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Detection of Glutamic Acid in Oilseed Rape Leaves Using Near Infrared Spectroscopy and the Least Squares-Support Vector Machine 总被引:1,自引:0,他引:1
Yidan Bao Wenwen Kong Fei Liu Zhengjun Qiu Yong He 《International journal of molecular sciences》2012,13(11):14106-14114
Amino acids are quite important indices to indicate the growth status of oilseed rape under herbicide stress. Near infrared (NIR) spectroscopy combined with chemometrics was applied for fast determination of glutamic acid in oilseed rape leaves. The optimal spectral preprocessing method was obtained after comparing Savitzky-Golay smoothing, standard normal variate, multiplicative scatter correction, first and second derivatives, detrending and direct orthogonal signal correction. Linear and nonlinear calibration methods were developed, including partial least squares (PLS) and least squares-support vector machine (LS-SVM). The most effective wavelengths (EWs) were determined by the successive projections algorithm (SPA), and these wavelengths were used as the inputs of PLS and LS-SVM model. The best prediction results were achieved by SPA-LS-SVM (Raw) model with correlation coefficient r = 0.9943 and root mean squares error of prediction (RMSEP) = 0.0569 for prediction set. These results indicated that NIR spectroscopy combined with SPA-LS-SVM was feasible for the fast and effective detection of glutamic acid in oilseed rape leaves. The selected EWs could be used to develop spectral sensors, and the important and basic amino acid data were helpful to study the function mechanism of herbicide. 相似文献
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阐述连接颈在钢球热斜轧中的作用,分析轧辊凸棱和连接颈之间的关系。利用有限元分析软件ANSYS对钢球热斜轧轧辊精整段凸棱进行结构参数的优化分析。结果表明,随着轧辊凸棱宽度尺寸的变化,最大变形量和应力与其成反比例关系。随着轧辊凸棱高度的增大,最大变形量也增大,而应力先减小后增大。轧辊凸棱切角对凸棱的变形量和应力影响较小。通过分析轧辊凸棱几何参数对强度和刚度等的影响规律,为钢球热斜轧轧辊的凸棱设计提供理论指导。 相似文献
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Yidan Zhang Renbing Jia Jing Wang Xiaofang Xu Yuting Yao Shengfan Ge Xianqun Fan 《International journal of molecular sciences》2013,14(7):15092-15104
Uveal melanoma (UM) is the most common primary intraocular malignancy and the leading potentially fatal primary intraocular disease in adults. Melanoma antigen recognized by T-cells (MART-1) has been studied extensively as a clinically important diagnostic marker for melanoma, however, its biological function remains unclear. In the present study, the UM cell line SP6.5, which showed a high level of MART-1 expression, was subjected to small interfering RNA-mediated silencing of MART-1. Silencing of MART-1 expression increased the migration ability of SP6.5 cells and down-regulated the expression of the metastasis suppressor NM23. Our results suggest that MART-1 is a candidate target for the development of therapeutic strategies for UM and in particular for the suppression of metastasis associated with this malignancy. 相似文献
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以温度控制计为例,论述了工艺流程图中反复使用的图形,在AutoCAD绘图中可以创建为块,并添加属性。针对各种类似的图形,将这些图形合理的制作为块,可以节省大量时间、加快绘图速度、提高绘图的准确性。 相似文献
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A nondestructive method for the classification of orange samples according to their growing conditions and geographic areas was developed using Vis/Near infrared spectroscopy. The results showed that the NIR spectra of the samples were moderately clustered in the principle component space and pattern recognition wavelet transform (WT) combined artificial neural network (BP-ANN) provided satisfactory classification results. Additionally, a partial least square (PLS) method was constructed to predict the sugar content of certain oranges. It showed excellent predictions of the sugar content of oranges, with standard error of prediction (SEP) values of 0.290 and 0.301 for Shatangju and Huangyanbendizao, respectively. 相似文献
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