Cyclic variations in the permeability of the cell wall of Saccharomyces cerevisiae.

To study cell-cycle-related variations in wall permeability of Saccharomyces cerevisiae, two approaches were used. First, an asynchronous culture was fractionated by centrifugal elutriation into subpopulations containing cells of increasing size. The subpopulations represented different stages of the cell cycle as judged by light microscopy. Cell wall porosity increased when these subpopulations became enriched with budded cells. Secondly, synchronous cultures were obtained by releasing MATa cells from alpha-factor induced G1-arrest. These cultures grew synchronously for at least two generations. The cell wall porosity increased sharply in these cultures, shortly before buds became visible and was maximal during the initial stages of bud growth. It decreased in cells which had completed nuclear migration and before abscission of the bud had occurred. The porosity reached its lowest value during abscission and in unbudded cells. We examined the incorporation of mannoproteins into the wall during the cell cycle. SDS-extractable mannoproteins were incorporated continuously. However, the incorporation of glucanase-extractable mannoproteins, which are known to affect cell wall porosity, showed cyclic oscillations and reached its maximum after nuclear migration. This coincided with a rapid decrease in cell wall porosity, indicating that glucanase-extractable mannoproteins might contribute to this decrease.     

单片机在监控同步电动机运行功率因数中的应用

介绍如何利用单片机自动控制同步电动机运行时的功率因数。     

液压同步剪系统的设计

本文通过对蜂窝陶瓷载体成型过程的分析，指出在其成型过程中存在的问题，提出了采用液压同步剪解决这些问题的方法。论文分析了同步剪的工作原理，给出了其液压原理图及其设计特点。     

螺旋管簧的可靠性优化设计

讨论了螺旋管簧的可靠性优化设计问题。在基本随机变量的概率特性已知的情况下，采用二阶矩法和可靠性优化设计方法对螺旋管簧进行了可靠性优化设计。通过计算机程序可以直接实现螺旋管簧的可靠性优化设计，迅速准确地得到螺旋管簧的可靠性优化设计信息。     

大型结构物自动称重过程的计算机控制技术

大型结构物的称重通过多个千斤顶同步顶升实现。大型结构物往往存在柔度大、重量分布不均、支点跨距大的特点。在称重过程中需要进行计算机同步控制,实现结构物的平稳顶升和下降。大型结构物自动称重以工控机为核心,基于现场总线技术,对压力传感器、位移传感器、状态设置开关、油泵换向阀和油管超高压电磁阀等设备进行网络监控,对多个支点同步协调控制和结构物的精确称重,该系统已在多个油气田工程的建造中得到实际应用。     

体积-表面淬火中加热温度的自动控制

根据表面-体积淬火奥氏体规范的要求，分析了感应加热过程中温度控制的特点和方式，并介绍了采用红外线光导纤维测温和微机控温技术成功用于体积-表面淬火中透热温度的自动控制。     

电磁六极透镜的磁场分布

本文叙述了一种可用于电子显微镜不差矫Ｅ系统的电磁六极透镜的磁场分布研究结果。研究工作系统实验测定磁场分布和理论分析相结合的方法，给出了六极透镜磁感应强度各分量的数值和近似公式，特别着重分析了六极边缘区域的磁场分布情况。     

Sensorless control of synchronous reluctance motors using on‐line parameter identification method not affected by position estimation accuracy

This paper presents a novel on‐line parameter identification method for sensorless control of Synchronous Reluctance Motors (SynRMs). Although conventional sensorless control methods based on mathematical models usually need some complex measurements of motor parameters in advance, the proposed identification method does not require them and can be realized on‐line. The proposed method identifies motor parameters under sensorless control, so rotor position and velocity cannot be used to identify these parameters. However, the proposed method does not need rotor position and velocity, and identified parameters are not affected by these estimation errors. The sensorless control using identified motor parameters is realized, and the effectiveness of the proposed method is verified by experimental results. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 155(3): 62–69, 2006; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20258     

冶金坑内矿多级机站通风系统设计

本文简要论述了冶金坑内矿多级机站通风系统设计的基本原则及计算方法，并给出一个设计实例。     

高频等参数感应(ВИКИЗ)测井技术应用

介绍了高频等参数感应的基本原理。以胜利油田比较典型的 3口井为例 ,详细分析了高频感应资料在划分薄夹层、确定储层流体饱和类型、评价储层电阻率空间分布等方面的应用。研究结果表明 ,高频感应具有极高的纵向分辨率和较好的径向探测特性 ,能够准确计算地层真电阻率、侵入带电阻率及侵入半径 ,通过与常规感应及薄层测井资料的对比 ,论证了高频等参数感应在测井技术应用上的优越性