibrium constants of phosphorolysis of the 2-Se-pyrimidines were between 5 and 10, and therefore differ by an order of magnitude from the equilibrium constants of any other known case. Hence, the thermodynamic properties of the target nucleosides are inherently unfavorable, and this complicates their synthesis significantly. A tenfold excess of sugar donor was needed to achieve 40−48 % conversion to the target nucleoside. Scale-up of the optimized conditions provided four Se-containing nucleosides in 6–40 % isolated yield, which compares favorably to established chemical routes.     

45 t门座起重机顶升方案设计

针对国内45 t门座起重机大车行走机构平衡梁裂缝问题。分析平衡梁裂缝产生原因并提出相应的维修方案,并对维修关键技术中的顶升方案设计,采用Ansys软件对其强度进行了有限元分析,同时对维修前后的平衡梁进行分析并对比。将维修方案应用于实际工程,取得了较好效果。该顶升机构修复方案设计符合工程实际,对工程实践具有一定的指导意义。     

黔北桑木场背斜铅锌矿深部找矿实例分析*#br#

黔北桑木场背斜位于上扬子地块黔北隆起区毕节弧形褶皱带与赤水克拉通盆地的交汇部位，属于有利的铅锌成矿区带。区内铅锌矿的赋矿层位主要有震旦系灯影组、寒武系清虚洞组、高台组及石冷水组，矿体多产于背斜核部并受断层或裂隙控制。通过对地层岩性特征、地表矿化特征、控容矿构造特征、化探异常特征、物探异常特征等进行综合研究，选择在成矿有利地段进行了工程验证，为进一步开展桑木场背斜铅锌矿深部找矿工作提供了思路。     

基于现场监测与数值模拟的浅层采空区稳定性分析

隐患采空区是目前影响露天开采矿山安全生产的主要危害源之一。随着台阶开采的不断剥离,露天开采境界内各台阶与地下空区群的隔离层厚度越来越薄,随时有可能发生采空区顶板坍塌事故。考虑到露天矿采空区地质赋存条件和围岩稳固性等特征,以弓长岭露天铁矿浅层采空区为工程背景,运用现场监测和数值模拟相结合的手段综合分析了浅层采空区的稳定性。将液体静力水准地表沉降监测系统的监测数据与FLAC数值模拟结果对比,调整蠕变参数使得数值模拟的蠕变速率与现场监测结果一致,而后据此进行未来结果的预测。最终根据地表沉降数据确定的蠕变参数取值为A=1.0×10^-12、m=1.75、n=0.35。研究表明:静力水准测点地表最大沉降位移为-9.8 mm,蠕变计算结果顶板最大垂直位移约20.4 mm,应力最大值约25 MPa,综合分析显示该采空区较稳定。上述研究提供了一种基于采空区现场监测数据的数值模拟蠕变分析方法,可为类似矿山采空区稳定性分析提供借鉴。     

Characterizations of friction welding joint interface for AISI 316

Because of heat amount is different from peripheral to central of friction welding interface, which is leaded to vary the characterizations along that interface. Current study, respectively, focused on the effect of different friction pressure on micro-structural and mechanical properties of that friction welding joint interface. Presently, these friction pressures are 110, 130, 150 and 170 MPa while kept all other conditions constant. The effects of different friction pressure on welding interface characterization were investigated by EDX, SEM, tensile, compression, impact and hardness tests. The tensile tests carried out on the standardized test piece with diameter 6 mm and 8 mm, thus, compression tests were extracted from the positions of 0°, 45° 90° with test specimen of 4 mm diameter and 6.5 mm length at weld center. Whereas, the impact test pieces were picked up in two positions, the first one is symmetrical, which it obtained to the respect of the rotation axis and the interface, on the other hand, the second one is non-symmetrical with the axis of rotation and symmetrical to the interface, for making the notch head coincide with the center of the welded joint, The obtained results showed that with reducing of friction pressure will present lack of bonding increasing from peripheral toward the welding center, which will responsible on reducing of the mechanical properties such as tensile, compression and impact strength.     

Increasing Volumetric CO2 Uptake of Hypercrosslinked Polymers through Composite Formation

To overcome the drawbacks of solid microporous materials for CO₂ capture, this proof‐of‐concept study demonstrates a low‐cost and rapid method for producing composites consisting of hypercrosslinked polymers (HCP) with a polyethyleneimine (PEI) binder. The resulting materials capture CO₂ through physical and chemical absorption simultaneously. Compared with HCP, the composites exhibit higher CO₂ uptake, higher volumetric density, and improved tolerance to water which is attributed to the PEI binder.     

干熄焦锅炉炉管失效机理分析

通过对干熄焦锅炉炉管及腐蚀产物开展系统研究，提出炉管失效原因为氧化/硫腐蚀＋高温粉尘冲刷。长寿命炉管仅耐磨层发生了较为严重的氧化及硫腐蚀，而近基体层发生了轻微氧化及硫腐蚀，基体只发生了轻微氧化；短寿命炉管耐磨层、近基体层以及基体裂纹内均发生了较为严重的氧化及硫腐蚀，且存在珠光体球化、内表面产生全脱碳层等缺陷。推测短寿命炉管存在超温现象，而超温可加剧氧化及硫腐蚀反应。此外，短寿命炉管遭受了较为严重的高温粉尘冲刷，不仅可造成炉管减薄，还会导致炉管表面温度升高。因此，减少循环气体中粉尘量尤其是大颗粒，可有效减弱冲刷以及控制炉管表面温度，是提高炉管使用寿命的关键。