Regulation of the NADH supply by nuoE deletion and pncB overexpression to enhance hydrogen production in Enterobacter aerogenes

In this study, seven mutants from E. aerogenes IAM1183 wildtype were constructed via different strategies including deletion of lactate dehydrogenase, disruption of NADH dehydrogenase gene nuoE, overexpression of pncB and a combination of both to regulate of the NADH supply to enhance hydrogen production. Compared with the parental strain, the hydrogen yields of the strains IAM1183-E, IAM1183-L and IAM1183-EL increased by 23.3, 81.7 and 97.9%, respectively. When the pncB gene was overexpressed, the hydrogen yield of IAM1183/P, IAM1183-E/P, IAM1183-L/P and IAM1183-EL/P increased by 39.0, 6.5, 5.9, and 5.1% compared with the respective original knockout strains. Among them, the total hydrogen yield of strain IAM1183-EL/P with highest production efficiency was 58% higher than IAM1183. Further metabolite analysis indicated that the knockout of nuoE and ldhA, combined with the overexpression of pncB, resulted in a redistribution of the metabolic fluxes in E. aerogenes, which led to an improvement of the hydrogen yield.     

利用静电学和测量数据对碳纳米管膜场致发射规律的定量分析

碳纳米管膜的场致发射电流密度仅由它表面的宏观电场决定，无论其表面形状是平的还是半球状的。对于理想的平行板电极系统，其表面电场强度均匀（UId），发射电流密度、总电流与发射面积成正比：对于半球一平面电极系统半球形的阴极存在一个宏观场增强因子ks，一个与两极距离和球半径之比（d/r）相关的函数，其表面的平均场强为ks U/d。对于d/r=0，ks=1，d≥r的情况，ks接近于常数。对于10〈d／r〈100的情况，存在一个经验的表达式：ks=1＋0．15d/s=0．005（d／r）^2。在引入ks后，不同作者给出的平面电极系统和半球一平面电极系统碳纳米管膜的场致发射电流I与宏观表面电场强度E的关系都可以近似用-经验公式描述：I=a（E-Eo）^b，a，b为常数。该经验公式可为稳定生产的CNT膜片应用产品设计提供方便。     

Analysis of three-dimensional proximity effect in electron-beam lithography

In most of the proximity effect correction schemes, a two-dimensional model of proximity effect is employed by ignoring or averaging the variation of exposure along the depth dimension in the resist. However, as the feature size continues to decrease, the relative variation becomes significant so that it may need to be taken into account in proximity effect correction. In this study, the three-dimensional (3-D) proximity effect is analyzed in detail through computer simulation as a first step toward developing a 3-D proximity effect correction scheme. Effects of the parameters such as beam energy, resist thickness, feature size, developing threshold, etc., on the 3-D spatial distribution of exposure in the resist, in particular, depth-dependent proximity effect, are considered in the analysis. Results from the extensive simulation are presented in this paper.     

Some remarks on the reactor network synthesis for gold cyanidation

Gold cyanidation in most of the plants occurs in a cascade of large tanks because this configuration offers the longest residence time and consequently the highest gold recovery. However, the long contact between the ore and the leaching solution can increases the reactants consumption by side reactions, and in some cases this conventional reactor configuration will not be the most suitable. In this paper, several configurations for a gold leaching circuit with five equal-sized reactors are investigated and the effect of different network configurations on the process performance addressed. It is showed that, for high gold content and low cyanide concentration the conventional in-series configuration is the most recommended; however, for high cyanide concentration and low gold content the in-parallel configuration is the best one. For intermediate values of gold content and cyanide concentration, hybrid configurations with parallel sections at the beginning of the circuit are the best choice. It is also showed that the optimal circuit configuration strongly depends on the gold price and cyanide cost, and for low values of cyanide price the conventional in-series configuration is the most recommended, while for high cyanide prices the hybrid and parallel configurations must be taken into account.     

Analysis on the spatial ecological distribution model of landscape plant community

Because the spatial pattern of plant communities in garden landscapes usually exhibits highly non-random distribution characteristics, the accuracy of the analysis results is not high. For this purpose, a spatial ecological distribution model of landscape landform plant communities is designed. Based on the remote sensing images of landscape plant communities, the study area was obtained. The grassland communities in this area were classified and calculated by geostatistics, and the semi variogram values of typical samples of different vegetation types were obtained. According to the calculation results, the spatial terrain factors of landscape plant community are extracted, and the annual NDVI value is taken as the ecological vitality index of landscape green vegetation, the NDVI level is divided, the spatial ecological distribution model of landscape plant community is constructed, and the evaluation index system is generated, so as to complete the spatial ecological distribution model analysis of landscape plant community. The results show that the method of this study accurately analyzes the area of coniferous and broad-leaved mixed forest, mountain meadow, road, construction land, water area land and slope grade, which is slightly different from the actual value, indicating that the method has certain reliability.     

Government ecological governance management based on heterogeneous multi-processors and dynamic image sampling

With the advent of the governance economy era, traditional government governance models face enormous challenges. Governance management is the source of power to enter the era of the knowledge economy. This is a profound change that governments have needed to implement innovative government governance models, methods of social organization, and management. Governments in many countries are now adopting the construction of e-government as a strategy and response to demonstrate their priority development competitive advantage over the international competition and the challenges of the knowledge economy that spreads around the world. Therefore, the establishment of a new model of government governance has great theoretical and practical significance for impulse and government management innovation. The Dynamic Image Classification Algorithm (DICA) system is introduced in the implementation of the image processing technology and global disaster detection proposed here, and of this dynamic image classification algorithm (DICA) of the government, sector to extract more effectively. DICA is the most important task, or it is a strong shadow, proper access to the characteristics of the disaster area, and a false impact on the impact of the disaster, overcoming false alarms. It creates patches of post-disaster aerial image data from pre-tailoring and pre-adjustment training and satellite imagery. DICA training, all patches to government-managed data modified without delay or image data extraction. Two types of proposed systems are high-speed operation and accuracy (96.6%) and detection of earth disasters.     

A new admissibility condition of discrete-time singular systems with time-varying delays

This paper gives a novel delay-dependent admissibility condition of discrete-time singular systems with time-varying delays. For convenience, the time-varying delay is assumed to be the sum of delay lower bound and the integral multiples of a constant delay. Specially, if the constant delay is of unit length, the delay is an interval-like time-varying delay. The proposed admissibility condition is presented and expressed in terms of linear matrix inequality (LMI) by Lyapunov approach. Generally, the uncertainty of time-varying delay would lead to conservatism. In this paper, this critical issue is tackled by accurately estimating the time-varying delay. Consequently, the proposed admissibility condition is less conservative than the existing results, which is demonstrated by a numerical example.     

BFO optimized RLS algorithm for power system harmonics estimation

In this paper a new estimation approach combining both Recursive Least Square (RLS) and Bacterial Foraging Optimization (BFO) is developed for accurate estimation of harmonics in distorted power system signals. The proposed RLS–BFO hybrid technique has been employed for estimating the fundamental as well as harmonic components present in power system voltage/current waveforms. The basic foraging strategy is made adaptive by using RLS that sequentially updates the unknown parameters of the signal. Simulation and experimental studies are included justifying the improvement in performance of this new estimation algorithm.     

Fault tolerant H ∞ control for a class of polynomial non-linear discrete-time systems

This paper studies the fault tolerant control (FTC) problem for a class of polynomial nonlinear discrete-time systems with guaranteed H _∞ performance in the presence of actuator faults. The concerned fault is considered as a multi-model of the typical aberration in actuators’ effectiveness. A quadratic-like polynomial Lyapunov function is presented for the H _∞ specification. The main contribution of this paper is that the effect of the nonlinear terms appear in FTC analysis is described as an index in order to transform the controller design into a semi-definite programming (SDP). A numerical example is given to verify the applicability of this new approach for the nonlinear FTC synthesis.