Regulation of the NADH supply by nuoE deletion and pncB overexpression to enhance hydrogen production in Enterobacter aerogenes

In this study, seven mutants from E. aerogenes IAM1183 wildtype were constructed via different strategies including deletion of lactate dehydrogenase, disruption of NADH dehydrogenase gene nuoE, overexpression of pncB and a combination of both to regulate of the NADH supply to enhance hydrogen production. Compared with the parental strain, the hydrogen yields of the strains IAM1183-E, IAM1183-L and IAM1183-EL increased by 23.3, 81.7 and 97.9%, respectively. When the pncB gene was overexpressed, the hydrogen yield of IAM1183/P, IAM1183-E/P, IAM1183-L/P and IAM1183-EL/P increased by 39.0, 6.5, 5.9, and 5.1% compared with the respective original knockout strains. Among them, the total hydrogen yield of strain IAM1183-EL/P with highest production efficiency was 58% higher than IAM1183. Further metabolite analysis indicated that the knockout of nuoE and ldhA, combined with the overexpression of pncB, resulted in a redistribution of the metabolic fluxes in E. aerogenes, which led to an improvement of the hydrogen yield.     

Tunable structure and intensive upconversion photoluminescence for Ho3+-Yb3+ codoped bismuth titanate composite synthesized by sol-gel-combustion (SGC) method

Ho³⁺ and Yb³⁺ codoped bismuth titanate (BTO) composite powders with infrared to visible upconversion luminescence (UCL) function were prepared by SGC method. The effects of Ho³⁺ and Yb³⁺ doping content on the structure and property were investigated for BTO: xHo, 0.2 Yb (x = 0, 0.02, 0.04, 0.06, 0.08, 0.1) and BTO: 0.02Ho, yYb (y = 0.1, 0.2, 0.3, 0.5, 0.7, 0.9) samples. All the samples include three bismuth titanate phases (Bi₄Ti₃O₁₂, Bi₂Ti₂O₇, and Bi₂₀TiO₃₂), and the phase proportion can be tuned by changing Ho³⁺ and Yb³⁺ doping content. These powders are well crystalized with honeycomb-like microscopic structure, and with good absorption for 233 nm, 310 nm and 975 nm wavelength. The band gap can be tuned from 3.53 eV to 4.03 eV when increasing Yb³⁺ content from y = 0 to y = 0.9. A strong 530–580 nm green emission band and a relative weak 630–690 nm red one corresponding to Ho³⁺: ⁵S₂ → ⁵I₈ and ⁵F₅ → ⁵I₈ transitions appear in the UCL spectra for all the BTO: Ho, Yb samples when pumped at 980 nm. The emission intensities can well be tuned with various Ho³⁺ and Yb³⁺ content. The optimal UCL was obtained in BTO: 0.02Ho, 0.5 Yb for all the prepared samples. The energy transfer mechanism is analyzed by building a two-photon energy transfer model, which is proved by the relationship between emission intensities and pumping power measurement. The concentration quenching of Ho³⁺ is caused by cross relaxation of CR₁ and CR₂ (Ho: ⁵F₄, ⁵S₂ + ⁵I₈ → ⁵I₄ + ⁵I₇) and by CR₃ (Ho: ⁵F₄, ⁵S₂ + Yb: ²F_7/2 → Ho: ⁵I₆ + Yb: ²F_5/2) for Yb³⁺ quenching. The mean luminescence lifetime (τ_m) from Ho: ⁵S₂ decreases monotonously with the increase of Ho³⁺ and Yb³⁺ content.     

A simple approach for fabrication of interconnected graphitized macroporous carbon foam with uniform mesopore walls by using hydrothermal method

Three-dimensional (3D) highly interconnected graphitized macroporous carbon foam with uniform mesopore walls has been successfully fabricated by a simple and efficient hydrothermal approach using resorcinol and formaldehyde as carbon precursors. The commercially available cheap polyurethane (PU) foam and Pluronic F127 were used as a sacrificial polymer and mesoporous structure-directing templates, respectively. The graphitic structure of carbon foam was obtained by catalytic graphitization method using iron as catalyst. Three different carbon foams such as graphitized macro-mesoporous carbon (GMMC) foam, amorphous macro-mesoporous carbon (AMMC) foam and graphitized macroporous carbon (GMC) foam were fabricated and their physicochemical and mechanical properties were systematically measured and compared. It was found that GMMC possess well interconnected macroporous structure with uniform mesopores located in the macroporous skeletal walls of continuous framework. Besides, GMMC foam possesses a well-defined graphitic framework with high surface area (445 m²/g), high pore volume (0.35 cm³/g), uniform mesopores (3.87 nm), high open porosity (90%), low density (0.30 g/cm³) with good mechanical strength (1.25 MPa) and high electrical conductivity (11 S/cm) which makes it a promising material for many potential applications.     

Some remarks on the reactor network synthesis for gold cyanidation

Gold cyanidation in most of the plants occurs in a cascade of large tanks because this configuration offers the longest residence time and consequently the highest gold recovery. However, the long contact between the ore and the leaching solution can increases the reactants consumption by side reactions, and in some cases this conventional reactor configuration will not be the most suitable. In this paper, several configurations for a gold leaching circuit with five equal-sized reactors are investigated and the effect of different network configurations on the process performance addressed. It is showed that, for high gold content and low cyanide concentration the conventional in-series configuration is the most recommended; however, for high cyanide concentration and low gold content the in-parallel configuration is the best one. For intermediate values of gold content and cyanide concentration, hybrid configurations with parallel sections at the beginning of the circuit are the best choice. It is also showed that the optimal circuit configuration strongly depends on the gold price and cyanide cost, and for low values of cyanide price the conventional in-series configuration is the most recommended, while for high cyanide prices the hybrid and parallel configurations must be taken into account.     

基于多维产出ZSG-DEA模型的中国水资源污染综合分配效率测算

利用考虑多维产出的零和收益-数据包络分析(ZSG-DEA)模型测算2000—2017年中国大陆31个省级行政区(以下简称省)在水资源污染排放总量固定下的污染综合分配效率及生产、生活与生态3个维度的产出效率,并基于效率最大化原则对2017年各省污染排放额度进行调整。结果表明:31个省污染综合分配效率的平均值由2000年的0.322上升至2017年的0.364,生产维度产出效率是拉动污染综合分配效率提升的主要动力;2000—2017年31个省污染综合分配效率的平均值为0.341,生态产出过量与生活维度产出不足导致污染综合分配效率处于较低水平;污染综合分配效率、生态维度产出效率与生活维度产出效率受经济发展水平影响较小,而生产维度产出效率受经济发展水平影响较大;污染综合分配效率分布由大到小为东部、西部、中部,生产维度产出效率分布由大到小为东部、中部、西部,生态维度产出效率分布由大到小为西部、中部、东部,生活维度产出效率分布由大到小为西部、东部、中部;各省污染排放调整额度与污染综合分配效率呈正相关关系,北京、天津、上海、浙江、青海与西藏等20个效率较高的省可增加污染排放额度,河北、安徽、河南、江西与湖北等11个效率较低的省需降低污染排放额度。     

Analysis on the spatial ecological distribution model of landscape plant community

Because the spatial pattern of plant communities in garden landscapes usually exhibits highly non-random distribution characteristics, the accuracy of the analysis results is not high. For this purpose, a spatial ecological distribution model of landscape landform plant communities is designed. Based on the remote sensing images of landscape plant communities, the study area was obtained. The grassland communities in this area were classified and calculated by geostatistics, and the semi variogram values of typical samples of different vegetation types were obtained. According to the calculation results, the spatial terrain factors of landscape plant community are extracted, and the annual NDVI value is taken as the ecological vitality index of landscape green vegetation, the NDVI level is divided, the spatial ecological distribution model of landscape plant community is constructed, and the evaluation index system is generated, so as to complete the spatial ecological distribution model analysis of landscape plant community. The results show that the method of this study accurately analyzes the area of coniferous and broad-leaved mixed forest, mountain meadow, road, construction land, water area land and slope grade, which is slightly different from the actual value, indicating that the method has certain reliability.     

Government ecological governance management based on heterogeneous multi-processors and dynamic image sampling

With the advent of the governance economy era, traditional government governance models face enormous challenges. Governance management is the source of power to enter the era of the knowledge economy. This is a profound change that governments have needed to implement innovative government governance models, methods of social organization, and management. Governments in many countries are now adopting the construction of e-government as a strategy and response to demonstrate their priority development competitive advantage over the international competition and the challenges of the knowledge economy that spreads around the world. Therefore, the establishment of a new model of government governance has great theoretical and practical significance for impulse and government management innovation. The Dynamic Image Classification Algorithm (DICA) system is introduced in the implementation of the image processing technology and global disaster detection proposed here, and of this dynamic image classification algorithm (DICA) of the government, sector to extract more effectively. DICA is the most important task, or it is a strong shadow, proper access to the characteristics of the disaster area, and a false impact on the impact of the disaster, overcoming false alarms. It creates patches of post-disaster aerial image data from pre-tailoring and pre-adjustment training and satellite imagery. DICA training, all patches to government-managed data modified without delay or image data extraction. Two types of proposed systems are high-speed operation and accuracy (96.6%) and detection of earth disasters.     

Fault tolerant H ∞ control for a class of polynomial non-linear discrete-time systems

This paper studies the fault tolerant control (FTC) problem for a class of polynomial nonlinear discrete-time systems with guaranteed H _∞ performance in the presence of actuator faults. The concerned fault is considered as a multi-model of the typical aberration in actuators’ effectiveness. A quadratic-like polynomial Lyapunov function is presented for the H _∞ specification. The main contribution of this paper is that the effect of the nonlinear terms appear in FTC analysis is described as an index in order to transform the controller design into a semi-definite programming (SDP). A numerical example is given to verify the applicability of this new approach for the nonlinear FTC synthesis.     

一种视频驱动的三维虚拟人动画实现方法

研究实现三维人体动画具有广泛的应用前景和实用意义,提出了一种二维视频驱动的三维人体动画实现方法。基于动态帧的关键帧提取算法从二维视频中构建了二维关键帧集合;基于二维关键帧构建二维人体骨骼模型;利用小孔成像原理和勾股定理计算得到关节特征点的深度坐标,从而得到了反映人体动画的三维数据。实验结果表明,该方法生成的三维人体动画形象逼真、成本低、提高了运动生成的实时性,能够应用于虚拟现实、计算机游戏、三维视频游戏制作等领域。     

Preparation and Characterization of K_n|Ga(H_2O)MW_(11)O_(39)|·xH_2O

In this paper the heteropoly complexes K_n[Ga(H_20)MW_(11)O_39)]·xH_20 [M=Cu(Ⅱ), Co(Ⅱ), Cr(Ⅲ),Fe(Ⅲ), Mn(Ⅳ)] have been prepared in this laboratory firstly. The products were characterized by chemicalanalysis, ionexchange, IR, UV and X-ray powder diffraction.