Dual Inhibition of Pyruvate Dehydrogenase Complex and Respiratory Chain Complex Induces Apoptosis by a Mitochondria-Targeted Fluorescent Organic Arsenical in vitro and in vivo

Based on the potential therapeutic value in targeting mitochondria and the fluorophore tracing ability, a fluorescent mitochondria-targeted organic arsenical PDT-PAO-F16 was fabricated, which not only visualized the cellular distribution, but also exerted anti-cancer activity in vitro and in vivo via targeting pyruvate dehydrogenase complex (PDHC) and respiratory chain complexes in mitochondria. In details, PDT-PAO-F16 mainly accumulated into mitochondria within hours and suppressed the activity of PDHC resulting in the inhibition of ATP synthesis and thermogenesis disorder. Moreover, the suppression of respiratory chain complex I and IV accelerated the mitochondrial dysfunction leading to caspase family-dependent apoptosis. In vivo, the acute promyelocytic leukemia was greatly alleviated in the PDT-PAO-F16 treated group in APL mice model. Our results demonstrated the organic arsenical precursor with fluorescence imaging and target-anticancer efficacy is a promising anticancer drug.     

基于压水试验数据的渗透系数应力敏感性研究

岩体渗透率与应力关系是进行岩体渗流应力耦合分析的基础。基于龙滩水电站边坡岩体渗流压水试验结果和边坡岩体岩性及物理力学性质，对岩体渗透系数和地应力进行了分析计算，给出了边坡岩体渗透率随应力变化的关系式，为进行边坡岩体渗流一应力耦合分析提供了可靠数据。     

Properties of gap-aggregate gradation asphalt mixture and permanent deformation

Aggregate of natural crush stone in hot mix asphalt is applied to gap gradation mixed with the AC-20 binder, in accordance with Marshall mix design. Laboratory tests were conducted, and the single-factor variance analysis (ANOVA) is employed to estimate the significance of the gradation mixture properties. Based on permanent deformation tests and analytical results, sieve of 2.36 mm may be omitted for nominal maximum aggregate size (NMAS) of 12.5 mm, and sieve of 4.75 mm may be omitted for NMAS of 19 mm, in the case of the shortage of aggregate.     

Collocation methods: More efficient procedures for applying collocation

In recent years, new and more effective procedures for applying collocation have been published. This article is devoted to present a revision of this subject and complement its developments. From the general theory two broad approaches are derived, which yield the direct and the indirect TH-collocation methods. The former approach had not been published before, and it is a dual of the indirect approach. In particular, second order differential equations of elliptic type are considered and several orthogonal collocation algorithms are developed for them. In TH-collocation, the approximations on the internal boundary and in the subdomain interiors are completely independent. This yields clear computational advantages that are illustrated through the construction of such algorithms. In the implementations presented, three dimensional problems are included. In passing, single-point-collocation methods that have been the subject of several recent publications are revised.     

库水位变化对边坡稳定性影响的有限元模拟

在假定坡体孔隙水水位为水平线且不考虑渗透作用影响的基础上，基于强度折减有限元法分析了水位上升及下降速度对边坡稳定性的影响。对比计算表明，在库水位骤降或陡升条件下，相同库水位对应的边坡安全系数基本上均小于库水位缓慢变化情况下的安全系数。故在实际工程中，无论是排水还是蓄水，都应尽量保持水位缓慢变化，以使边坡处于相对较安全的状态。     

软土中的基坑支护工程实例分析

武汉某地下车库基坑处于深厚淤泥中 ,采用排桩 +土层锚杆支护与放坡 +网喷分段支护方式 ,本文根据现场地质、地形条件 ,对本工程支护过程中支护桩与土体变形作了具体分析。     

地震采集中检波器自然频率的试验分析

为了达到提高分辨率的目的，对不同自然频率的检波器进行了多次试验。通过试验分析认为，检波器在自然频率方面有所差别，在单炮记录上表现为对低、高频干扰压制情况有所不同，但是资料通过处理后，几种检波器的频谱和能量均无明显的差别。高分辨率勘探不等于高频勘探，而是一种宽频带的勘探，所以选择自然频率低的检波器有利于高分辨率勘探。