基于蛋白质组学技术研究秦川牛肉宰后贮藏过程中肌红蛋白含量及其衍生物转化

本实验采用4D-非标记蛋白质组学技术研究秦川牛肉贮藏过程中（0～8 d）肌红蛋白含量及其衍生物的转化情况，阐释冷却秦川牛肉中肌红蛋白含量及其衍生物转化的分子机制。结果表明：贮藏过程中，肌红蛋白表达量在宰后0～4 d上升、4～8 d下降，利用非标记蛋白质组学技术鉴定出与肌红蛋白及其衍生物相关的差异蛋白14 种，具体包括代谢酶、氧化还原酶、过氧化物酶、伴侣蛋白4 类，这4 类蛋白的表达共同调控贮藏过程中肌红蛋白含量的变化及其3 种衍生物之间的转化，具体表现为贮藏过程中肌红蛋白表达量整体呈下降趋势，氧合肌红蛋白相对含量持续下降，脱氧肌红蛋白、高铁肌红蛋白相对含量逐渐增加，导致肉色发生褐变。本研究结果有利于理解秦川牛肉贮藏过程肉类变色的复杂生化变化机制。     

Ni-Foam Structured Ni-Phyllosilicate Ensemble as an Efficient Monolithic Catalyst for CO2 Methanation

This work proposed a new path to synthesize Ni-phyllosilicate through the reaction of nickel hydroxide and silica sol on the surface of Ni-foam to form the monolithic Ni-phyllosilicate/Ni-foam catalyst. Ni-phyllosilicate could reprint the morphology of nickel hydroxid and firmly anchor on the framework of Ni-foam, which obtained fine Ni particles of 2.8 nm after reduction in H₂ at 650 °C, resulting in high catalytic activity for CO₂ methanation. In addition, the Ni-phyllosilicate/Ni-foam catalyst showed high long-term stability in a 100 h-lifetime test owing to the combined effects of surface confinement of Ni-phyllosilicate, firm anchoring between Ni-phyllosilicate and Ni-foam, as well as the high heat transfer property of Ni-foam.

Graphical Abstract

     

YSZ/MoS2 self-lubricating coating fabricated by thermal spraying and hydrothermal reaction

Thermal sprayed ceramic coatings have extensively been used in components to protect them against friction and wear. However, the poor lubricating ability severely limits their application. Herein, yttria-stabilized zirconia (YSZ)/MoS₂ composite coatings were successfully fabricated on steel substrate with the combination of thermal spraying technology and hydrothermal reaction. Results show that the synthetic MoS₂ powders are composed of numbers of ultra-thin sheets (about 7 ~ 8?nm), and the sheet has obvious lamellar structure. After vacuum impregnation and hydrothermal reaction, numbers of MoS₂ powders, look like flowers, generate inside the plasma sprayed YSZ coating. Moreover, the growing point of the MoS₂ flower is the intrinsic micro-pores of YSZ coating. The friction and wear tests under high vacuum environment indicate that the composite coating has an extremely long lifetime (>?100,000 cycles) and possesses a low friction coefficient less than 0.1, which is lower by about 0.15 times than that of YSZ coating. Meanwhile, the composite shows an extremely low wear rate (2.30?×?10^?7 mm³ N^?1 m^?1) and causes slight wear damage to the counterpart. The excellent lubricant and wear-resistant ability are attributed to the formation of MoS₂ transfer films and the ultra-smooth of the worn surfaces of hybrid coatings.     

结构化编程实现Sniffer

随着各高校校园网络规模的不断扩大和各种应用的急剧增加,各种新的网络问题也在不断出现。Sniffer是网络管理员进行网络管理的有效工具,可以有效地对网络进行实时监控,及时发现网络中存在的各种问题。本文首先介绍了Sniffer的工作原理,其次给出了实现Sniffer功能的编程流程图,并用C语言给出了编程所需的关键代码。     

Analysis and improvement of fair certified e-mail delivery protocol

Recently, Nenadić et al. proposed a novel fair exchange protocol RSA-CEMD [A. Nenadić, N. Zhang, S. Barton. Fair certified e-mail delivery, Proceedings of the 9th ACM Symposium on Applied Computing (SAC 2004)-Computer Security Track, Nicosia, Cyprus, pp. 391–396, 2004] for certified e-mail delivery with an off-line and transparent trusted third party. The protocol provides non-repudiation of origin and non-repudiation of receipt security service to protect communicating parties from each other's false denials that the e-mail has been sent and received. In this paper, we show that Nenadić's protocol cannot achieve the claimed fairness. In the exchange protocol, the receiver can cheat the sender successfully by sending an invalid verifiable and recoverable encryption of signature (VRES) which can pass all the sender's verifications, as the VRES scheme proposed in [A. Nenadić, N. Zhang, S. Barton. Fair certified e-mail delivery, Proceedings of the 9th ACM Symposium on Applied Computing (SAC 2004)-Computer Security Track, Nicosia, Cyprus, pp. 391–396, 2004] is inherently unrecoverable in some situations. In other words, there is always that the receiver can get the sender's e-mail message while the sender cannot obtain receiver's receipt. Furthermore, we propose a revised version of certified e-mail delivery protocol that preserves strong fairness while remaining optimistic.     

基于PC的开放式数控系统及其在玻璃雕刻机中的应用

论述了基于PC的开放式数控系统,重点对NC嵌入PC型开放式数控系统应用平台和应用软件的编制进行了研究,解决了构建NC嵌入PC型开放式数控系统的关键问题,并介绍了一个基于PC DMC1000运动控制卡的开放式数控玻璃雕刻机系统的开发实例。     

无线传感器网络在温室环境监测中的应用研究

以温室环境监测为应用背景,分析温室环境监控的特点,给出温室环境监控的系统体系结构,并结合温室监控的具体应用提出节能的数据融合算法,仿真实验表明该算法能有效提高节点能量的利用率和延长网络生命周期。     

自密实包浆再生混凝土早期塑性开裂试验研究

为了研究包浆再生粗骨料对自密实再生混凝土早期塑性开裂性能的影响,通过正交试验得出混凝土相对最优的配比方案。在此基础上,控制其他因素不变,以包浆水泥强度等级为单一变量,采用平板约束法,进行混凝土早期塑性开裂试验。结果表明:再生粗骨料经过包浆后,混凝土抗压与抗折强度均得到一定的提高;再生混凝土单位面积总开裂面积约为普通混凝土的1.5倍,经过水泥包浆后,开裂面积有了不同程度的减少,与未包浆的相比,32.5R、42.5R、52.5R水泥包浆的混凝土开裂面积分别减少了11.1%、25.1%、13.1%,其中42.5R水泥的包浆效果最好。结合微观电镜试验发现,骨料包浆可加强混凝土的界面过渡区,提高其抗开裂能力。     

宁夏汉延渠灌区灌溉水利用系数测算分析与评价

农田灌溉水利用系数表示从水源引水被农作物吸收利用的过程,是反应灌区灌溉用水效率的一项指标。本文以汉延渠灌区为例,综合考虑宁夏的气候环境、土壤类型、灌溉制度及作物种植结构等因素,选取典型渠段和样点灌区,分别采用"连乘法"和"首尾法"计算宁夏汉延渠灌区2018—2019年的灌溉水利用系数,并对其进行对比分析评价,从而确定灌区适宜的计算方法。