Regulation of the NADH supply by nuoE deletion and pncB overexpression to enhance hydrogen production in Enterobacter aerogenes

In this study, seven mutants from E. aerogenes IAM1183 wildtype were constructed via different strategies including deletion of lactate dehydrogenase, disruption of NADH dehydrogenase gene nuoE, overexpression of pncB and a combination of both to regulate of the NADH supply to enhance hydrogen production. Compared with the parental strain, the hydrogen yields of the strains IAM1183-E, IAM1183-L and IAM1183-EL increased by 23.3, 81.7 and 97.9%, respectively. When the pncB gene was overexpressed, the hydrogen yield of IAM1183/P, IAM1183-E/P, IAM1183-L/P and IAM1183-EL/P increased by 39.0, 6.5, 5.9, and 5.1% compared with the respective original knockout strains. Among them, the total hydrogen yield of strain IAM1183-EL/P with highest production efficiency was 58% higher than IAM1183. Further metabolite analysis indicated that the knockout of nuoE and ldhA, combined with the overexpression of pncB, resulted in a redistribution of the metabolic fluxes in E. aerogenes, which led to an improvement of the hydrogen yield.     

Microstructure and mechanical properties of hot-pressed SiC nanofiber reinforced SiC composites

This study reports on the preparation and mechanical properties of a novel SiC_nf/SiC composite. The single crystal SiC nanofiber(SiC_nf) reinforced SiC ceramic matrix composites (CMC) were successfully fabricated by hot pressing the mixture of β-SiC powders, SiC_nf and Al–B–C powder. The effects of SiC_nf mass fraction as well as the hot-pressing temperature on the microstructure and mechanical properties of SiC_nf/SiC CMC were systematically investigated. The results demonstrated that the 15 wt% SiC_nf/SiC CMC obtained by hot pressing (HP) at 1850 °C with 30 MPa for 60 min possessed the maximum flexural strength and fracture toughness of 678.2 MPa and 8.33 MPa m^1/2, respectively. The nanofibers pull out, nanofibers bridging and cracks deflection were found by scanning electron microscopy, which are believed can strengthen and toughen the SiC_nf/SiC CMC via consuming plenty of the fracture energy. Besides, although the relative density of the prepared SiC_nf/SiC CMC further increased with the sintering temperature rose to 1900 °C, the further coarsend composites grains results in the deterioration of the mechanical properties for the obtained composites compared to 1850 °C.     

Molecular imprinting strategy for solvent molecules and its application for QCM-based VOC vapor sensing

Cross-linked polymers made of methyl methacrylate (MMA) as a monomer and divinylbenzene (DVB) as a cross-linking agent were prepared in the presence of toluene or p-xylene as a solvent. The cross-linked polymer prepared in toluene tended to sorb toluene vapor preferably, while the cross-linked polymer prepared in p-xylene sorbed p-xylene vapor preferably. The observed molecular recognition ability can be explained on the bases of an imprinting effect by solvent molecules. Molecularly imprinted polymer (MIP) powders were blended with PMMA, and the blended films were coated on a piezoelectric quartz crystal with a view to preparing QCM-based VOC sensors. The imprint effect was clearly observed, even in these blended films. The response of the sensor towards toluene or p-xylene vapor was reversible; however, the response time was slow due to the existence of the matrix polymer around the MIP particles.     

Effect of high temperature alkali cooking on the constituents,structure and thermal degradation of hemp fiber

Degumming of hemp fiber by high temperature alkali cooking was investigated, and the effect of temperature and dosage of alkali on the constituents, structure, and thermal degradation of hemp fiber was also discussed in this work. The morphology, structure, and thermal degradation of hemp fiber after high temperature cooking were investigated through SEM, FTIR, WAXD, and TG analysis. The results indicated that the high temperature cooking process was effective for removing hemicelluloses and lignin and could also improve the thermal stability of cellulose. Crystallinity index of the treated hemp fiber was increased, which was related to the cooking temperature and NaOH dosage. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008     

Preparation of metallic silver from Ag2S slurry by direct hydrogen reduction under hydrothermal conditions

Metallic silver has been prepared by direct hydrogen reduction of Ag₂S in basic slurry under hydrothermal conditions. The XRD pattern and SEM micrographs of the reaction product are given. Metallic silver was predominant in the reduction product obtained by using ZnO as additive and PdCl₂ as catalyst at 220 °C, pH 12.0 and 2–3 MPa of partial hydrogen pressure for 4 h.     

Stability analysis for discrete linear systems with state saturation by a saturation-dependent Lyapunov functional

This paper concerns the stability analysis problem of discrete linear systems with state saturation using a saturation-dependent Lyapunov functional.We introduce a free matrix characterized by the sum of the absolute value of each elements for each row less than 1,which makes the state with saturation constraint reside in a convex polyhedron.A saturation-dependent Lyapunov functional is then designed to obtain a sufficient condition for such systems to be globally asymptotically stable.Based on this stability criterion,the state feedback control law synthesis problem is also studied.The obtained results are formulated in terms of bilinear matrix inequalities that can be solved by the presented iterative linear matrix inequality algorithm.Two numerical examples are used to demonstrate the effectiveness of the proposed method.     

图像处理中的颜色传递算法

介绍了C#中颜色传递的思路及实现,解决了图像处理中彩色图像中的颜色迁移,将图像A中的颜色信息转移到图像B中,使生成的图像C既保存原图像A的颜色信息又具有图像B的形状信息。     

可拓学在CPC产品开发过程建模中的应用

提出将可拓学应用产品开发过程领域,建立协同产品商务的理论体系及其支撑技术总体构架,借助过程仿真支撑环境,开发一条企业对产品开发过程进行创新性设计和自适应设计的途径,并在此基础上,开发实现了一个基于Web的模型驱动企业产品开发过程快速设计、优化与管理原型系统,从而为企业进行产品开发过程的创新模型设计和自适应模型设计提供新的思想、方法和工具,有助于企业在加入WTO后快速适应动态多变、不可预测的全球性市场竞争环境,缩小与发达国家同行们的差距。     

Ultrahigh-performance planar β-Ga_2O_3 solar-blind Schottky photodiode detectors

The low dark current, high responsivity and high specific detectivity could be preferably achieved in detectors based on junctions, owing to the efficient constraint of carriers. Compared with the other junctions, planar Schottky junctions have simple structures and technological demands and are easy integrated. Herein, in this work, we prepared the β-Ga_2O_3 thin film by metalorganic chemical vapor deposition method to construct planar Ti/β-Ga_2O_3/Ni Schottky photodiode detectors with different onstate resistances. Fortunately, all the devices exhibit state-of-the-art performances, such as responsivity of 175–1372 A W~(-1),specific detectivity of 10~(14) Jones and external quantum efficiency of 85700%–671500%. In addition, the dependences of device performances on the on-state resistances indicate that the higher dark currents, photocurrents and photoresponsivities may well be obtained when on-state resistance is smaller, due to the less external power is used to overcome the impendence and condensance at the Ti/β-Ga_2O_3 and Ni/β-Ga_2O_3 interfaces, but contributing to higher electric current flow both in the dark and under illuminations.