The nuclear actin-related protein Act3p/Arp4p is involved in the dynamics of chromatin-modulating complexes

Chromatin remodelling and histone-modifying complexes govern the modulation of chromatin structure. While components of these complexes are diverse, nuclear actin-related proteins (Arps) have been repeatedly found in these complexes from yeast to mammals. In most cases, Arps are required for functioning of the complexes, but the molecular mechanisms of nuclear Arps have as yet been largely unknown. The Arps and actin, sharing a common ancestor, are supposed to be highly similar in the three-dimensional structure of their core regions, including the ATP-binding pocket. The Arp Act3p/Arp4p of Saccharomyces cerevisiae exists within the nucleus, partly as a component of several high molecular mass complexes, including the NuA4 histone acetyltransferase (HAT) complex, and partly as uncomplexed molecules. We observed that mutations in the putative ATP-binding pocket of Act3p/Arp4p increased its concentration in the high molecular mass complexes and, conversely, that an excess of ATP or ATPgammaS led to the release of wild-type Act3p/Arp4p from the complexes. These results suggest a requirement of ATP binding by Act3p/Arp4p for its dissociation from the complexes. In accordance, a mutation in the putative ATP binding site of Act3p/Arp4p inhibited the conversion of the NuA4 complex into the smaller piccoloNuA4, which does not contain Act3p/Arp4p and exhibits HAT activity distinct from that of NuA4. Although the in vitro binding activity of ATP by recombinant Act3p/Arp4p was found to be rather weak, our observations, taken together, suggest that the ATP-binding pocket of Act3p/Arp4p is involved in the function of chromatin modulating complexes by regulating their dynamics.     

HT－6M托卡马克电源暂态过程分析

采用ＥＭＴＰ（ＥｌｅｃｔｒｏＭａｇｎｅｔｉｃＴｒａｎｓｉｅｎｔＰｒｏｇｒａｍ）程序对ＨＴ－６Ｍ（合肥托卡马克装置）电源进行了暂态过程的计算分析。找出了电源暂态过程与托卡马克装置放电波形之间的联系。为今后的电源设计、故障分析提供了更多的依据。     

ATP-regulation of antioxidant properties and phenolics in litchi fruit during browning and pathogen infection process

The impact of energy level on antioxidant properties in relation to pericarp browning and loss of disease resistance of litchi fruit was investigated. Litchi fruits were vacuum-infiltrated with distiled water (control), 1 mM adenosine triphosphate (ATP) and 0.5 mM 2,4-dinitrophenol (DNP) under 75 kPa for 3 min before being inoculated with Peronophythora litchi or not. ATP-treated fruits presented higher activities of antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX). Higher activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, reducing power and contents of phenolic compounds were also observed in ATP-treated fruit during storage. In contrast, DNP treatment lowered the enzymes activities, scavenging ability and the contents of phenolic compounds. Higher levels of antioxidant enzymatic system and non-enzymatic system were observed in P. litchii-inoculated fruits than those in non-inoculated fruits. Application of ATP and DNP exhibited a similar change patterns and effects in inoculated fruits. When related to previously reported ATP levels, the results suggested that ATP levels could regulate the antioxidant system. Sufficient available energy is crucial for inhibiting browning and preventing the loss of disease resistance in harvested litchi fruit.     

线路遭受连续雷击造成断路器事故分析

介绍一起由于线路遭受连续雷击造成线路断路器发生断口击穿的事故,基于雷电波传播理论,分析了致使断路器断口击穿的原因,又根据现场实际情况,建立一个仿真模型,利用过电压计算软件EMTP(Electromagnetic Transient Program)对该模型进行仿真分析,理论分析和模拟仿真证明,如果断路器断口很容易被雷电过电压击穿,针对此类情况,提出在断路器进(出)线侧安装金属氧化锌避雷器,如变电站内无安装位置,可在线路终杆塔上安装带间隙的氧化锌避雷器。     

10kV配电网系统弧光接地过电压研究

比较了中性点直接接地、不接地、经消弧线圈接地等接地方式的安全性和供电特点,从理论上利用EMTP/ATP电磁暂态程序建立仿真模型,以吉林省66 kV某变电站发生的10 kV单相接地故障为例,计算了弧光接地过电压并分析了降低弧光接地过电压的措施,得出中性点经消弧线圈接地,能够降低弧光接地过电压的幅值;接地残流小于10 A时接地电弧易于自熄弧;弧光接地过电压标幺值由中性点不接地时的2.01降至0.97;在脱谐度绝对值相等条件下,消弧线圈工作在过补偿和欠补偿状态下的弧光接地过电压幅值相差不大。     

基于PID神经元网络控制的空间光通信ATP跟踪性能分析

针对空间光通信中跟踪系统的高精度、宽带宽要求,提出了一种基于PID神经元网络(Proportional integral differential neural network-PIDNN)的控制方案.采用MATLAB对所建立的跟踪系统模型进行了仿真分析研究,对采用PIDNN控制器的精跟踪系统的在线训练能力及学习、调整功能进行了仿真验证,同时加入扰动源对精跟踪系统的稳态、动态性能及鲁棒性进行了仿真测试.仿真和测试结果表明:通过PIDNN控制的精跟踪系统具有良好的稳态及动态性能和很强的鲁棒性,系统跟踪精度高且系统带宽较宽.     

大尺寸弱阴离子交换晶胶层析一步法分离ATP的实验研究

使用一种新型的大尺寸弱阴离子交换晶胶柱从酵母细胞发酵液中一步分离纯化广泛应用于临床治疗的高能化合物三磷酸腺苷(ATP)。该晶胶柱高150mm,直径140mm,柱体体积达到2300mL。实验测定了晶胶柱的孔隙率和等板高度等基本性能参数。层析过程以去离子水为冲洗液,分别用0.03、0.4和1mol·L-1NaCl溶液在2cm·min-1流速下进行洗脱。发现0.03mol·L-1NaCl溶液能把大部分杂质洗脱,0.4mol·L-1NaCl溶液洗脱后能获得纯度较高的ATP。洗脱下来的ATP样品通过高效液相色谱(HPLC)定量分析,回收的ATP纯度达95%～97%,总回收率49.1%。与其它已有方法相比,本法耗时少,工艺简单,降低了ATP变性的风险;在层析过程中以去离子水代替酸液作为冲洗剂和洗脱液溶剂,降低分离过程成本。     

基于遗传算法的高压长线路双端故障测距研究

为提高高压长线路故障定位的准确度,提出了一种基于遗传算法的故障定位新方法。该方法基于线路分布参数模型,按照故障线路两端沿线向对端推得的故障点电压幅值相等的原则列写故障测距方程,并通过相量法对电压分布函数的单调性进行分析,得到伪根判别法则的数学表达式;引入遗传算法求解故障测距方程,采用群体搜索和多父体重组策略,自适应和容错能力强且能够保证解群搜索的遍历性和快速性;在一次搜索中算法可求得故障距离的多个解,有利于伪根的判别。ATP仿真结果和实际应用证明了该方法不受过渡阻抗、故障类型及不同步角的影响,计算速度快且精确度高。     

500kV HGIS变电站雷电侵入波的计算分析

针对500 kV HGIS新型设备不断涌现,以往建立的变电站雷电侵入波计算模型需要改进的问题,采用HGIS新型设备的某500kV变电站,分析设备生产厂家提供的HGIS结构参数和试验参数,建立其雷电过电压计算模型,并结合以往采用常规设备变电站的设计经验,优化变电站雷电过电压计算方法,采用国际通用的EMTP电磁暂态仿真计算程序,对500kV HGIS变电站的雷电侵入波作了较为深入的计算分析和研究,提出了该工程针对雷电过电压500kV母线侧可不加装避雷器,出线侧需加装避雷器的配置方案,该研究可供变电站雷电过电压建模仿真计算参考。     

电力变压器励磁涌流和故障电流仿真研究

在研究电力变压器励磁涌流和故障电流特性的基础上 ,应用电磁暂态程序 (EMTP) ,采用不同的模型和方法对变压器励磁涌流和故障电流进行了全面系统的仿真计算和分析。结果表明 ,EMTP能够根据不同的条件和要求有效地实现对变压器励磁涌流和故障电流的仿真