Cyclic variations in the permeability of the cell wall of Saccharomyces cerevisiae.

To study cell-cycle-related variations in wall permeability of Saccharomyces cerevisiae, two approaches were used. First, an asynchronous culture was fractionated by centrifugal elutriation into subpopulations containing cells of increasing size. The subpopulations represented different stages of the cell cycle as judged by light microscopy. Cell wall porosity increased when these subpopulations became enriched with budded cells. Secondly, synchronous cultures were obtained by releasing MATa cells from alpha-factor induced G1-arrest. These cultures grew synchronously for at least two generations. The cell wall porosity increased sharply in these cultures, shortly before buds became visible and was maximal during the initial stages of bud growth. It decreased in cells which had completed nuclear migration and before abscission of the bud had occurred. The porosity reached its lowest value during abscission and in unbudded cells. We examined the incorporation of mannoproteins into the wall during the cell cycle. SDS-extractable mannoproteins were incorporated continuously. However, the incorporation of glucanase-extractable mannoproteins, which are known to affect cell wall porosity, showed cyclic oscillations and reached its maximum after nuclear migration. This coincided with a rapid decrease in cell wall porosity, indicating that glucanase-extractable mannoproteins might contribute to this decrease.     

浅谈赣抚大堤樟防1标射水造墙的接缝技术和处理措施

介绍射水造墙的接缝原理，并把自己在施工过程中为确保接缝质量达到设计要求而总结出一些经验和处理措施提出为一起探讨。     

砖混结构现浇楼板的裂缝分析及预防措施

针对目前多层住宅采用砖混结构现浇钢筋混凝土产生裂缝的情况，分析了其产生原因，提出了预防措施。指出这些预防措施可使裂缝得到有效控制。     

高层建筑外墙干挂花岗岩质量监控

结合安业大厦施工实例，介绍了高层建筑大面积干挂花岗岩的施工过程。为了确保工程一次成活达优，对实施工程质量进行全方位监控。首先确定质量监控的目的，明确专项管理组织及专项负责人，严格监理指令制度。同时针对监控的重点、难点和关键点，组织各方实现工程质量的监控。     

泄压注浆法在治理小型突水中的运用

七煤集团建设工程公司在施工辽阳铧子石膏矿主、风井井筒时 ,多次采用泄压注浆法成功通过中小型含水层 ,对小型突水进行了有效封堵 ,取得了较好的经济效益     

我国水利水电混凝土防渗墙的发展

４０年来，我国水利水电混凝土防渗技术，从无到有逐渐发展至今，已能够在各种特殊地层中修建各种用途的混凝土防渗墙。通过大量实践，积累了丰富经验。在科学研究、勘测设计和施工技术及现代化管理方面也获得很大进展，解决了在复杂地质条件下建坝所遇到的规模较大的常规地基处理技术，也掌握了特殊地基处理的某些关键技术。     

埋地管道不开挖地面腐蚀检测技术在胜利油田纯梁采油厂的应用

综合利用管道探测、防腐保温层破损点定位、防腐保温层性能检测评价、管道阳极倾向点识别和管壁剩余厚度检测评价等技术对胜利油田纯梁采油厂的纯梁首站—史南站19.92km输油管道进行了不开挖腐蚀检测。通过检测评价,对被检管道的走向与埋深进行了精确定位,并确定了需更换、维修及重点监控与维护的具体部位,为被检管道的更换、维修及维护提供了科学依据,对保障管道安全运行具有重要意义。     

自锁式景观砌块加筋挡土结构的设计与应用

介绍砌块仿花岗岩挡土结构的特点和砌块形状,针对砌块挡土结构在我国刚开始应用,没有设计、施工规范的现状,依托可以借鉴的加筋挡土结构和浆砌块石挡土结构设计规范,提出了自锁式砌块加筋挡土结构设计中的地面荷载强度及土压力计算方法、砌块层间稳定验算方法、加筋长度和间距验算方法、挡土结构整体稳定验算方法和施工过程中需要注意的主要问题,为自锁式砌块加筋挡土结构设计人员提供了设计验算内容、验算方法和安全系数取值范围,为现场施工和监理人员提供参考,并为该类型的挡土结构推广应用提供技术支持。     

The development of MHD cold wall channels in the oil-fired condition

The durability of a cold wall MHD generator consisting of metal electrodes and alumina-coated peg insulators was experimentally examined in the oil-fired MHD condition with the additive of SO₂ corresponding to S = 0.54 wt%. The structure of the electrode wall and various anode and cathode materials were tested, with particular reference to developing long-life electrodes. It was clarified that Pt, SHOMAC (28.8 Cr−1.9 Mo) and SUS-304 as anodes and W---Cu (70 W−30 Cu) and WC---Ag (60 WC−40 Ag) alloys as cathodes were promising for durability and that the structure of an anode divided into two elements connected to each other with an SnO₂ resistor was very useful for uniformity of the anode corrosion pattern and the inter-anode voltages. It is reasonable to expect, from the test results, that the lifetime of a cold wall MHD generator in an oil-fired commercial plant will be over 4000 h, and therefore, a foundation for its commercial use has been established.     

A protein which masks galactose receptor mediated phage susceptibility in Lactococcus lactis subsp. lactis MPL56

A 28.5-kb plasmid, isolated from Lactococcus lactis subsp. lactis MPL56, causes complete inhibition of four lactococcal phages. Cell wall characteristics of wild-type strain MPL56 were compared with its 28.5 kb plasmid-cured, phage-sensitive derivative MPL56-22. After proteolytic enzyme treatments, adsorption of phages occurred at high levels, an example is 94.6–98.5% in MPL56 cells. Analysis of cell wall extracts of MPL56-22 by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) indicated that the only difference between strains was the 55.4 kDa band in protein patterns of MPL56. Adsorption of the four phages was completely inhibited when MPL56-22 cells were subjected to SDS, Triton-X-100, HCl and NaOH treatments. Lectins that were specific for glucose/mannose and N-acetylglucosamine did not prevent adsorption of phages in cell wall extracts of MPL 56-22. However a lectin specific for galactose (MCA; Momordica charantia ) completely inhibited adsorption of these phages in cell wall extracts of MPL56-22. HPLC patterns of cell wall carbohydrates of MPL56-22 and its HCl treated preparations showed that the most prevalent difference was the galactose on untreated MPL56-22 cell wall chromatograms.