In Vitro Characterization of Neutralizing Hen Antibodies to Coxsackievirus A16

Coxsackievirus A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). Children aged <5 years are the most affected by CA16 HFMD globally. Although clinical symptoms of CA16 infections are usually mild, severe complications, such as aseptic meningitis or even death, have been recorded. Currently, no vaccine or antiviral therapy for CA16 infection exists. Single-chain variable fragment (scFv) antibodies significantly inhibit viral infection and could be a potential treatment for controlling the infection. In this study, scFv phage display libraries were constructed from splenocytes of a laying hen immunized with CA16-infected lysate. The pComb3X vector containing the scFv genes was introduced into ER2738 Escherichia coli and rescued by helper phages to express scFv molecules. After screening with five cycles of bio-panning, an effective scFv antibody showing favorable binding activity to proteins in CA16-infected lysate on ELISA plates was selected. Importantly, the selected scFv clone showed a neutralizing capability against the CA16 virus and cross-reacted with viral proteins in EV71-infected lysate. Intriguingly, polyclonal IgY antibody not only showed binding specificity against proteins in CA16-infected lysate but also showed significant neutralization activities. Nevertheless, IgY-binding protein did not cross-react with proteins in EV71-infected lysate. These results suggest that the IgY- and scFv-binding protein antibodies provide protection against CA16 viral infection in in vitro assays and may be potential candidates for treating CA16 infection in vulnerable young children.     

可变频率变压器的最大功率传输控制策略

可变频率变压器（VFT）是一种能连接异步电网的新型灵活交流输电系统设备,其可传输功率的容量是重要的性能指标。针对传统控制策略未考虑转差率增大可能导致转子绕组超过额定容量的问题,对不同的转差率和定子无功功率情况下定子有功功率最大值进行研究;详细研究了采用串联变换器的VFT系统运行特性;对不同转差率进行了详细的工况分析。推导出最优定子无功功率给定值与转差率、额定视在功率和实际吸收无功功率之间的数学关系,并提出了VFT的最大功率传输（MPT）控制策略;通过硬件在环实验平台验证提出的MPT控制策略的有效性。实验结果表明,所提MPT控制策略能有效提高VFT的可传输功率,实现VFT的最大功率传输。     

关注区可变的实时仿真方法与平台

交直流混合电网的实时仿真既要考虑电力电子设备的开关特性,又要保证适应较大的电网规模,在实际工程中面临诸多困难.给子网络赋予动态可变的关注标志,对关注子网络采用存储压力小的节点消去法,对非关注子网络采用计算量少的线性组合法,从而保证骨架型节点电压法的实用性.在Xilinx公司的Virtex-7 FPGA VC709开发板中,采用无缝的并行化仿真计算程序对接方式和灵活的结构参数查询方法解决了现场可编程逻辑门阵列资源的紧缺问题,设计了一种关注区可变的实时数字仿真平台.以典型的交直流混合电网为例,仿真验证了所提方法的可行性与所研发平台的有效性.     

减速器的优化设计

减速器常规设计常忽视设计的优化问题，使得机构自重大，成本高。本文根据优化变量的特点，采用了离散变量的组合型优化方法对减速器的设计进行了优化，减少了加工余量，降低了生产成本。     

变频调速传动系统的电磁兼容分析

在变频调速传动系统中,变频器会产生和受到各种电磁干扰。本文分析了变频调速传动系统的主要电磁干扰源和耦合途径,并阐述了抑制其电磁干扰的措施。     

混合驱动五杆机构的滑模变结构控制

建立了混合驱动五杆机构系统的机电耦合动力学模型.采用滑模变结构控制方法实现了对混合输入型五杆机构运动轨迹的精确控制,并给出了仿真结果.该控制方法具有控制算法简单、易于工程实现的优点.     

连续变断面体挤压过程金属的变形特征

采用铅试料，用网格法实验观测连续变断面循环挤压法挤压时金属的流动特征。研究表明，正挤压时，金属在模腔中延伸变形成圆台体，受模具形状影响，变形程度从顶端向底端逐渐减小；圆台体镦粗时金属在高度方向从顶端至底端各层沿径向流动逐渐减小，依次连续变形成圆柱体。这种变形特点使试料在变形过程中逐渐渗透达到累积的效果，并且未出现圆柱体镦粗时的鼓形现象，也未出现因失稳导致的界面叠层。     

PVC塑窗型材锯铣中心设计研究

介绍了已开发成功的PVC塑窗型材锯铣加工中心,从模块化设计、刀具布局设计、绿色设计和适应性设计几个方面对这一新技术产品的设计思想、方法和关键技术进行了探讨.